{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:43:07Z","timestamp":1792690987784,"version":"4.5.0"},"reference-count":0,"publisher":"American Society for Cell Biology (ASCB)","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"abstract":"<jats:p>\n                    Cilia are highly conserved organelles that use hundreds of unique proteins to drive extracellular motility and serve as signaling hubs for eukaryotic cells. The Ciliogenesis and Planar Polarity Effector (CPLANE) protein complex controls basal body docking during early ciliogenesis and recruits the intraflagellar transport (IFT) proteins to the base of growing cilia. Leveraging recent advantages in deep learning neural networks and high-performance computing, we predict the structure of JBTS17, the largest CPLANE subunit, and the tertiary structure of the complete CPLANE complex, revealing direct contacts between JBTS17 and three other CPLANE members. We then used this model for an\n                    <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                    protein-protein interaction screen that identified several novel JBTS17 interactors. Experiments in\n                    <jats:italic toggle=\"yes\">Xenopus<\/jats:italic>\n                    confirmed a functional link\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    between Jbts17 and the Spinocerebellar ataxia-associated protein TTBK2. The data provide new insights into mechanisms of CPLANE function during ciliogenesis and demonstrate the power of modeling protein complexes for the discovery of physical and functional protein interactions in cell homeostasis and disease.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e26-05-0212","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:25:40Z","timestamp":1792689940000},"source":"Crossref","is-referenced-by-count":0,"title":["Predicted structure of the complete CPLANE complex reveals novel interactors and mechanisms of Jbts17 during ciliogenesis"],"prefix":"10.1091","author":[{"given":"Tynan","family":"Gardner","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USAs"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Prolomova","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USAs"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanjae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USAs"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward M.","family":"Marcotte","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USAs"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John B.","family":"Wallingford","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USAs"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","published-online":{"date-parts":[[2026,10,22]]},"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:25:41Z","timestamp":1792689941000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.E26-05-0212"}},"subtitle":[],"editor":[{"given":"Gregory J","family":"Pazour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,10,22]]},"references-count":0,"alternative-id":["10.1091\/mbc.E26-05-0212"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e26-05-0212","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,22]]},"article-number":"mbc.E26-05-0212"}}