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In this study, we identified several genes known to regulate aneuploidy and mitosis, and subsequently focused on\n                    <jats:italic toggle=\"yes\">PRR14L<\/jats:italic>\n                    , a ubiquitously expressed gene previously implicated in chronic myelomonocytic leukemia (CMML). Proximity labeling of PRR14L using TurboID revealed several cell division proteins, including the PP2A-B56 phosphatase complex and the spindle assembly factor TACC3, as PRR14L-interacting proteins. Loss of PRR14L prolongs SAC-dependent mitotic arrest in response to microtubule depolymerization but, paradoxically, leads to catastrophic mitotic errors upon SAC abrogation by MPS1 inhibitors. A model derived from our findings provides a rationale for exploiting MPS1 inhibition as a potential vulnerability in cancers containing either PRR14L loss-of-function mutations or FGFR\u2212TACC3 fusions.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e25-12-0634","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T19:01:28Z","timestamp":1779303688000},"source":"Crossref","is-referenced-by-count":0,"title":["The scaffold protein PRR14L is linked to mitotic fidelity and sensitivity to MPS1 inhibition"],"prefix":"10.1091","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9387-0878","authenticated-orcid":false,"given":"Albert Z.","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205"},{"name":"Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshay","family":"Narkar","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keming","family":"Li","sequence":"additional","affiliation":[{"name":"Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Bertomeu","sequence":"additional","affiliation":[{"name":"The ChemoGenix CRISPR screening facility of the Institute for Research in Immunology and Cancer, University of Montreal, 2950 Chemin de Polytechnique, Montr\u00e9al, QC H3T 1J4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blake A.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmin","family":"Coulombe-Huntington","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, McGill University, Montreal, QC H3A 0E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Tyers","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada"},{"name":"Program in Molecular Medicine, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, MD 21205"},{"name":"Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore"},{"name":"Department of Biological Sciences, National University of Singapore, Singapore 117558, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-02085-6"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1382"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-023-00711-y"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029611"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25432"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00302-17"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.15"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4201"},{"key":"e_1_3_5_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-1337"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gore.2015.06.005"},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0340-5"},{"key":"e_1_3_5_13_1","first-page":"1","article-title":"Identification of B56 \u03b1, B56 \u03b3, and BAP1 as PRR14L binding partners","author":"Chase A","year":"2023","unstructured":"Chase A, Tarragona GC, Lin F, Yapp S, Score J, Bryant C, Cross NCP (2023). 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