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Previous work showed that loss of F-actin blocks chloroplast division but not furrow ingression, suggesting that pharmacological perturbations can disorganize these events. In this study, we developed an imaging platform to screen natural compounds that perturb cell division while monitoring FtsZ and microtubules and identified 70 unique compounds. One compound, curcumin, has been proposed to bind to both FtsZ and tubulin proteins in bacteria and eukaryotes, respectively. In C. reinhardtii, where both targets coexist and are involved in cell division, curcumin at a specific dose range caused a severe disruption of the FtsZ ring in chloroplast while leaving the furrow-associated microtubule structures largely intact. Time-lapse imaging showed that loss of FtsZ and chloroplast division failure delayed the completion of furrowing but not the initiation, suggesting that the chloroplast division checkpoint proposed in other algae requires FtsZ or is absent altogether in C. reinhardtii.<\/jats:p>","DOI":"10.1091\/mbc.e24-09-0425","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T15:26:36Z","timestamp":1740756396000},"source":"Crossref","is-referenced-by-count":9,"title":["Imaging-based screen identifies novel natural compounds that perturb cell and chloroplast division in\n                    <i>Chlamydomonas reinhardtii<\/i>"],"prefix":"10.1091","volume":"36","author":[{"given":"Manuella R.","family":"Clark-Cotton","sequence":"first","affiliation":[{"name":"Department of Biology, Duke University, Durham, NC 27708"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-An","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of 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