{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:51:49Z","timestamp":1790985109314,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Cell Biology (ASCB)","issue":"16","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2019,7,22]]},"abstract":"<jats:p> Dishevelled-Associating Protein with a high frequency of LEucines (DAPLE) belongs to a group of unconventional activators of heterotrimeric G-proteins that are cytoplasmic factors rather than membrane proteins of the G-protein\u2013coupled receptor superfamily. During neurulation, DAPLE localizes to apical junctions of neuroepithelial cells and promotes apical cell constriction via G-protein activation. While junctional localization of DAPLE is necessary for this function, the factors it associates with at apical junctions or how they contribute to DAPLE-mediated apical constriction are unknown. MPDZ is a multi-PDZ (PSD95\/DLG1\/ZO-1) domain scaffold present at apical cell junctions whose mutation in humans is linked to nonsyndromic congenital hydrocephalus (NSCH). DAPLE contains a PDZ-binding motif (PBM) and is also mutated in human NSCH, so we investigated the functional relationship between both proteins. DAPLE colocalized with MPDZ at apical cell junctions and bound directly to the PDZ3 domain of MPDZ via its PBM. Much like DAPLE, MPDZ is induced during neurulation in Xenopus and is required for apical constriction of neuroepithelial cells and subsequent neural plate bending. MPDZ depletion also blunted DAPLE-\u00admediated apical constriction of cultured cells. These results show that DAPLE and MPDZ, two factors genetically linked to NSCH, function as cooperative partners at apical junctions and are required for proper tissue remodeling during early stages of neurodevelopment. <\/jats:p>","DOI":"10.1091\/mbc.e19-02-0091","type":"journal-article","created":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T17:10:41Z","timestamp":1562173841000},"page":"1900-1910","source":"Crossref","is-referenced-by-count":28,"title":["DAPLE and MPDZ bind to each other and cooperate to promote apical cell constriction"],"prefix":"10.1091","volume":"30","author":[{"given":"Arthur","family":"Marivin","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikel","family":"Garcia-Marcos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"2372","DOI":"10.1128\/MCB.01505-08","volume":"29","author":"Adachi M","year":"2009","journal-title":"Mol Cell Biol"},{"key":"B2","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1136\/jmedgenet-2012-101294","volume":"50","author":"Al-Dosari MS","year":"2013","journal-title":"J Med Genet"},{"key":"B3","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.1016\/j.yexcr.2013.07.011","volume":"319","author":"Assemat E","year":"2013","journal-title":"Exp Cell Res"},{"key":"B4","doi-asserted-by":"crossref","first-page":"e07091","DOI":"10.7554\/eLife.07091","volume":"4","author":"Aznar N","year":"2015","journal-title":"eLife"},{"key":"B5","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1472-6750-6-12","volume":"6","author":"Cabrita LD","year":"2006","journal-title":"BMC Biotechnol"},{"key":"B6","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1038\/nsb0498-317","volume":"5","author":"Daniels DL","year":"1998","journal-title":"Nat Struct Biol"},{"key":"B7","doi-asserted-by":"crossref","first-page":"15163","DOI":"10.1038\/ncomms15163","volume":"8","author":"de Opakua AI","year":"2017","journal-title":"Nat Commun"},{"key":"B8","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1021\/acs.biochem.7b00845","volume":"57","author":"DiGiacomo V","year":"2018","journal-title":"Biochemistry"},{"key":"B9","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1136\/jmedgenet-2012-101190","volume":"49","author":"Drielsma A","year":"2012","journal-title":"J Med Genet"},{"key":"B10","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1159\/000319859","volume":"1","author":"Ekici AB","year":"2010","journal-title":"Mol Syndromol"},{"key":"B11","doi-asserted-by":"crossref","first-page":"890","DOI":"10.15252\/emmm.201606430","volume":"9","author":"Feldner A","year":"2017","journal-title":"EMBO Mol Med"},{"key":"B12","doi-asserted-by":"crossref","first-page":"12765","DOI":"10.1074\/jbc.M109.045161","volume":"285","author":"Garcia-Marcos M","year":"2010","journal-title":"J Biol Chem"},{"key":"B13","doi-asserted-by":"crossref","first-page":"3178","DOI":"10.1073\/pnas.0900294106","volume":"106","author":"Garcia-Marcos M","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"B14","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1146\/annurev.bi.56.070187.003151","volume":"56","author":"Gilman AG","year":"1987","journal-title":"Annu