{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T20:44:47Z","timestamp":1789591487365,"version":"build-2803163510"},"reference-count":83,"publisher":"American Society for Cell Biology (ASCB)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["MBoC"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:p>Autocrine motility factor receptor (AMF-R) is a cell surface receptor that is also localized to a smooth subdomain of the endoplasmic reticulum, the AMF-R tubule. By postembedding immunoelectron microscopy, AMF-R concentrates within smooth plasmalemmal vesicles or caveolae in both NIH-3T3 fibroblasts and HeLa cells. By confocal microscopy, cell surface AMF-R labeled by the addition of anti-AMF-R antibody to viable cells at 4\u00b0C exhibits partial colocalization with caveolin, confirming the localization of cell surface AMF-R to caveolae. Labeling of cell surface AMF-R by either anti-AMF-R antibody or biotinylated AMF (bAMF) exhibits extensive colocalization and after a pulse of 1\u20132 h at 37\u00b0C, bAMF accumulates in densely labeled perinuclear structures as well as fainter tubular structures that colocalize with AMF-R tubules. After a subsequent 2- to 4-h chase, bAMF is localized predominantly to AMF-R tubules. Cytoplasmic acidification, blocking clathrin-mediated endocytosis, results in the essentially exclusive distribution of internalized bAMF to AMF-R tubules. By confocal microscopy, the tubular structures labeled by internalized bAMF show complete colocalization with AMF-R tubules. bAMF internalized in the presence of a 10-fold excess of unlabeled AMF labels perinuclear punctate structures, which are therefore the product of fluid phase endocytosis, but does not label AMF-R tubules, demonstrating that bAMF targeting to AMF-R tubules occurs via a receptor-mediated pathway. By electron microscopy, bAMF internalized for 10 min is located to cell surface caveolae and after 30 min is present within smooth and rough endoplasmic reticulum tubules. AMF-R is therefore internalized via a receptor-mediated clathrin-independent pathway to smooth ER. The steady state localization of AMF-R to caveolae implicates these cell surface invaginations in AMF-R endocytosis.<\/jats:p>","DOI":"10.1091\/mbc.9.7.1773","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T23:13:15Z","timestamp":1372806795000},"page":"1773-1786","source":"Crossref","is-referenced-by-count":98,"title":["Localization of Autocrine Motility Factor Receptor to Caveolae and Clathrin-independent Internalization of Its Ligand to Smooth Endoplasmic Reticulum"],"prefix":"10.1091","volume":"9","author":[{"given":"Naciba","family":"Benlimame","sequence":"first","affiliation":[{"name":"D\u00e9partement de Pathologie et Biologie Cellulaire, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada H3C 3J7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phuong U.","family":"Le","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pathologie et Biologie Cellulaire, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada H3C 3J7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan R.","family":"Nabi","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pathologie et Biologie Cellulaire, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada H3C 3J7"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1076","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1083\/jcb.120.6.1449","volume":"120","author":"Altankov G.","year":"1993","journal-title":"J. Cell Biol."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.7.11.1825"},{"key":"B3","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1126\/science.1310359","volume":"253","author":"Anderson R.G.W.","year":"1992","journal-title":"Science"},{"key":"B4","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1242\/jcs.109.7.1857","volume":"109","author":"Bendayan M.","year":"1996","journal-title":"J. Cell Sci."},{"key":"B5","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1083\/jcb.129.2.459","volume":"129","author":"Benlimame N.","year":"1995","journal-title":"J. Cell Biol."},{"key":"B6","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1073\/pnas.80.5.1367","volume":"80","author":"Bergmann J.E.","year":"1983","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B7","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1126\/science.6719108","volume":"226","author":"Bretscher M.S.","year":"1984","journal-title":"Science"},{"key":"B8","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1002\/j.1460-2075.1989.tb03514.x","volume":"8","author":"Bretscher M.S.","year":"1989","journal-title":"EMBO J."