{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:57:05Z","timestamp":1791478625113,"version":"4.3.4"},"reference-count":0,"publisher":"American Society for Cell Biology (ASCB)","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"abstract":"<jats:p>\n                    Septins are cytoskeletal proteins that regulate cytokinesis in fungi and animals, yet their functions in choanoflagellates\u2014the closest living relatives of animals\u2014have remained unknown.\n                    <jats:italic toggle=\"yes\">Salpingoeca rosetta<\/jats:italic>\n                    , a choanoflagellate that switches between unicellular and multicellular forms, encodes four septins that are closely related to animal and fungal septins. Disruption of three of these septins altered cell size and impaired multicellular rosette development, with two of these three also required for normal rosette integrity. Characterization of\n                    <jats:italic toggle=\"yes\">Sros_septA<\/jats:italic>\n                    , which showed the strongest phenotype, revealed a role in cytokinesis: mutant cells underwent furrow ingression but frequently failed to complete cell division, resulting in enlarged, multinucleated cells. Cell size and rates of cytokinesis failure and multinucleation were elevated in uninduced\n                    <jats:italic toggle=\"yes\">Sros_septA<\/jats:italic>\n                    mutant cells and increased further upon rosette induction, revealing a heightened requirement for\n                    <jats:italic toggle=\"yes\">Sros_septA<\/jats:italic>\n                    in cytokinesis during multicellular development in\n                    <jats:italic toggle=\"yes\">S. rosetta<\/jats:italic>\n                    . Consistent with these cytokinesis defects, endogenously tagged\n                    <jats:italic toggle=\"yes\">Sros<\/jats:italic>\n                    _SeptA dynamically redistributed from the basal pole in interphase cells to the cleavage furrow and nascent intercellular bridge during cell division. These findings identify roles for septins in cytokinesis and multicellular development in\n                    <jats:italic toggle=\"yes\">S. rosetta<\/jats:italic>\n                    and offer a framework for exploring the evolution of cytokinetic regulation alongside the emergence of multicellularity in the animal stem lineage.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e26-03-0141","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:30:31Z","timestamp":1791477031000},"source":"Crossref","is-referenced-by-count":0,"title":["Septins regulate cytokinesis and multicellular development in the closest living relatives of animals"],"prefix":"10.1091","author":[{"given":"Michael","family":"Carver","sequence":"first","affiliation":[{"name":"University of California, Berkeley"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"King","sequence":"additional","affiliation":[{"name":"University of California, Berkeley"},{"name":"Howard Hughes Medical Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","published-online":{"date-parts":[[2026,10,8]]},"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:30:32Z","timestamp":1791477032000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.E26-03-0141"}},"subtitle":[],"editor":[{"given":"Masayuki","family":"Onishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,10,8]]},"references-count":0,"alternative-id":["10.1091\/mbc.E26-03-0141"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e26-03-0141","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,8]]},"article-number":"mbc.E26-03-0141"}}