{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T07:57:42Z","timestamp":1793692662721,"version":"4.5.1"},"reference-count":52,"publisher":"American Society for Cell Biology (ASCB)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2016,1,15]]},"abstract":"<jats:p>Arl13b belongs to the ADP-ribosylation factor family within the Ras superfamily of regulatory GTPases. Mutations in Arl13b cause Joubert syndrome, which is characterized by congenital cerebellar ataxia, hypotonia, oculomotor apraxia, and mental retardation. Arl13b is highly enriched in cilia and is required for ciliogenesis in multiple organs. Nevertheless, the precise role of Arl13b remains elusive. Here we report that the exocyst subunits Sec8, Exo70, and Sec5 bind preferentially to the GTP-bound form of Arl13b, consistent with the exocyst being an effector of Arl13b. Moreover, we show that Arl13b binds directly to Sec8 and Sec5. In zebrafish, depletion of arl13b or the exocyst subunit sec10 causes phenotypes characteristic of defective cilia, such as curly tail up, edema, and abnormal pronephric kidney development. We explored this further and found a synergistic genetic interaction between arl13b and sec10 morphants in cilia-dependent phenotypes. Through conditional deletion of Arl13b or Sec10 in mice, we found kidney cysts and decreased ciliogenesis in cells surrounding the cysts. Moreover, we observed a decrease in Arl13b expression in the kidneys from Sec10 conditional knockout mice. Taken together, our results indicate that Arl13b and the exocyst function together in the same pathway leading to functional cilia.<\/jats:p>","DOI":"10.1091\/mbc.e15-02-0061","type":"journal-article","created":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T22:44:02Z","timestamp":1447886642000},"page":"308-320","source":"Crossref","is-referenced-by-count":82,"title":["Arl13b and the exocyst interact synergistically in ciliogenesis"],"prefix":"10.1091","volume":"27","author":[{"given":"Cec\u00edlia","family":"Seixas","sequence":"first","affiliation":[{"name":"CEDOC, NOVA Medical School\/Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Nova de Lisboa, 1169-056 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Young","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical University of South Carolina, Charleston, SC 29425"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noemi","family":"Polgar","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Biochemistry, and Physiology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole L.","family":"Umberger","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30022"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"East","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30022"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Zuo","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical University of South Carolina, Charleston, SC 29425"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Moreiras","sequence":"additional","affiliation":[{"name":"CEDOC, NOVA Medical School\/Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Nova de Lisboa, 1169-056 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania","family":"Ghossoub","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Canc\u00e9rologie de Marseille, INSERM, UMR7258, 13009 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Benmerah","sequence":"additional","affiliation":[{"name":"INSERM UMR 1163, Laboratory of Inherited Kidney Diseases, 75015 Paris, France"},{"name":"Paris Descartes-Sorbonne Paris Cit\u00e9 University, Imagine Institute, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Kahn","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30022"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Fogelgren","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Biochemistry, and Physiology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96813"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Caspary","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30022"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua H.","family":"Lipschutz","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical University of South Carolina, Charleston, SC 29425"},{"name":"Department of Medicine, RHJ Veterans Affairs Medical Center, Charleston, SC 29425"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duarte C.","family":"Barral","sequence":"additional","affiliation":[{"name":"CEDOC, NOVA Medical School\/Faculdade de Ci\u00eancias M\u00e9dicas, Universidade Nova de Lisboa, 1169-056 