{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,4]],"date-time":"2024-08-04T19:14:09Z","timestamp":1722798849726},"reference-count":44,"publisher":"American Society for Cell Biology (ASCB)","issue":"23","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2016,11,15]]},"abstract":"<jats:p>Epidermal growth factor receptor (EGFR) signaling is essential for animal development, and increased signaling underlies many human cancers. Identifying the genes and cellular processes that regulate EGFR signaling in vivo will help to elucidate how this pathway can become inappropriately activated. Caenorhabditis elegans vulva development provides an in vivo model to genetically dissect EGFR signaling. Here we identified a mutation in dhc-1, the heavy chain of the cytoplasmic dynein minus end\u2013directed microtubule motor, in a genetic screen for regulators of EGFR signaling. Despite the many cellular functions of dynein, DHC-1 is a strong negative regulator of EGFR signaling during vulva induction. DHC-1 is required in the signal-receiving cell and genetically functions upstream or in parallel to LET-23 EGFR. LET-23 EGFR accumulates in cytoplasmic foci in dhc-1 mutants, consistent with mammalian cell studies in which dynein is shown to regulate late endosome trafficking of EGFR with the Rab7 GTPase. However, we found different distributions of LET-23 EGFR foci in rab-7 versus dhc-1 mutants, suggesting that dynein functions at an earlier step of LET-23 EGFR trafficking to the lysosome than RAB-7. Our results demonstrate an in vivo role for dynein in limiting LET-23 EGFR signaling via endosomal trafficking.<\/jats:p>","DOI":"10.1091\/mbc.e15-11-0757","type":"journal-article","created":{"date-parts":[[2016,9,22]],"date-time":"2016-09-22T02:49:21Z","timestamp":1474512561000},"page":"3771-3779","source":"Crossref","is-referenced-by-count":4,"title":["Dynein-mediated trafficking negatively regulates LET-23 EGFR signaling"],"prefix":"10.1091","volume":"27","author":[{"given":"Olga","family":"Skorobogata","sequence":"first","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine, and Department of Anatomy and Cell Biology, McGill University, and Program in Experimental Therapeutics and Metabolism, Centre for Translational Biology, Research Institute of the McGill University Health Centre, Montreal, QC H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jassy","family":"Meng","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine, and Department of Anatomy and Cell Biology, McGill University, and Program in Experimental Therapeutics and Metabolism, Centre for Translational Biology, Research Institute of the McGill University Health Centre, Montreal, QC H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberley","family":"Gauthier","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine, and Department of Anatomy and Cell Biology, McGill University, and Program in Experimental Therapeutics and Metabolism, Centre for Translational Biology, Research Institute of the McGill University Health Centre, Montreal, QC H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian E.","family":"Rocheleau","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine, and Department of Anatomy and Cell Biology, McGill University, and Program in Experimental Therapeutics and Metabolism, Centre for Translational Biology, Research Institute of the McGill University Health Centre, Montreal, QC H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1042\/BST0391169","volume":"39","author":"Allan VJ","year":"2011","journal-title":"Biochem Soc Trans"},{"key":"B2","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1242\/dev.126.9.1947","volume":"126","author":"Ambros V","year":"1999","journal-title":"Development"},{"key":"B3","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1002\/j.1460-2075.1994.tb06269.x","volume":"13","author":"Aroian RV","year":"1994","journal-title":"EMBO J"},{"key":"B4","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1093\/genetics\/128.2.251","volume":"128","author":"Aroian RV","year":"1991","journal-title":"Genetics"},{"key":"B5","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.devcel.2012.12.001","volume":"24","author":"Barkoulas M","year":"2013","journal-title":"Dev Cell"},{"key":"B6","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1074\/jbc.M112.417766","volume":"288","author":"BasuRay S","year":"2013","journal-title":"J Biol Chem"},{"key":"B7","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1126\/science.1055642","volume":"291","author":"Berset T","year":"2001","journal-title":"Science"},{"key":"B8","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","volume":"77","author":"Brenner S","year":"1974","journal-title":"Genetics"},{"key":"B9","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/1471-2164-6-118","volume":"6","author":"Davis MW","year":"2005","journal-title":"BMC Genomics"},{"key":"B10","doi-asserted-by":"crossref","first-page":"2385","DOI":"10.1101\/gad.1687508","volume":"22","author":"Gassmann R","year":"2008","journal-title":"Genes Dev"},{"key":"B11","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1083\/jcb.147.1.135","volume":"147","author":"Gonczy P","year":"1999","journal-title":"J Cell Biol"},{"key":"B12","doi-asserted-by":"crossref","first-page":"e1004341","DOI":"10.1371\/journal.pgen.1004341","volume":"10","author":"Haag A","year":"2014","journal-title":"PLoS Genet"},{"key":"B13","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1101\/gad.11.20.2715","volume":"11","author":"Hajnal A","year":"1997","journal-title":"Genes