{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T15:35:15Z","timestamp":1793374515633,"version":"4.5.0"},"reference-count":118,"publisher":"American Society for Cell Biology (ASCB)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2026,11,1]]},"abstract":"<jats:p>\n                    Dynactin, a complex essential for cytoplasmic dynein function, contains an Arp1 mini-filament with a pointed-end sub-complex including Arp11 and a shoulder sub-complex including p50 and p150. We recently identified in\n                    <jats:italic toggle=\"yes\">Aspergillus nidulans<\/jats:italic>\n                    a vezatin homologue, VezA, that enhances dynactin assembly, but VezA does not co-localize with dynactin, suggesting a transient interaction. It was found that overexpression of p50 separates the shoulder from the mini-filament and causes late-onset neurodegeneration in mice, but its cellular effects in the context of VezA need to be studied. Here, we surprisingly found that p50 overexpression in\n                    <jats:italic toggle=\"yes\">A. nidulans<\/jats:italic>\n                    causes Arp11, p50 (but not p150), and VezA to form co-localized clusters whose presence depends on Arp1. Formation of the VezA cluster also depends on Arp11, and VezA significantly enhances the intensity of Arp11-GFP clusters but not that of p50-GFP clusters. Moreover, an evolutionarily conserved region of p50 (aa17-25) forming a beta-sheet structure with Arp1, as revealed in previous cryo-EM studies, is critical for the formation of Arp11, p50, and VezA clusters. These results suggest that excess p50 induces cluster formation driven by p50-Arp1 interactions, and that this assembly intermediate lacking the intact shoulder traps VezA, which participates in the Arp11-Arp1-p50 interactions.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e26-06-0275","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:42:10Z","timestamp":1790786530000},"source":"Crossref","is-referenced-by-count":0,"title":["Excess p50 induces Arp1-dependent dynactin clusters containing the assembly factor VezA"],"prefix":"10.1091","volume":"37","author":[{"given":"Jun","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences- F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongde","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences- F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences- F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00848.x"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_4_4_1","unstructured":"Allen P Neiswender H Lu W Lakonishok M Veeranan-Karmegam R Pride J Gelfand VI Gonsalvez GB (2026). An in vivo examination of Dynein-Cargo complex formation. bioRxiv: 2026.08.02.742322"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.079103"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.200900015"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110598"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.2.469"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05186-y"},{"key":"e_1_3_4_10_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e14-03-0842"},{"key":"e_1_3_4_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2996"},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-120420-104341"},{"key":"e_1_3_4_13_1","article-title":"Structural basis of dynein interaction with diverse activating adaptors","author":"d'Amico EA","year":"2026","unstructured":"d'Amico EA, Chaaban S, Abid Ali F, Michalski L, Carter AP (2026). Structural basis of dynein interaction with diverse activating adaptors. bioRxiv: 2026.04.25.720804.","journal-title":"bioRxiv"},{"key":"e_1_3_4_14_1","first-page":"221","article-title":"Conformational transitions of the Spindly adaptor underlie its interaction with Dynein and Dynactin","author":"d'Amico EA","year":"2022","unstructured":"d'Amico EA, Ud Din Ahmad M, Cmentowski V, Girbig M, M\u00fcller F, Wohlgemuth S, Brockmeyer A, Maffini S, Janning P, Vetter IR, et al. (2022). Conformational transitions of the Spindly adaptor underlie its interaction with Dynein and Dynactin. J Cell Biol 221.","journal-title":"J Cell Biol"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3084-11.2012"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E25-04-0186"},{"key":"e_1_3_4_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2999"},{"key":"e_1_3_4_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.132.4.617"},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.2.307"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201610108"},{"key":"e_1_3_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)80122-0"},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-026-01887-y"},{"key":"e_1_3_4_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.6.1639"},{"key":"e_1_3_4_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03592-2"},{"key":"e_1_3_4_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00412-018-0680-y"},{"key":"e_1_3_4_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-018-0027-7"},{"key":"e_1_3_4_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1083129"},{"key":"e_1_3_4_28_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-08-0695"},{"key":"e_1_3_4_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00200-7"},{"key":"e_1_3_4_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.09.053"},{"key":"e_1_3_4_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1620141114"},{"key":"e_1_3_4_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew047"},{"key":"e_1_3_4_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/351579a0"},{"key":"e_1_3_4_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2014.07.010"},{"key":"e_1_3_4_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.48.28806"},{"key":"e_1_3_4_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.4.1023"},{"key":"e_1_3_4_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.7.4834"},{"key":"e_1_3_4_38_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E19-06-0313"},{"key":"e_1_3_4_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.22.6020"},{"key":"e_1_3_4_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00696-7"},{"key":"e_1_3_4_41_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020106164"},{"key":"e_1_3_4_42_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200209022"},{"key":"e_1_3_4_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601119"},{"key":"e_1_3_4_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2591"},{"key":"e_1_3_4_45_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-150476"},{"key":"e_1_3_4_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00702.x"},{"key":"e_1_3_4_47_1","first-page":"8","article-title":"Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunction","author":"Marzo MG","year":"2019","unstructured":"Marzo MG, Griswold JM, Ruff KM, Buchmeier RE, Fees CP, Markus SM (2019). Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunction. Elife 8.","journal-title":"Elife"},{"key":"e_1_3_4_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1254198"},{"key":"e_1_3_4_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700003200"},{"key":"e_1_3_4_50_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01421.x"},{"key":"e_1_3_4_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00710.x"},{"key":"e_1_3_4_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(98)01389-0"},{"key":"e_1_3_4_53_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0935-13.2013"},{"key":"e_1_3_4_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/gtc.12936"},{"key":"e_1_3_4_55_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.105.052563"},{"key":"e_1_3_4_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2025.104018"},{"key":"e_1_3_4_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41466-5"},{"key":"e_1_3_4_58_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.227132"},{"key":"e_1_3_4_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2127"},{"key":"e_1_3_4_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dev022"},{"key":"e_1_3_4_61_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2522636123"},{"key":"e_1_3_4_62_1","first-page":"e54234","article-title":"ALS-linked KIF5A \u0394Exon27 mutant causes neuronal toxicity through gain-of-function","author":"Pant DC","year":"2022","unstructured":"Pant DC, Parameswaran J, Rao L, Loss I, Chilukuri G, Parlato R, Shi L, Glass JD, Bassell GJ, Koch P, et al. (2022). ALS-linked KIF5A \u0394Exon27 mutant causes neuronal toxicity through gain-of-function. EMBO Rep:e54234.","journal-title":"EMBO Rep"},{"key":"e_1_3_4_63_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-03-0254"},{"key":"e_1_3_4_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.07.017"},{"key":"e_1_3_4_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2958-1_1"},{"key":"e_1_3_4_66_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.412403"},{"key":"e_1_3_4_67_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.004000"},{"key":"e_1_3_4_68_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E20-03-0166"},{"key":"e_1_3_4_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00062-09"},{"key":"e_1_3_4_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-026-01877-0"},{"key":"e_1_3_4_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-026-10153-y"},{"key":"e_1_3_4_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0004-3"},{"key":"e_1_3_4_73_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00068-17"},{"key":"e_1_3_4_74_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202508066"},{"key":"e_1_3_4_75_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E20-08-0559"},{"key":"e_1_3_4_76_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202310022"},{"key":"e_1_3_4_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2010.02.008"},{"key":"e_1_3_4_78_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.126.2.403"},{"key":"e_1_3_4_79_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201488792"},{"key":"e_1_3_4_80_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.20.012103.094623"},{"key":"e_1_3_4_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-59606-4"},{"key":"e_1_3_4_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00013-7"},{"key":"e_1_3_4_83_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adk8544"},{"key":"e_1_3_4_84_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21049"},{"key":"e_1_3_4_85_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01066"},{"key":"e_1_3_4_86_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.120.303499"},{"key":"e_1_3_4_87_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e12-03-0210"},{"key":"e_1_3_4_88_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.FUNK-0034-2016"},{"key":"e_1_3_4_89_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e13-09-0557"},{"key":"e_1_3_4_90_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2010.01451.x"},{"key":"e_1_3_4_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.04.013"},{"key":"e_1_3_4_92_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.70496"},{"key":"e_1_3_4_93_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25462"},{"key":"e_1_3_4_94_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa4080"},{"key":"e_1_3_4_95_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.12.4107"},{"key":"e_1_3_4_96_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.10.1437"},{"key":"e_1_3_4_97_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.131.6.1507"},{"key":"e_1_3_4_98_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44319-025-00666-4"},{"key":"e_1_3_4_99_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04233-2"},{"key":"e_1_3_4_100_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90097-8"},{"key":"e_1_3_4_101_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1634"},{"key":"e_1_3_4_102_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2017.10.024"},{"key":"e_1_3_4_103_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.6.2100"},{"key":"e_1_3_4_104_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00488-7"},{"key":"e_1_3_4_105_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.598952"},{"key":"e_1_3_4_106_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E15-08-0602"},{"key":"e_1_3_4_107_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2011.01312.x"},{"key":"e_1_3_4_108_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2013.30"},{"key":"e_1_3_4_109_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e12-07-0496"},{"key":"e_1_3_4_110_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-08-0516"},{"key":"e_1_3_4_111_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201308009"},{"key":"e_1_3_4_112_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114943"},{"key":"e_1_3_4_113_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00748.x"},{"key":"e_1_3_4_114_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201011022"},{"key":"e_1_3_4_115_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.075259"},{"key":"e_1_3_4_116_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.05.025"},{"key":"e_1_3_4_117_1","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12917"},{"key":"e_1_3_4_118_1","article-title":"The Yun\/Prohibitin complex regulates adult Drosophila intestinal stem cell proliferation through the transcription factor E2F1","volume":"119","author":"Zhao H","year":"2022","unstructured":"Zhao H, Shi L, Li Z, Kong R, Ren X, Ma R, Jia L, Ma M, Lu S, Xu R, et al. (2022). The Yun\/Prohibitin complex regulates adult Drosophila intestinal stem cell proliferation through the transcription factor E2F1. Proc Natl Acad Sci USA 119.","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_119_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.106.069013"}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T14:56:28Z","timestamp":1793372188000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.E26-06-0275"}},"subtitle":[],"editor":[{"given":"Claire","family":"Walczak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,11,1]]},"references-count":118,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11,1]]}},"alternative-id":["10.1091\/mbc.E26-06-0275"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e26-06-0275","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,11,1]]},"article-number":"ar110"}}