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"F495","DOI":"10.1152\/ajprenal.00198.2010","volume":"299","author":"Babbey CM","year":"2010","journal-title":"Am J Physiol Renal Physiol"},{"key":"B2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1146\/annurev.genom.7.080505.115610","volume":"7","author":"Badano JL","year":"2006","journal-title":"Annu Rev Genomics Hum Genet"},{"key":"B3","doi-asserted-by":"crossref","first-page":"21354","DOI":"10.1073\/pnas.1218272110","volume":"109","author":"Barral DC","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"key":"B4","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1007\/s00018-006-6180-x","volume":"63","author":"Blacque OE","year":"2006","journal-title":"Cell Mol Life Sci C"},{"key":"B5","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.ajhg.2008.06.023","author":"Cantagrel V","year":"2008","journal-title":"Am J Hum Genet"},{"key":"B6","doi-asserted-by":"crossref","first-page":"2709","DOI":"10.1242\/jcs.143446","volume":"127","author":"Casalou C","year":"2014","journal-title":"J Cell Sci"},{"key":"B7","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.devcel.2007.03.004","volume":"12","author":"Caspary T","year":"2007","journal-title":"Dev Cell"},{"key":"B8","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1083\/jcb.200908133","volume":"188","author":"Cevik S","year":"2010","journal-title":"J Cell Biol"},{"key":"B9","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.tcb.2011.03.006","volume":"21","author":"Das A","year":"2011","journal-title":"Trends Cell Biol"},{"key":"B10","first-page":"138ra78","volume":"4","author":"Dixon-Salazar TJ","year":"2012","journal-title":"Sci Transl Med"},{"key":"B11","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/nrm3117","volume":"12","author":"Donaldson JG","year":"2011","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B12","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/dvg.20226","volume":"44","author":"Dubois NC","year":"2006","journal-title":"Genesis"},{"key":"B13","doi-asserted-by":"crossref","first-page":"4033","DOI":"10.1242\/dev.036350","volume":"136","author":"Duldulao Na","year":"2009","journal-title":"Development"},{"key":"B14","doi-asserted-by":"crossref","first-page":"15602","DOI":"10.1074\/jbc.M111.333245","volume":"287","author":"Feng S","year":"2012","journal-title":"J Biol Chem"},{"key":"B15","doi-asserted-by":"crossref","first-page":"e1001361","DOI":"10.1371\/journal.pgen.1001361","volume":"7","author":"Fogelgren B","year":"2011","journal-title":"PLoS Genet"},{"key":"B16","doi-asserted-by":"crossref","first-page":"e0129346","DOI":"10.1371\/journal.pone.0129346","volume":"10","author":"Fogelgren B","year":"2015","journal-title":"PLoS One"},{"key":"B17","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1006\/dbio.1997.8727","volume":"192","author":"Friedrich Ga","year":"1997","journal-title":"Dev Biol"},{"key":"B18","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1007\/s00467-010-1697-5","volume":"26","author":"Gascue C","year":"2011","journal-title":"Pediatr Nephrol"},{"key":"B19","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1093\/emboj\/18.4.1071","volume":"18","author":"Guo W","year":"1999","journal-title":"EMBO J"},{"key":"B20","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1038\/nrc3458","volume":"13","author":"Harvey KF","year":"2013","journal-title":"Nat Rev Cancer"},{"key":"B21","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.ceb.2009.04.007","volume":"21","author":"He B","year":"2009","journal-title":"Curr Opin Cell Biol"},{"key":"B22","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/j.devcel.2012.09.019","volume":"23","author":"Higginbotham H","year":"2012","journal-title":"Dev Cell"},{"key":"B23","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1681\/ASN.2008050456","volume":"20","author":"Hildebrandt F","year":"2009","journal-title":"J Am Soc Nephrol"},{"key":"B24","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1126\/science.271.5250.810","volume":"271","author":"Joneson T","year":"1996","journal-title":"Science"},{"key":"B25","doi-asserted-by":"crossref","first-page":"6346","DOI":"10.1073\/pnas.1002401107","volume":"107","author":"Kn\u00f6dler A","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"B26","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1242\/dev.01772","volume":"132","author":"Kramer-Zucker AG","year":"2005","journal-title":"Development"},{"key":"B27","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/S0014-5793(00)02325-5","volume":"487","author":"Kuai