{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:22:42Z","timestamp":1791354162201,"version":"4.3.1"},"reference-count":30,"publisher":"American Society for Cell Biology (ASCB)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2026,5,1]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Giardia intestinalis<\/jats:italic>\n                    is a globally prevalent cause of waterborne diarrheal disease, yet about 40% of its proteome remains functionally uncharacterized due to the lack of conserved homologous proteins and limited experimental validation of protein function. To begin addressing this gap, we created a large-scale subcellular localization resource by fluorescently tagging and imaging 608\n                    <jats:italic toggle=\"yes\">Giardia<\/jats:italic>\n                    proteins (12% of the proteome) expressed in live cells from native promoters. This dataset includes 240 hypothetical proteins, 215 domain-family proteins (including ankyrin repeat and NEK kinase families), 171 diplomonad- or\n                    <jats:italic toggle=\"yes\">Giardia<\/jats:italic>\n                    -specific proteins, 69 conserved eukaryotic proteins, and 77 proteins with known functions that were previously unlocalized. Imaging revealed localization to cytoskeletal and\n                    <jats:italic toggle=\"yes\">Giardia<\/jats:italic>\n                    -specific organelles (eight flagella, the ventral disk, and the median body), along with novel components of the plasma membrane and endomembrane systems. Integrating localization data with domain architecture, homology, and\n                    <jats:italic toggle=\"yes\">Giardia<\/jats:italic>\n                    -specific Gene Ontology terms, we produced a \u201clocalization-informed\u201d gene annotation with a standardized, structured nomenclature. This resource provides the largest experimentally validated functional annotation of the\n                    <jats:italic toggle=\"yes\">Giardia<\/jats:italic>\n                    proteome to date, linking predicted gene models to cellular structures, creating testable hypotheses for protein function and establishing a durable framework for future studies of cell biology, pathogenesis, and eukaryotic evolution in this deeply divergent diplomonad lineage.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e25-12-0590","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T16:55:52Z","timestamp":1774457752000},"source":"Crossref","is-referenced-by-count":2,"title":["Breaking the annotation barrier: An initial subcellular localization atlas of\n                    <i>Giardia<\/i>\n                    \u2019s hypothetical and conserved hypothetical proteins provides a resource for functional discovery"],"prefix":"10.1091","volume":"37","author":[{"given":"Kari D.","family":"Hagen","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, CA 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander J.","family":"Zerkle","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, CA 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satvik","family":"Arani","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, CA 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Chase","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, CA 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