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PES-8 contains a predicted extracellular zona pellucida-like domain and an unstructured cytoplasmic tail, suggesting dual roles in extracellular and cytoplasmic signaling. PES-8 localizes to the plasma membrane of the spermatheca, the spermathecal-uterine valve, and the uterus. Functional analysis shows that PES-8 is essential for spermathecal function; its loss disrupts actomyosin fiber alignment, FLN-1\/filamin localization, apical junction organization, and Ca\u00b2\u207a signaling, preventing oocyte transit. These findings identify PES-8 as a key regulator of cytoskeletal organization and calcium-mediated contractility in the\n                    <jats:italic toggle=\"yes\">C. elegans<\/jats:italic>\n                    spermatheca.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e26-04-0163","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T19:17:53Z","timestamp":1785957473000},"source":"Crossref","is-referenced-by-count":2,"title":["PES-8, a ZP-like protein, is required for cytoskeletal organization and contractility in the\n                    <i>Caenorhabditis elegans<\/i>\n                    spermatheca"],"prefix":"10.1091","volume":"37","author":[{"given":"Fereshteh","family":"Sadeghian","sequence":"first","affiliation":[{"name":"Bioengineering, Northeastern University, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8129-1326","authenticated-orcid":false,"given":"Erin J","family":"Cram","sequence":"additional","affiliation":[{"name":"Biology, Northeastern University, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01146"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-4186-7_12"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2018.02.007"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80853-9"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.024"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E18-10-0633"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.02-01-0008"},{"key":"e_1_3_4_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1201-1060"},{"key":"e_1_3_4_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008644"},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki396"},{"key":"e_1_3_4_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009188"},{"key":"e_1_3_4_14_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.118.301752"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.141.1.297"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/10.6.673"},{"key":"e_1_3_4_17_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.078964"},{"key":"e_1_3_4_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.197384"},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.91079"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzh013"},{"key":"e_1_3_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.12.069"},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2021.100748"},{"key":"e_1_3_4_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ado4167"},{"key":"e_1_3_4_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyab014"},{"key":"e_1_3_4_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.09.041"},{"key":"e_1_3_4_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21341"},{"key":"e_1_3_4_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyae056"},{"key":"e_1_3_4_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780199637393.001.0001"},{"key":"e_1_3_4_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11182777"},{"key":"e_1_3_4_30_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.74.082803.133039"},{"key":"e_1_3_4_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb802"},{"key":"e_1_3_4_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_4_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2014.02.036"},{"key":"e_1_3_4_34_1","article-title":"Regulation of actin dynamics in the C. elegans somatic gonad","volume":"7","author":"Kelley CA","year":"2019","unstructured":"Kelley CA, Cram EJ (2019). 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