J","year":"2000","journal-title":"FEBS Lett"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e10-12-0994"},{"key":"B29","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1038\/cr.2008.37","volume":"18","author":"Lawrence MC","year":"2008","journal-title":"Cell Res"},{"key":"B30","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1038\/ng.1078","volume":"44","author":"Lee JE","year":"2012","journal-title":"Nat Genet"},{"key":"B31","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1002\/jcb.24116","volume":"113","author":"Li Y","year":"2012","journal-title":"J Cell Biochem"},{"key":"B32","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/emboj.2008.267","volume":"28","author":"Mazelova J","year":"2009","journal-title":"EMBO J"},{"key":"B33","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1242\/jcs.039982","volume":"122","author":"Mazelova J","year":"2009","journal-title":"J Cell Sci"},{"key":"B34","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1146\/annurev.cellbio.042308.113337","volume":"26","author":"Nachury MV","year":"2010","journal-title":"Annu Rev Cell Dev Biol"},{"key":"B35","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1681\/ASN.2004090800","volume":"17","author":"Omori S","year":"2006","journal-title":"J Am Soc Nephrol"},{"key":"B36","doi-asserted-by":"crossref","first-page":"C190","DOI":"10.1152\/ajpcell.00011.2015","volume":"309","author":"Polgar N","year":"2015","journal-title":"Am J Physiol Cell Physiol"},{"key":"B37","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1083\/jcb.200305029","volume":"163","author":"Prigent M","year":"2003","journal-title":"J Cell Biol"},{"key":"B38","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.bbrc.2004.04.165","volume":"319","author":"Rogers KK","year":"2004","journal-title":"Biochem Biophys Res Commun"},{"key":"B39","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1016\/S1474-4422(13)70136-4","volume":"12","author":"Romani M","year":"2013","journal-title":"Lancet Neurol"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.9.7.1725"},{"key":"B41","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1097\/01.ASN.0000016444.90348.50","volume":"13","author":"Shao X","year":"2002","journal-title":"J Am Soc Nephrol"},{"key":"B42","doi-asserted-by":"crossref","first-page":"4062","DOI":"10.1242\/dev.082321","volume":"139","author":"Su C-Y","year":"2012","journal-title":"Development"},{"key":"B43","doi-asserted-by":"crossref","first-page":"4085","DOI":"10.1242\/dev.01240","volume":"131","author":"Sun Z","year":"2004","journal-title":"Development"},{"key":"B44","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/ejhg.2014.156","volume":"23","author":"Thomas S","year":"2014","journal-title":"Eur J Hum Genet"},{"key":"B45","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/12703","volume":"23","author":"Tronche F","year":"1999","journal-title":"Nat Genet"},{"key":"B46","doi-asserted-by":"crossref","first-page":"4057","DOI":"10.1038\/emboj.2012.253","volume":"31","author":"Wang J","year":"2012","journal-title":"EMBO J"},{"key":"B47","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1073\/pnas.1018823108","volume":"108","author":"Westlake CJ","year":"2011","journal-title":"Proc Natl Acad Sci USA"},{"key":"B48","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1083\/jcb.200703047","volume":"178","author":"Yoshimura S-I","year":"2007","journal-title":"J Cell Biol"},{"key":"B49","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.cell.2012.01.015","volume":"148","author":"Yu X","year":"2012","journal-title":"Cell"},{"key":"B50","doi-asserted-by":"crossref","first-page":"43027","DOI":"10.1074\/jbc.M402264200","volume":"279","author":"Zhang X-M","year":"2004","journal-title":"J Biol Chem"},{"key":"B51","doi-asserted-by":"crossref","first-page":"22469","DOI":"10.1074\/jbc.M111.238469","volume":"286","author":"Zuo X","year":"2011","journal-title":"J Biol Chem"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e08-07-0772"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2019,7,14]],"date-time":"2019-07-14T08:31:58Z","timestamp":1563093118000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.e15-02-0061"}},"subtitle":[],"editor":[{"given":"Wallace","family":"Marshall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,1,15]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,1,15]]}},"alternative-id":["10.1091\/mbc.e15-02-0061"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e15-02-0061","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,15]]}}}