},{"key":"B9","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/S0092-8674(00)81380-X","volume":"87","author":"Bretscher M.S.","year":"1996","journal-title":"Cell"},{"key":"B10","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/0092-8674(92)90189-J","volume":"68","author":"Brown D.A.","year":"1992","journal-title":"Cell"},{"key":"B11","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/1047-8477(91)90033-S","volume":"107","author":"Bundgaard M.","year":"1991","journal-title":"J. Struct. Biol."},{"key":"B12","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1083\/jcb.126.1.127","volume":"126","author":"Chang W.-J.","year":"1994","journal-title":"J. Cell Biol."},{"key":"B13","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0955-0674(95)80045-X","volume":"7","author":"Cole N.B.","year":"1995","journal-title":"Curr. Opin. Cell Biol."},{"key":"B14","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1146\/annurev.cb.09.110193.002211","volume":"9","author":"Condeelis J.","year":"1993","journal-title":"Annu. Rev. Cell Biol."},{"key":"B15","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1083\/jcb.131.6.1421","volume":"131","author":"Conrad P.A.","year":"1995","journal-title":"J. Cell Biol."},{"key":"B16","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1083\/jcb.127.3.725","volume":"127","author":"Cupers P.","year":"1994","journal-title":"J. Cell Biol."},{"key":"B17","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0092-8674(90)90240-F","volume":"62","author":"Dotti C.G.","year":"1990","journal-title":"Cell"},{"key":"B18","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0092-8674(00)81278-7","volume":"84","author":"Drubin D.G.","year":"1996","journal-title":"Cell"},{"key":"B19","doi-asserted-by":"crossref","first-page":"8655","DOI":"10.1073\/pnas.92.19.8655","volume":"92","author":"Fra A.M.","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B20","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1083\/jcb.119.6.1507","volume":"119","author":"Fujimoto T.","year":"1992","journal-title":"J. Cell Biol."},{"key":"B21","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1083\/jcb.125.1.129","volume":"125","author":"Garrigues J.","year":"1994","journal-title":"J. Cell Biol."},{"key":"B22","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1083\/jcb.102.4.1304","volume":"102","author":"Ghitescu L.","year":"1986","journal-title":"J. Cell Biol."},{"key":"B23","doi-asserted-by":"crossref","first-page":"20163","DOI":"10.1016\/S0021-9258(19)47038-5","volume":"264","author":"Glenney J.R.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"B24","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1097\/00043764-199009000-00017","volume":"32","author":"Guirguis R.","year":"1990","journal-title":"J. Occup. Med."},{"key":"B25","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1083\/jcb.123.1.89","volume":"123","author":"Hansen S.H.","year":"1993","journal-title":"J. Cell Biol."},{"key":"B26","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1083\/jcb.108.2.401","volume":"108","author":"Heuser J.","year":"1989","journal-title":"J. Cell Biol."},{"key":"B27","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1038\/bjc.1996.667","volume":"74","author":"Hirono Y.","year":"1996","journal-title":"Br. J. Cancer"},{"key":"B28","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1083\/jcb.125.6.1265","volume":"125","author":"Hopkins C.R.","year":"1994","journal-title":"J. Cell Biol."},{"key":"B29","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/0167-4889(94)90046-9","volume":"1222","author":"Kanbe K.","year":"1994","journal-title":"Biochim. Biophys. Acta"},{"key":"B30","doi-asserted-by":"crossref","first-page":"2721","DOI":"10.1083\/jcb.109.6.2721","volume":"109","author":"Kartenbeck J.","year":"1989","journal-title":"J. Cell Biol."},{"key":"B31","doi-asserted-by":"crossref","first-page":"3001","DOI":"10.1242\/jcs.110.24.3001","volume":"110","author":"Keller P.","year":"1997","journal-title":"J. Cell Sci."},{"key":"B32","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/0092-8674(90)90206-T","volume":"61","author":"Kelly R.B.","year":"1990","journal-title":"Cell"},{"key":"B33","first-page":"19","volume":"73","author":"Kiss A.L.","year":"1997","journal-title":"Eur. J. Cell Biol."