{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:15:47Z","timestamp":1791558947580,"version":"4.3.4"},"reference-count":66,"publisher":"American Society for Cell Biology (ASCB)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2025,7,1]]},"abstract":"<jats:p> Agrobacterium tumefaciens shifts from a free-living soil bacterium to a plant-invading state upon encountering the plant root microenvironment. The acid-induced two-component sensor system ChvG\u2013ChvI drives this shift and triggers a complex transcriptional program that promotes host invasion and survival against host immune defenses. Remarkably, ChvG\u2013ChvI is also activated under cell wall stress conditions, suggesting that the transcriptional response may have a broader function. Here, we find that blocking cell wall synthesis either genetically or chemically leads to ChvG\u2013ChvI activation. Mutations in key cell wall synthesis enzymes, such as penicillin-binding protein 1a and FtsW, suppress ChvG\u2013ChvI activation in cell wall stress inducing conditions, suggesting that providing structural integrity is a primary function of the ChvG\u2013ChvI regulon. Here, we investigated regulon components for this function. First, deletion of exoA, a gene required for production of the exopolysaccharide succinoglycan, confers resistance to multiple \u03b2-lactam antibiotics targeting different enzymes. Next, a class D \u03b2-lactamase is expressed that may contribute to the high level of \u03b2-lactam resistance in A. tumefaciens. Finally, outer membrane proteins are upregulated, suggesting that outer membrane remodeling may compensate for the accumulation of cell wall damage by providing structural integrity. Overall, we expand our understanding of mechanisms driving ChvG\u2013ChvI activation and \u03b2-lactam resistance in a bacterial plant pathogen. <\/jats:p>","DOI":"10.1091\/mbc.e24-12-0546","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T15:30:18Z","timestamp":1747323018000},"source":"Crossref","is-referenced-by-count":4,"title":["Activation of the ChvG\u2013ChvI pathway promotes survival during cell wall stress in <i>Agrobacterium tumefaciens<\/i>"],"prefix":"10.1091","volume":"36","author":[{"given":"Jacob M.","family":"Bouchier","sequence":"first","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO 65211"},{"name":"Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Knebel","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Amstutz","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Torrens","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Ume\u00e5 Centre for Microbial Research, Science for Life Laboratory, Ume\u00e5 University, Ume\u00e5 90187, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Santiago-Collazo","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carli","family":"McCurry","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra J.","family":"Weisberg","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Cava","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Ume\u00e5 Centre for Microbial Research, Science for Life Laboratory, Ume\u00e5 University, Ume\u00e5 90187, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7558-7155","authenticated-orcid":false,"given":"Pamela J. B.","family":"Brown","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1039\/D2SC00645F"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14823"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1011449"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00665-17"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01060-17"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1007\/BF00284690"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010776"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.5.2086-2091.1987"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092611-150039"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.20.6614-6625.1993"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00734-09"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06362.x"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.19.5183-5191.1998"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1007\/s002990050458"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.4161\/fly.19695"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12686"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2269-7_19"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/sdata.2017.151"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00333-22"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01140-09"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.21.7045-7055.1993"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-00799-3"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.08.027"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-120822-102714"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192439399"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01751-14"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-37"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14212"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805309105"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.19469"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1007\/s11240-005-0416-6"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-822312-3.00019-9"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.3390\/biom9110694"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1014699"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1021\/pr060636a"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.03.066"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13876"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00681-13"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192439499"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00313-12"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M909877199"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1038\/nature19331"},{"key":"B44","volume":"3","author":"Morton ER","year":"2012","journal-title":"Curr Protoc Microbiol"},{"key":"B45","volume":"1","author":"Morton ER","year":"2012","journal-title":"Curr Protoc Microbiol"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00225-24"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac993"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01676-09"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010465"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14119"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90418-P"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007726"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-00798-4"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2016.03.017"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0345-x"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2859-2863.2003"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00538-20"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06357-11"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba5256"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02346-21"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010274"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002938"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-7650-1"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01381-z"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01387-07"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T16:36:29Z","timestamp":1751387789000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.E24-12-0546"}},"subtitle":[],"editor":[{"given":"Ethan","family":"Garner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,7,1]]},"references-count":66,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7,1]]}},"alternative-id":["10.1091\/mbc.E24-12-0546"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e24-12-0546","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,1]]},"article-number":"ar84"}}