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The nascent myotubes of the pupal\n                    <jats:italic toggle=\"yes\">Drosophila<\/jats:italic>\n                    testis provide an excellent model for N-cadherin mediated mesenchymal migration. We combined a proximity proteomics dataset of adherens junction proteins in mammalian epithelial cells with genome-wide shRNA libraries knocking down\n                    <jats:italic toggle=\"yes\">Drosophila<\/jats:italic>\n                    genes to begin to define the subset of junctional proteins important in mesenchymal migration. While N-cadherin is predominant, E-cadherin plays a supporting role. Surprisingly, several proteins with key roles in epithelial morphogenesis, including Afadin's homolog Canoe, ZO-1\u2032s homolog Polychaetoid, and Par3\u2019s homolog Bazooka, play at most modest roles. A total of 22 genes with diverse cell biological roles had strong to moderate defects in testis morphogenesis. These will provide a community resource. We followed up on two. The kinase Par-1 is important for migration and gap closure, with knockdown phenotypes paralleling those of myosin. The Rab GAP RN-tre does not have roles until after migration and works in parallel with N-cadherin during testis spiralization.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e26-05-0234","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T16:37:05Z","timestamp":1785343025000},"source":"Crossref","is-referenced-by-count":0,"title":["A screen for adherens junction proteins regulating collective cell migration and testis morphogenesis reveals important roles for the Rab GAP RN-tre and the kinase Par-1"],"prefix":"10.1091","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4478-8202","authenticated-orcid":false,"given":"Sarah E.","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill, CB#3280, Chapel Hill, NC 27599-3280"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siobhan 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