{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:58:39Z","timestamp":1785855519977,"version":"3.56.0"},"reference-count":50,"publisher":"American Society for Cell Biology (ASCB)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:p>\n                    The light-responsive outer segment of rod photoreceptors is composed of two distinct membrane subdomains: discs and the plasma membrane. We investigate how the disk protein peripherin-2 is engaged in CNG channel delivery to the outer segment. Instead of forming outer segments, peripherin-2 knockout (\n                    <jats:italic toggle=\"yes\">\n                      Rds\n                      <jats:sup>-\/-<\/jats:sup>\n                    <\/jats:italic>\n                    ) photoreceptors release ciliary ectosomes, in which CNG channel levels are markedly reduced relative to other outer segment proteins. This is intriguing as downregulation of the CNG channel is not a general feature of degenerative mouse models with dysmorphic outer segments. Overexpression of the \u03b21-subunit of CNG in\n                    <jats:italic toggle=\"yes\">\n                      Rds\n                      <jats:sup>-\/-<\/jats:sup>\n                    <\/jats:italic>\n                    rods reveals that the majority is trapped in intracellular membranes, but is restored to the outer segment by co-expressing peripherin-2. We test peripherin-2 chimeras containing either the N-terminus, tetraspanin core, or C-terminus and find that the tetraspanin domain is sufficient to localize CNG\u03b21 to the outer segment. We further show that the membrane remodeling function of the tetraspanin domain facilitates this process by the redundant action of the tetraspanin domain from ROM1 and the reemergence of the endogenous CNG channel in aged\n                    <jats:italic toggle=\"yes\">\n                      Rds\n                      <jats:sup>-\/-<\/jats:sup>\n                    <\/jats:italic>\n                    rods that have produced ciliary membrane protrusions.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e26-06-0276","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T17:36:43Z","timestamp":1782927403000},"source":"Crossref","is-referenced-by-count":0,"title":["The tetraspanin disc proteins, peripherin-2 and ROM1, facilitate CNG channel localization to the rod outer segment"],"prefix":"10.1091","volume":"37","author":[{"given":"Molly T.","family":"Thorson","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, 48105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie E.","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, 48105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junseo","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, 48105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Y.","family":"Mart\u00ednez-M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, 48105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Ball","sequence":"additional","affiliation":[{"name":"Center for Vision Research, SUNY Upstate Medical University, Syracuse, NY, 13202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason R.","family":"Willer","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, 48105"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Spencer","sequence":"additional","affiliation":[{"name":"Center for Vision Research, SUNY Upstate Medical University, Syracuse, NY, 13202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jillian N.","family":"Pearring","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Science, University of Michigan, Ann Arbor, MI, 48105"},{"name":"Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 48105"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00023-8"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202000746"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2022.07.001"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1509285113"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-17785"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-18516"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/362764a0"},{"key":"e_1_3_4_9_1","first-page":"832","article-title":"Some cytological and initial biochemical observations on photoreceptors in retinas of rds mice","volume":"24","author":"Cohen AI","year":"1983","unstructured":"Cohen AI (1983). 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