{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:56:55Z","timestamp":1788173815296,"version":"build-2803163510"},"reference-count":24,"publisher":"American Society for Cell Biology (ASCB)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2022,5,15]]},"abstract":"<jats:p>A novel cloud-based image processing and analysis platform is described that captures the intricate activity profiles revealed by GCaMP fluorescence recordings of intracellular calcium changes. It enables the discovery of molecules that correct 153 parameters that define the amyotrophic lateral sclerosis motor neuron disease phenotype.<\/jats:p>","DOI":"10.1091\/mbc.e21-10-0481","type":"journal-article","created":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T12:43:08Z","timestamp":1639572188000},"source":"Crossref","is-referenced-by-count":7,"title":["A multiparametric activity profiling platform for neuron disease phenotyping and drug screening"],"prefix":"10.1091","volume":"33","author":[{"given":"Bruno","family":"Boivin","sequence":"first","affiliation":[{"name":"F. M. Kirby Neurobiology Center, Boston Children\u2019s Hospital, Boston, MA 02115"},{"name":"Department of Neurobiology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasper C. D.","family":"Roet","sequence":"additional","affiliation":[{"name":"F. M. Kirby Neurobiology Center, Boston Children\u2019s Hospital, Boston, MA 02115"},{"name":"Department of Neurobiology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Huang","sequence":"additional","affiliation":[{"name":"F. M. Kirby Neurobiology Center, Boston Children\u2019s Hospital, Boston, MA 02115"},{"name":"Department of Neurobiology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle W.","family":"Karhohs","sequence":"additional","affiliation":[{"name":"Broad Institute of Harvard and MIT, Imaging Platform, Cambridge, MA 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad H.","family":"Rohban","sequence":"additional","affiliation":[{"name":"Broad Institute of Harvard and MIT, Imaging Platform, Cambridge, MA 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Sandoe","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole","family":"Wiskow","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rie","family":"Maeda","sequence":"additional","affiliation":[{"name":"F. M. Kirby Neurobiology Center, Boston Children\u2019s Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyssa","family":"Grantham","sequence":"additional","affiliation":[{"name":"F. M. Kirby Neurobiology Center, Boston Children\u2019s Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary K.","family":"Dornon","sequence":"additional","affiliation":[{"name":"F. M. Kirby Neurobiology Center, Boston Children\u2019s Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Shao","sequence":"additional","affiliation":[{"name":"F. M. Kirby Neurobiology Center, Boston Children\u2019s Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devlin","family":"Frost","sequence":"additional","affiliation":[{"name":"F. M. Kirby Neurobiology Center, Boston Children\u2019s Hospital, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dylan","family":"Baker","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Eggan","sequence":"additional","affiliation":[{"name":"Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne E.","family":"Carpenter","sequence":"additional","affiliation":[{"name":"Broad Institute of Harvard and MIT, Imaging Platform, Cambridge, MA 02142"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clifford J.","family":"Woolf","sequence":"additional","affiliation":[{"name":"F. M. Kirby Neurobiology Center, Boston Children\u2019s Hospital, Boston, MA 02115"},{"name":"Department of Neurobiology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cophys.2017.12.007"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-020-00117-w"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1038\/nature12354"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.09.017"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl355"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1113\/JP275498"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.38173"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109224"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1109\/MCSE.2007.55"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0158596"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.03.004"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.074138"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000011119"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2005970"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty281"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.08.009"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10483"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1126\/science.156.3777.976"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.11.037"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3584"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1109\/MCSE.2011.37"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1340-08.2008"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.03.019"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.21105\/joss.03021"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T06:48:35Z","timestamp":1661150915000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.E21-10-0481"}},"subtitle":[],"editor":[{"given":"Jennifer","family":"Lippincott-Schwartz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,5,15]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,5,15]]}},"alternative-id":["10.1091\/mbc.E21-10-0481"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e21-10-0481","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.06.22.449195","asserted-by":"object"}]},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,15]]},"article-number":"ar54"}}