{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T04:00:30Z","timestamp":1771560030493,"version":"3.50.1"},"reference-count":69,"publisher":"American Society for Cell Biology (ASCB)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:p>We have cloned the UNI3 gene inChlamydomonas and find that it encodes a new member of the tubulin superfamily. Although Uni3p shares significant sequence identity with \u03b1-, \u03b2-, and \u03b3-tubulins, there is a region of Uni3p that has no similarity to tubulins or other known proteins. Mutantuni3\u20131 cells assemble zero, one, or two flagella. Pedigree analysis suggests that flagellar number inuni3\u20131 cells is a function of the age of the cell. The uniflagellate uni3\u20131 cells show a positional phenotype; the basal body opposite the eyespot templates the single flagellum. A percentage of uni3\u20131 cells also fail to orient the cleavage furrow properly, and basal bodies have been implicated in the placement of cleavage furrows in Chlamydomonas. Finally when uni3\u20131 cells are observed by electron microscopy, doublet rather than triplet microtubules are observed at the proximal end of the basal bodies. We propose that the Uni3 tubulin is involved in both the function and cell cycle-dependent maturation of basal bodies\/centrioles.<\/jats:p>","DOI":"10.1091\/mbc.9.6.1293","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T23:13:12Z","timestamp":1372806792000},"page":"1293-1308","source":"Crossref","is-referenced-by-count":196,"title":["The<i>UNI3<\/i>Gene Is Required for Assembly of Basal Bodies of<i>Chlamydomonas<\/i>and Encodes \u03b4-Tubulin, a New Member of the Tubulin Superfamily"],"prefix":"10.1091","volume":"9","author":[{"given":"Susan K.","family":"Dutcher","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, Colorado 80309-0347"}]},{"given":"Emanuel C.","family":"Trabuco","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, Colorado 80309-0347"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"Altschul S.F.","year":"1990","journal-title":"J. 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