Rev Biochem"},{"key":"B15","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nature21348","volume":"541","author":"Gilmour D","year":"2017","journal-title":"Nature"},{"key":"B16","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1097\/NEN.0000000000000203","volume":"74","author":"Guerra MM","year":"2015","journal-title":"J Neuropathol Exp Neurol"},{"key":"B17","doi-asserted-by":"crossref","first-page":"4249","DOI":"10.1242\/dev.151282","volume":"144","author":"Heer NC","year":"2017","journal-title":"Development"},{"key":"B18","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1038\/35019573","volume":"2","author":"Joberty G","year":"2000","journal-title":"Nat Cell Biol"},{"key":"B19","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.1016\/j.mod.2005.05.003","volume":"122","author":"Kobayashi H","year":"2005","journal-title":"Mech Dev"},{"key":"B20","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1111\/gbb.12171","volume":"13","author":"Kruse LC","year":"2014","journal-title":"Genes Brain Behav"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e07-01-0073"},{"key":"B22","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1083\/jcb.201811174","volume":"218","author":"Marivin A","year":"2019","journal-title":"J Cell Biol"},{"key":"B23","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1242\/dev.102228","volume":"141","author":"Martin AC","year":"2014","journal-title":"Development"},{"key":"B24","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/0092-8674(82)90272-0","volume":"30","author":"Newport J","year":"1982","journal-title":"Cell"},{"key":"B25","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1242\/dev.145904","volume":"144","author":"Nikolopoulou E","year":"2017","journal-title":"Development"},{"key":"B26","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1242\/bio.201511676","volume":"4","author":"Ossipova O","year":"2015","journal-title":"Biol Open"},{"key":"B27","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.devcel.2015.10.010","volume":"35","author":"Peshkin L","year":"2015","journal-title":"Dev Cell"},{"key":"B28","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1159\/000453074","volume":"52","author":"Rodriguez EM","year":"2017","journal-title":"Pediatr Neurosurg"},{"key":"B29","doi-asserted-by":"crossref","first-page":"231","DOI":"10.4067\/S0716-97602012000300005","volume":"45","author":"Rodriguez EM","year":"2012","journal-title":"Biol Res"},{"key":"B30","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1002\/ajmg.a.38592","volume":"176","author":"Ruggeri G","year":"2018","journal-title":"Am J Med Genet A"},{"key":"B31","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s40478-017-0438-4","volume":"5","author":"Saugier-Veber P","year":"2017","journal-title":"Acta Neuropathol Commun"},{"key":"B32","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1002\/ana.24964","volume":"81","author":"Shaheen R","year":"2017","journal-title":"Ann Neurol"},{"key":"B33","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1006\/prep.2001.1603","volume":"25","author":"Stols L","year":"2002","journal-title":"Protein Expr Purif"},{"key":"B34","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.celrep.2017.06.089","volume":"20","author":"Takagishi M","year":"2017","journal-title":"Cell Rep"},{"key":"B35","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/ng0893-421","volume":"4","author":"Van Camp G","year":"1993","journal-title":"Nat Genet"},{"key":"B36","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1177\/0300985811415708","volume":"49","author":"Vogel P","year":"2012","journal-title":"Vet Pathol"},{"key":"B37","volume":"2010","author":"Wallingford JB","year":"2010","journal-title":"Cold Spring Harb Protoc"},{"key":"B38","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ejmg.2017.12.002","volume":"61","author":"Wallis M","year":"2018","journal-title":"Eur J Med Genet"},{"key":"B39","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/nyas.13432","volume":"1405","author":"Yano T","year":"2017","journal-title":"Ann NY Acad Sci"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2019,7,19]],"date-time":"2019-07-19T16:21:50Z","timestamp":1563553310000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.E19-02-0091"}},"subtitle":[],"editor":[{"given":"Keith E.","family":"Mostov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,7,22]]},"references-count":39,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2019,7,22]]}},"alternative-id":["10.1091\/mbc.E19-02-0091"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e19-02-0091","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,22]]}}}