{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T05:52:08Z","timestamp":1778737928391,"version":"3.51.4"},"reference-count":31,"publisher":"American Society for Cell Biology (ASCB)","issue":"23","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2017,11,7]]},"abstract":"<jats:p>We present a computational model to test a \u201cpolarity sorting\u201d mechanism for microtubule (MT) organization in developing axons. We simulate the motor-based axonal transport of short MTs to test the hypothesis that immobilized cytoplasmic dynein motors transport short MTs with their plus ends leading, so \u201cmal-oriented\u201d MTs with minus-end-out are transported toward the cell body while \u201ccorrectly\u201d oriented MTs are transported in the anterograde direction away from the soma. We find that dynein-based transport of short MTs can explain the predominately plus-end-out polarity pattern of axonal MTs but that transient attachments of plus-end-directed motor proteins and nonmotile cross-linker proteins are needed to explain the frequent pauses and occasional reversals observed in live-cell imaging of MT transport. Static cross-linkers increase the likelihood of a stalled \u201ctug-of-war\u201d between retrograde and anterograde forces on the MT, providing an explanation for the frequent pauses of short MTs and the immobility of longer MTs. We predict that inhibition of the proposed static cross-linker will produce disordered transport of short MTs and increased mobility of longer MTs. We also predict that acute inhibition of cytoplasmic dynein will disrupt the polarity sorting of MTs by increasing the likelihood of \u201cincorrect\u201d sorting of MTs by plus-end-directed motors.<\/jats:p>","DOI":"10.1091\/mbc.e17-06-0380","type":"journal-article","created":{"date-parts":[[2017,10,5]],"date-time":"2017-10-05T00:55:25Z","timestamp":1507164925000},"page":"3271-3285","source":"Crossref","is-referenced-by-count":13,"title":["Polarity sorting of axonal microtubules: a computational study"],"prefix":"10.1091","volume":"28","author":[{"given":"Erin M.","family":"Craig","sequence":"first","affiliation":[{"name":"Department of Physics, Central Washington University, Ellensburg, WA 98926"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard T.","family":"Yeung","sequence":"additional","affiliation":[{"name":"Department of Physics, Central Washington University, Ellensburg, WA 98926"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anand 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