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To more finely decrease and then restore transcriptional activity, we removed culture media serum and then added it back, respectively. Decreasing transcriptional activity decreases nuclear bleb formation, stability, and rupture, while returning transcriptional activity increases nuclear blebbing. These modulations of transcriptional activity did not alter the nuclear spring constant or actin confinement and contraction. Instead, we find that transcriptional activity regulates chromatin domain motion measured by mean square displacement (MSD) of chromatin domains labeled via transfected Cy3-dNTPs. Increasing chromatin domain motion using an established RAD51 inhibitor B02 resulted in increased nuclear blebbing. 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