{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T06:30:05Z","timestamp":1774593005969,"version":"3.50.1"},"reference-count":64,"publisher":"American Society for Cell Biology (ASCB)","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>Retrolinkin, a neuronal membrane protein, coordinates with endophilin A1 and mediates early endocytic trafficking and signal transduction of the ligand\u2013receptor complex formed between brain-derived neurotrophic factor (BDNF) and its receptor, tropomyosin-related kinase B (TrkB), in dendrites of CNS neurons. Here we report that retrolinkin interacts with the CYFIP1\/2 subunit of the WAVE1 complex, a member of the WASP\/WAVE family of nucleation-promoting factors that binds and activates the Arp2\/3 complex to promote branched actin polymerization. WAVE1, not N-WASP, is required for BDNF-induced TrkB endocytosis and dendrite outgrowth. Disruption of the interaction between retrolinkin and CYFIP1\/2 impairs recruitment of WAVE1 to neuronal plasma membrane upon BDNF addition and blocks internalization of activated TrkB. We also show that WAVE1-mediated endocytosis of BDNF-activated TrkB is actin dependent and clathrin independent. These results not only reveal the mechanistic role of retrolinkin in BDNF\u2013TrkB endocytosis, but also indicate that WASP\/WAVE-dependent actin polymerization during endocytosis is regulated by cell type\u2013specific and cargo-specific modulators.<\/jats:p>","DOI":"10.1091\/mbc.e16-05-0326","type":"journal-article","created":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T02:39:09Z","timestamp":1473302349000},"page":"3342-3356","source":"Crossref","is-referenced-by-count":29,"title":["Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF-induced TrkB endocytosis and dendrite outgrowth"],"prefix":"10.1091","volume":"27","author":[{"given":"Chenchang","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China"},{"name":"CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of 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