{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T11:45:10Z","timestamp":1790595910177,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Cell Biology (ASCB)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2025,8,1]]},"abstract":"<jats:p>Dermal aging is a complex process characterized by structural and functional changes in the extracellular matrix (ECM) and the resident cells, such as dermal fibroblasts. Fibronectin (FN) ECM is a crucial component of the dermis, yet its role in natural aging remains poorly understood. Here, we demonstrate a significant reduction in FN ECM in aged, otherwise healthy, mouse dermis. To explore whether aging dermal fibroblasts contribute to this decline in FN levels, we examined human dermal fibroblasts (HDF) from aged skin and observed that they produce lower extracellular FN densities in vitro than young HDFs. This age-associated deficiency correlates with reduced level of \u03b15-integrin, a cell-surface FN receptor essential for extracellular FN assembly. Knockdown of \u03b15-integrin (\u03b15KD) in young HDFs impairs FN ECM formation and induces phenotypes characteristic of cellular senescence. Interestingly, the proliferation defect in \u03b15KD fibroblasts is largely restored by cell-derived matrices\u00a0(CDMs) in vitro, highlighting the importance of ECM organization. These findings underscore the essential role of \u03b15-integrin in maintaining proper ECM formation, which in turn protects against fibroblast senescence during dermal aging.<\/jats:p>","DOI":"10.1091\/mbc.e25-02-0074","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T11:10:52Z","timestamp":1750849852000},"source":"Crossref","is-referenced-by-count":4,"title":["Dysregulation of extracellular fibronectin and \u03b15-integrin in dermal aging"],"prefix":"10.1091","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2561-6218","authenticated-orcid":false,"given":"Yaelim","family":"Lee","sequence":"first","affiliation":[{"name":"Mechanobiology Institute, National University of Singapore, Singapore 117411"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyu","family":"Shen","sequence":"additional","affiliation":[{"name":"Mechanobiology Institute, National University of Singapore, Singapore 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