{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:21:05Z","timestamp":1791429665295,"version":"4.3.3"},"reference-count":49,"publisher":"American Society for Cell Biology (ASCB)","issue":"16","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2016,8,15]]},"abstract":"<jats:p>Leiomodin is a potent actin nucleator related to tropomodulin, a capping protein localized at the pointed end of the thin filaments. Mutations in leiomodin-3 are associated with lethal nemaline myopathy in humans, and leiomodin-2\u2013knockout mice present with dilated cardiomyopathy. The arrangement of the N-terminal actin- and tropomyosin-binding sites in leiomodin is contradictory and functionally not well understood. Using one-dimensional nuclear magnetic resonance and the pointed-end actin polymerization assay, we find that leiomodin-2, a major cardiac isoform, has an N-terminal actin-binding site located within residues 43\u201390. Moreover, for the first time, we obtain evidence that there are additional interactions with actin within residues 124\u2013201. Here we establish that leiomodin interacts with only one tropomyosin molecule, and this is the only site of interaction between leiomodin and tropomyosin. Introduction of mutations in both actin- and tropomyosin-binding sites of leiomodin affected its localization at the pointed ends of the thin filaments in cardiomyocytes. On the basis of our new findings, we propose a model in which leiomodin regulates actin poly\u00admerization dynamics in myocytes by acting as a leaky cap at thin filament pointed ends.<\/jats:p>","DOI":"10.1091\/mbc.e16-03-0200","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T04:13:33Z","timestamp":1466050413000},"page":"2565-2575","source":"Crossref","is-referenced-by-count":28,"title":["The N-terminal tropomyosin- and actin-binding sites are important for leiomodin 2\u2019s function"],"prefix":"10.1091","volume":"27","author":[{"given":"Thu","family":"Ly","sequence":"first","affiliation":[{"name":"Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-6515"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Moroz","sequence":"additional","affiliation":[{"name":"Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 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