},{"key":"B34","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1073\/pnas.92.5.1381","volume":"92","author":"Koleske A.J.","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B35","doi-asserted-by":"crossref","first-page":"28647","DOI":"10.1074\/jbc.271.45.28647","volume":"271","author":"Li S.","year":"1996","journal-title":"J. Biol. Chem."},{"key":"B36","doi-asserted-by":"crossref","first-page":"3302","DOI":"10.1073\/pnas.83.10.3302","volume":"83","author":"Liotta L.A.","year":"1986","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B37","doi-asserted-by":"crossref","first-page":"13666","DOI":"10.1073\/pnas.94.25.13666","volume":"94","author":"Liu P.","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B38","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/296651a0","volume":"296","author":"Montesano R.","year":"1982","journal-title":"Nature"},{"key":"B39","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1177\/002215549704501004","volume":"45","author":"Nabi I.R.","year":"1997","journal-title":"J. Histochem. Cytochem."},{"key":"B40","first-page":"409","volume":"50","author":"Nabi I.R.","year":"1990","journal-title":"Cancer Res."},{"key":"B41","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/BF00047599","volume":"11","author":"Nabi I.R.","year":"1992","journal-title":"Cancer Metastasis Rev."},{"key":"B42","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1002\/1097-0142(19941001)74:7<1855::AID-CNCR2820740705>3.0.CO;2-1","volume":"74","author":"Nakamori S.","year":"1994","journal-title":"Cancer"},{"key":"B43","doi-asserted-by":"crossref","first-page":"8342","DOI":"10.1016\/S0021-9258(18)42449-0","volume":"267","author":"Okamoto T.","year":"1992","journal-title":"J. Biol. Chem."},{"key":"B44","first-page":"3120","volume":"54","author":"Otto T.","year":"1994","journal-title":"Cancer Res."},{"key":"B45","first-page":"11","volume":"463","author":"Palade G.","year":"1979","journal-title":"Acta Physiol. Scand."},{"key":"B46","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1083\/jcb.127.5.1199","volume":"127","author":"Parton R.G.","year":"1994","journal-title":"J. Cell Biol."},{"key":"B47","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1083\/jcb.135.1.153","volume":"135","author":"Per\u00e4nen J.","year":"1996","journal-title":"J. Cell Biol."},{"key":"B48","first-page":"340","volume":"50","author":"Raposo G.","year":"1989","journal-title":"Eur. J. Cell Biol."},{"key":"B49","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1083\/jcb.106.3.747","volume":"106","author":"Rinnerthaler G.","year":"1988","journal-title":"J. Cell Biol."},{"key":"B50","doi-asserted-by":"crossref","first-page":"3507","DOI":"10.1128\/MCB.15.7.3507","volume":"15","author":"Robbins S.M.","year":"1995","journal-title":"Mol. Cell Biol."},{"key":"B51","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev.cb.08.110192.002143","volume":"8","author":"Rodriguez-Boulan E.","year":"1992","journal-title":"Annu. Rev. Cell Biol."},{"key":"B52","doi-asserted-by":"crossref","first-page":"5071","DOI":"10.1073\/pnas.75.10.5071","volume":"75","author":"Rodriguez-Boulan E.","year":"1978","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B53","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1083\/jcb.128.6.1029","volume":"128","author":"Roettger B.F.","year":"1995","journal-title":"J. Cell Biol."},{"key":"B54","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1083\/jcb.99.3.1101","volume":"99","author":"Rogalski A.A.","year":"1984","journal-title":"J. Cell Biol."},{"key":"B55","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/0092-8674(92)90143-Z","volume":"68","author":"Rothberg K.G.","year":"1992","journal-title":"Cell"},{"key":"B56","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1083\/jcb.105.2.679","volume":"105","author":"Sandvig K.","year":"1987","journal-title":"J. Cell Biol."},{"key":"B57","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1083\/jcb.122.4.789","volume":"122","author":"Sargiacomo M.","year":"1993","journal-title":"J. Cell Biol."},{"key":"B58","doi-asserted-by":"crossref","first-page":"14399","DOI":"10.1074\/jbc.270.24.14399","volume":"270","author":"Schnitzer J.E.","year":"1995","journal-title":"J. Biol. Chem."},{"key":"B59","first-page":"1217","volume":"127","author":"Schnitzer J.E.","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B60","doi-asserted-by":"crossref","first-page":"7444","DOI":"10.1016\/S0021-9258(18)42537-9","volume":"267","author":"Sharp A.H.","year":"1992","journal-title":"J. Biol. Chem."