{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T14:44:28Z","timestamp":1792853068606,"version":"4.5.0"},"reference-count":63,"publisher":"American Society for Cell Biology (ASCB)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2024,8,1]]},"abstract":"<jats:p>Bacterial microcompartments (BMCs) are widespread, protein-based organelles that regulate metabolism. The model for studying BMCs is the carboxysome, which facilitates carbon fixation in several autotrophic bacteria. Carboxysomes can be distinguished as type \u03b1 or \u03b2, which are structurally and phyletically distinct. We recently characterized the maintenance of carboxysome distribution (Mcd) systems responsible for spatially regulating \u03b1- and \u03b2-carboxysomes, consisting of the proteins McdA and McdB. McdA is an ATPase that drives carboxysome positioning, and McdB is the adaptor protein that directly interacts with carboxysomes to provide cargo specificity. The molecular features of McdB proteins that specify their interactions with carboxysomes, and whether these are similar between \u03b1- and \u03b2-carboxysomes, remain unknown. Here, we identify C-terminal motifs containing an invariant tryptophan necessary for \u03b1- and \u03b2-McdBs to associate with \u03b1- and \u03b2-carboxysomes, respectively. Substituting this tryptophan with other aromatic residues reveals corresponding gradients in the efficiency of carboxysome colocalization and positioning by McdB in vivo. Intriguingly, these gradients also correlate with the ability of McdB to form condensates in vitro. The results reveal a shared mechanism underlying McdB adaptor protein binding to carboxysomes, and potentially other BMCs. Our findings also implicate condensate formation as playing a key role in this association.<\/jats:p>","DOI":"10.1091\/mbc.e23-11-0443","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T12:06:45Z","timestamp":1719403605000},"source":"Crossref","is-referenced-by-count":10,"title":["An invariant C-terminal tryptophan in McdB mediates its interaction and positioning function with carboxysomes"],"prefix":"10.1091","volume":"35","author":[{"given":"Joseph L.","family":"Basalla","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Ghalmi","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Hoang","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel E.","family":"Dow","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6198-3245","authenticated-orcid":false,"given":"Anthony G.","family":"Vecchiarelli","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060910-095130"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.12.035"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.7"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.81362"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1021\/sb500226j"},{"key":"B7","first-page":"155","volume-title":"Circadian Rhythms: Methods and Protocols","author":"Clerico EM","year":"2007"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1101\/gr.849004"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2008122117"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjab010"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.77"},{"key":"B12","volume-title":"The Prokaryotes","author":"Dworkin M","year":"2006"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.59882"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.008"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2016.00044"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0223877"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1318"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/ell002"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1738"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.117.300064"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E21-03-0151"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba1269"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2774"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-47330-4"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-41-3-357"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2018.10"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2016.03.009"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.10.4648-4655.2006"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1186\/s13062-017-0197-y"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2014406118"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06044-0"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2012.05.002"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msz308"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.39723"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14708"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac240"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.8b06822"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2021.05.011"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32584-7"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e17-01-0069"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-020-0387-7"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1126\/science.1234264"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39019-x"},{"key":"B44","first-page":"300","volume":"23","author":"Rae BD","year":"2013","journal-title":"J Mol Microbiol Biotechnol"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02696-20"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1126\/science.1186090"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.66160"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1037"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2413"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.1817"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.18.00787"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.00885"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24126-4"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.1c00533"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(03)75005-X"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12017"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0880-5"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.06.006"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1038\/nsb1096-842"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1913"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.TM118.001191"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-021-00676-5"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T15:24:09Z","timestamp":1722266649000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.E23-11-0443"}},"subtitle":[],"editor":[{"given":"Erin","family":"Goley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,8,1]]},"references-count":63,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8,1]]}},"alternative-id":["10.1091\/mbc.E23-11-0443"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e23-11-0443","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.11.21.568049","asserted-by":"object"}]},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,1]]},"article-number":"ar107"}}