{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:36:06Z","timestamp":1790217366245,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Cell Biology (ASCB)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2025,5,1]]},"abstract":"<jats:p> Amyloid \u03b2 (A\u03b2) inhibits hippocampal long-term potentiation (LTP; a form of synaptic plasticity thought to underly learning and memory) by inhibiting the stimulation-induced synaptic accumulation of the Ca<jats:sup>2+<\/jats:sup>\/calmodulin (CaM)-dependent protein kinase II (CaMKII). Notably, CaMKII inhibition rescues both CaMKII movement and LTP, indicating that CaMKII mediates both LTP and the A\u03b2-induced LTP impairment. Somewhat counterintuitively, we found here that overexpression of GFP-CaMKII also rescued the A\u03b2-induced impairment of CaMKII movement. For endogenous CaMKII, we confirmed that A\u03b2 indeed induced impairment of movement, and that previous results with live-imaging approaches were not due to A\u03b2-induced dissociation of the CaMKII intrabody. For exogenous GFP-CaMKII, the effect did not depend on the expression level and was thus likely caused by the N-terminal GFP label. Surprisingly, placing the GFP label instead at the C-terminus (near the association domain) still allowed CaMKII holoenzyme formation and still protected from the A\u03b2-induced impairment of CaMKII movement. Thus, while our method allows replacing endogenous CaMKII with similar amounts of GFP-CaMKII, our results provide a rare example for GFP-CaMKII not recapitulating the function of endogenous CaMKII. <\/jats:p>","DOI":"10.1091\/mbc.e24-10-0443","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T16:01:22Z","timestamp":1743004882000},"source":"Crossref","is-referenced-by-count":3,"title":["A\u03b2 impairs the LTP-related movement of endogenous CaMKII but not of exogenous GFP-CaMKII"],"prefix":"10.1091","volume":"36","author":[{"given":"Carolyn Nicole","family":"Brown","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan-Yi","family":"Chao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"},{"name":"Program in Neuroscience, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daphne","family":"Quang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"},{"name":"Program in Neuroscience, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole L.","family":"Rumian","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"},{"name":"Program in Neuroscience, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mason S.","family":"Kleinjan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Coultrap","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Ulrich","family":"Bayer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"},{"name":"Program in Neuroscience, University of Colorado Anschutz Medical Campus, Aurora, CO 80045"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-250407"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.08.034"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/35081080"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-024-01809-x"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.19.5598"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3116-05.2006"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.05.033"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.113982"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.103184"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104368"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23779-4"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe2300"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.03.041"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102299"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.01.005"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5352.870"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.05.068"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.04.017"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.482"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04439-7"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-0186(02)00050-5"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6448"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/nature07842"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1126\/science.2549638"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2108-13.2013"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.05.036"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201606210"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1038\/nm1782"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.162"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1126\/science.1378648"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1603-16.2016"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0085-05.2005"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5925"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06465-y"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.088617"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1038\/416535a"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-022-00624-2"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T16:35:09Z","timestamp":1746635709000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.E24-10-0443"}},"subtitle":[],"editor":[{"given":"Stephanie","family":"Gupton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,5,1]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5,1]]}},"alternative-id":["10.1091\/mbc.E24-10-0443"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e24-10-0443","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,1]]},"article-number":"ar60"}}