},{"key":"B61","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1083\/jcb.126.2.353","volume":"126","author":"Shenoy-Scaria A.M.","year":"1994","journal-title":"J. Cell Biol."},{"key":"B62","first-page":"1649","volume":"148","author":"Silletti S.","year":"1996","journal-title":"Am. J. Pathol."},{"key":"B63","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1006\/bbrc.1993.1840","volume":"194","author":"Silletti S.","year":"1993","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B64","first-page":"137","volume":"213","author":"Silletti S.","year":"1996","journal-title":"Curr. Topics Microbiol. Immunol."},{"key":"B65","first-page":"5752","volume":"54","author":"Silletti S.","year":"1994","journal-title":"Cancer Res."},{"key":"B66","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1006\/bbrc.1996.0192","volume":"219","author":"Simard D.","year":"1996","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B67","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1146\/annurev.cb.02.110186.002005","volume":"2","author":"Singer S.J.","year":"1986","journal-title":"Annu. Rev. Cell Biol."},{"key":"B68","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1083\/jcb.124.3.307","volume":"124","author":"Smart E.J.","year":"1993","journal-title":"J. Cell Biol."},{"key":"B69","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1083\/jcb.127.5.1185","volume":"127","author":"Smart E.J.","year":"1994","journal-title":"J. Cell Biol."},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.4.595"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.1.47"},{"key":"B72","first-page":"81","volume":"1072","author":"Stoker M.","year":"1991","journal-title":"Biochim. Biophys. Acta"},{"key":"B73","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1126\/science.8493552","volume":"260","author":"Stossel T.P.","year":"1993","journal-title":"Science"},{"key":"B74","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/0006-291X(87)90730-3","volume":"146","author":"Stracke M.L.","year":"1987","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"B75","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/0092-8674(93)90638-7","volume":"73","author":"Takizawa P.A.","year":"1993","journal-title":"Cell"},{"key":"B76","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1083\/jcb.128.1.139","volume":"128","author":"Tanaka E.","year":"1995","journal-title":"J. Cell Biol."},{"key":"B77","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1002\/ijc.2910550621","volume":"55","author":"Timar J.","year":"1993","journal-title":"Int. J. Cancer"},{"key":"B78","doi-asserted-by":"crossref","first-page":"7957","DOI":"10.1073\/pnas.84.22.7957","volume":"84","author":"Tran D.","year":"1987","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"B79","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0962-8924(93)90045-3","volume":"3","author":"van Deurs B.","year":"1993","journal-title":"Trends Cell Biol."},{"key":"B80","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.1242\/jcs.110.24.3043","volume":"110","author":"Wang H.-J.","year":"1997","journal-title":"J. Cell Sci."},{"key":"B81","doi-asserted-by":"crossref","first-page":"13442","DOI":"10.1016\/S0021-9258(18)98859-9","volume":"266","author":"Watanabe H.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"B82","first-page":"2960","volume":"56","author":"Watanabe H.","year":"1996","journal-title":"Cancer Res."},{"key":"B83","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1083\/jcb.133.2.247","volume":"133","author":"Yoshimoro T.","year":"1996","journal-title":"J. Cell Biol."}],"container-title":["Molecular Biology of the Cell"],"original-title":[],"language":"en","deposited":{"date-parts":[[2022,2,27]],"date-time":"2022-02-27T02:36:43Z","timestamp":1645929403000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.molbiolcell.org\/doi\/10.1091\/mbc.9.7.1773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,7]]},"references-count":83,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1998,7]]}},"alternative-id":["10.1091\/mbc.9.7.1773"],"URL":"https:\/\/doi.org\/10.1091\/mbc.9.7.1773","relation":{},"ISSN":["1059-1524","1939-4586"],"issn-type":[{"value":"1059-1524","type":"print"},{"value":"1939-4586","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,7]]}}}