{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T04:20:01Z","timestamp":1783398001198,"version":"3.54.6"},"reference-count":56,"publisher":"American Society for Cell Biology (ASCB)","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2024,12,1]]},"abstract":"<jats:p> Microtubule (MT) regulation is essential for oocyte development. In Drosophila, MT stability, polarity, abundance, and orientation undergo dynamic changes across developmental stages. In our effort to identify novel microtubule-associated proteins that regulate MTs in the Drosophila ovary, we identified a previously uncharacterized gene, CG18190, which\u00a0encodes a novel MT end-binding (EB) protein, which we propose to name EB-SUN. We show that EB-SUN colocalizes with EB1 at growing MT plus-ends in Drosophila S2 cells. Tissue-specific and developmental expression profiles from Paralog Explorer reveal that EB-SUN is predominantly expressed in the ovary and early embryos, while EB1 is ubiquitously expressed. Furthermore, as early as oocyte determination, EB-SUN comets are highly concentrated in oocytes during oogenesis. EB-SUN knockout (KO) results in decreased MT density at the onset of mid-oogenesis (stage 7) and delays oocyte growth during late mid-oogenesis (stage 9). Combining EB-SUN KO with EB1 knockdown (KD) in germ cells significantly further reduces MT density at stage 7. Hatching assays of single protein depletion reveal distinct roles for EB-SUN and EB1 in early embryogenesis, likely due to differences in their\u00a0expression and binding partners. Notably, all eggs from EB-SUN KO\/EB1 KD females fail to hatch, suggesting partial redundancy between these proteins. <\/jats:p>","DOI":"10.1091\/mbc.e24-09-0402","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T15:50:42Z","timestamp":1730303442000},"source":"Crossref","is-referenced-by-count":3,"title":["EB-SUN, a new microtubule plus-end tracking protein in <i>Drosophila<\/i>"],"prefix":"10.1091","volume":"35","author":[{"given":"Sun K.","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611-3008"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen L.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Biology, Integrative Program for Biological and Genome Sciences, The University of North Carolina at Chapel Hill, Chapel Hill, NC 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