Dev"},{"key":"B14","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/S1534-5807(02)00327-1","volume":"3","author":"Hamill DR","year":"2002","journal-title":"Dev Cell"},{"key":"B15","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1042\/BST0391202","volume":"39","author":"Horgan CP","year":"2011","journal-title":"Biochem Soc Trans"},{"key":"B16","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1042\/BST0391179","volume":"39","author":"Hunt SD","year":"2011","journal-title":"Biochem Soc Trans"},{"key":"B17","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1242\/dev.00924","volume":"131","author":"Hwang BJ","year":"2004","journal-title":"Development"},{"key":"B18","doi-asserted-by":"crossref","first-page":"2643","DOI":"10.1242\/jcs.027144","volume":"121","author":"Iyadurai SJ","year":"2008","journal-title":"J Cell Sci"},{"key":"B19","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1083\/jcb.200606077","volume":"176","author":"Johansson M","year":"2007","journal-title":"J Cell Biol"},{"key":"B20","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1093\/genetics\/139.4.1553","volume":"139","author":"Jongeward GD","year":"1995","journal-title":"Genetics"},{"key":"B21","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/S0092-8674(00)81735-3","volume":"94","author":"Kaech SM","year":"1998","journal-title":"Cell"},{"key":"B22","doi-asserted-by":"crossref","first-page":"RESEARCH0002","DOI":"10.1186\/gb-2001-2-2-reports0002","volume":"2","author":"Kamath RS","year":"2001","journal-title":"Genome Biol"},{"key":"B23","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0092-8674(88)80024-2","volume":"52","author":"Kemphues KJ","year":"1988","journal-title":"Cell"},{"key":"B24","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/0012-1606(81)90152-4","volume":"87","author":"Kimble J","year":"1981","journal-title":"Dev Biol"},{"key":"B25","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1002\/cm.970320104","volume":"32","author":"Lye RJ","year":"1995","journal-title":"Cell Motility Cytoskeleton"},{"key":"B26","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.gene.2005.10.018","volume":"366","author":"Normanno N","year":"2006","journal-title":"Gene"},{"key":"B27","doi-asserted-by":"crossref","first-page":"e128","DOI":"10.1371\/journal.pgen.0030128","volume":"3","author":"O\u2019Rourke SM","year":"2007","journal-title":"PLoS Genet"},{"key":"B28","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.gene.2007.06.020","volume":"400","author":"Qadota H","year":"2007","journal-title":"Gene"},{"key":"B29","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1038\/nrm3667","volume":"14","author":"Roberts AJ","year":"2013","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.gde.2015.01.006","volume":"32","author":"Schmid T","year":"2015","journal-title":"Curr Opin Genet Dev"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-06-0523"},{"key":"B32","doi-asserted-by":"crossref","first-page":"e1004728","DOI":"10.1371\/journal.pgen.1004728","volume":"10","author":"Skorobogata O","year":"2014","journal-title":"PLoS Genet"},{"key":"B33","doi-asserted-by":"crossref","first-page":"e36489","DOI":"10.1371\/journal.pone.0036489","volume":"7","author":"Skorobogata O","year":"2012","journal-title":"PLoS One"},{"key":"B34","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.yexcr.2008.07.029","volume":"315","author":"Sorkin A","year":"2009","journal-title":"Exp Cell Res"},{"key":"B35","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1038\/nrm2728","volume":"10","author":"Stenmark H","year":"2009","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B36","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/0012-1606(77)90158-0","volume":"56","author":"Sulston JE","year":"1977","journal-title":"Dev Biol"},{"key":"B37","first-page":"1","author":"Sundaram MV","year":"2013","journal-title":"WormBook 2013(Jul 11)"},{"key":"B38","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0092-8674(00)81644-X","volume":"99","author":"Tabara H","year":"1999","journal-title":"Cell"},{"key":"B39","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/S0092-8674(00)81186-1","volume":"93","author":"Tan PB","year":"1998","journal-title":"Cell"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e07-02-0098"},{"key":"B41","doi-asserted-by":"crossref","first-page":"12110","DOI":"10.1074\/jbc.M809277200","volume":"284","author":"Vanlandingham PA","year":"2009","journal-title":"J Biol Chem"},{"key":"B42","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1006\/dbio.1999.9357","volume":"212","author":"Wang M","year":"1999","journal-title":"Dev Biol"},{"key":"B43","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1126\/science.7652556","volume":"269","author":"Yoon CH","year":"1995","journal-title":"Science"},{"key":"B44","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/genetics\/131.2.321","volume":"131","author":"Zetka MC","year":"1992","journal-title":"Genetics"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1091\/mbc.E15-11-0757","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T23:45:47Z","timestamp":1657323947000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.e15-11-0757"}},"subtitle":[],"editor":[{"given":"Jeffrey D.","family":"Hardin","sequence":"additional","affiliation":[{"name":"University of Wisconsin"}],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,11,15]]},"references-count":44,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2016,11,15]]}},"alternative-id":["10.1091\/mbc.e15-11-0757"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e15-11-0757","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,15]]}}}