{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T15:35:15Z","timestamp":1793374515000,"version":"4.5.0"},"reference-count":79,"publisher":"American Society for Cell Biology (ASCB)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2026,11,1]]},"abstract":"<jats:p>\n                    Septins are a family of GTP-binding proteins found in many eukaryotic lineages. Although highly conserved throughout many eukaryotes, their functions vary across species. In\n                    <jats:italic toggle=\"yes\">Aspergillus fumigatus<\/jats:italic>\n                    , the etiological agent of invasive aspergillosis, septins participate in a variety of processes, including conidiation, septation, and responses to cell wall stress. Previous studies determined that the \u0394\n                    <jats:italic toggle=\"yes\">aspB<\/jats:italic>\n                    septin deletion strain had a greater sensitivity to anti-cell wall drugs, especially the echinocandins, yet the mechanisms behind this augmented sensitivity are unknown. We performed cell viability staining of the septin deletion strains after caspofungin exposure and found that the \u0394\n                    <jats:italic toggle=\"yes\">aspA<\/jats:italic>\n                    , \u0394\n                    <jats:italic toggle=\"yes\">aspB<\/jats:italic>\n                    , and \u0394\n                    <jats:italic toggle=\"yes\">aspC<\/jats:italic>\n                    strains had significantly lower cell viability. Concomitant with the reduced viability, these septin deletion strains are more susceptible to caspofungin on solid media. These results indicate that the septin cytoskeleton is important for\n                    <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                    survival in the presence of caspofungin. Due to the potential for improved therapeutic outcome, we followed up using a neutropenic murine model of invasive aspergillosis. Animals infected with the \u0394\n                    <jats:italic toggle=\"yes\">aspB<\/jats:italic>\n                    strain and treated with caspofungin showed improved survival compared with the animals infected with the \u0394\n                    <jats:italic toggle=\"yes\">akuB<\/jats:italic>\n                    <jats:sup>KU80<\/jats:sup>\n                    wild-type or complemented strains. Additionally, histological analysis showed reduced fungal burden in the \u0394\n                    <jats:italic toggle=\"yes\">aspB<\/jats:italic>\n                    -infected, caspofungin-treated group. To gain insight into how AspB coordinates fungal response to caspofungin, we used proteomics and identified proteins involved in the septin-dependent response to caspofungin, including four candidate interactors involved in cell wall stress response. Deletion of these candidate genes resulted in increased susceptibility to caspofungin and moderately reduced viability post-drug exposure. Taken together, these data suggest that septin AspB contributes to the fungistatic response to caspofungin.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e26-03-0151","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:52:39Z","timestamp":1789577559000},"source":"Crossref","is-referenced-by-count":0,"title":["Deletion of core septin gene\n                    <i>aspB<\/i>\n                    in\n                    <i>Aspergillus fumigatus<\/i>\n                    results in fungicidal activity of caspofungin"],"prefix":"10.1091","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6390-5325","authenticated-orcid":false,"given":"Rebecca Jean","family":"Busch","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Virginia Tech, Blacksburg 24061, Virginia"},{"name":"Center for Emerging, Zoonotic, and Arthropod-borne Pathogens, Virginia Tech, Blacksburg, Virginia 24061"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carson","family":"Doty","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Southern Illinois University-Carbondale, Carbondale, Illinois 62901"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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