{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:03:15Z","timestamp":1790787795713,"version":"4.1.0"},"reference-count":0,"publisher":"American Society for Cell Biology (ASCB)","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"abstract":"<jats:p>\n                    Z-discs define sarcomere boundaries and anchor actin filaments in muscle, yet many structural components remain uncharacterized. In this paper, we identify three new members of the Alp\/Enigma family in\n                    <jats:italic toggle=\"yes\">Drosophila<\/jats:italic>\n                    , including Uchmaz (CG42319), a PDZ domain-containing protein, that we characterize in detail. We used CRISPR\/Cas9 to generate multiple\n                    <jats:italic toggle=\"yes\">uchmaz<\/jats:italic>\n                    knockout alleles, which are viable but result in a flightless phenotype. Mutants exhibit disrupted sarcomere organization in the indirect flight muscles (IFMs), including fragmented and misaligned Z-discs and reduced accumulation of \u03b1-Actinin and other Z-disc markers. We find that Uchmaz localizes to the Z-disc, associates with \u03b1-Actinin, and is dependent upon \u03b1-Actinin for its localization to the Z-disc. These results identify Uchmaz as a component of IFM myofibril architecture required for muscle function. Since a human ortholog of\n                    <jats:italic toggle=\"yes\">uchmaz<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">PDLIM2<\/jats:italic>\n                    , is associated with severe muscle dysfunction in muscular dystrophies and myofibrillar myopathy, our studies provide insight into conserved mechanisms of Z-disc assembly and its relevance to human muscle disease.\n                  <\/jats:p>","DOI":"10.1091\/mbc.e26-06-0293","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:42:10Z","timestamp":1790786530000},"source":"Crossref","is-referenced-by-count":0,"title":["A novel Drosophila ALP\/Enigma protein binds to Alpha-actinin to promote sarcomere assembly"],"prefix":"10.1091","author":[{"given":"Ebru","family":"Robinson","sequence":"first","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA 92182."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiannis","family":"Alvarado","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA 92182."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Mason","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA 92182."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shayla","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA 92182."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard M.","family":"Cripps","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA 92182."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","published-online":{"date-parts":[[2026,9,30]]},"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:42:12Z","timestamp":1790786532000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.E26-06-0293"}},"subtitle":[],"editor":[{"given":"Elizabeth","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":0,"alternative-id":["10.1091\/mbc.E26-06-0293"],"URL":"https:\/\/doi.org\/10.1091\/mbc.e26-06-0293","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"article-number":"mbc.E26-06-0293"}}