{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:59:28Z","timestamp":1790801968694,"version":"4.1.0"},"reference-count":75,"publisher":"American Society for Cell Biology (ASCB)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2024,3,1]]},"abstract":"<jats:p>The tubulin-containing cytoskeleton of the human parasite Toxoplasma gondii includes several distinct structures: the conoid, formed of 14 ribbon-like tubulin polymers, and the array of 22 cortical microtubules (MTs) rooted in the apical polar ring. Here we analyze the structure of developing daughter parasites using both 3D-SIM and expansion microscopy. Cortical MTs and the conoid start to develop almost simultaneously, but from distinct precursors near the centrioles. Cortical MTs are initiated in a fixed sequence, starting around the periphery of a short arc that extends to become a complete circle. The conoid also develops from an open arc into a full circle, with a fixed spatial relationship to the centrioles. The patterning of the MT array starts from a \u201cblueprint\u201d with \u223cfive-fold symmetry, switching to 22-fold rotational symmetry in the final product, revealing a major structural rearrangement during daughter growth. The number of MT is essentially invariant in the wild-type array, but is perturbed by the loss of some structural components of the apical polar ring. This study provides insights into the development of tubulin-containing structures that diverge from conventional models, insights that are critical for understanding the evolutionary paths leading to construction and divergence of cytoskeletal frameworks.<\/jats:p>","DOI":"10.1091\/mbc.e23-11-0418","type":"journal-article","created":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T11:52:01Z","timestamp":1704282721000},"source":"Crossref","is-referenced-by-count":23,"title":["The initiation and early development of the tubulin-containing cytoskeleton in the human parasite\n                    <i>Toxoplasma gondii<\/i>"],"prefix":"10.1091","volume":"35","author":[{"given":"Luisa F. 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