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An arginine substitution of K418 abolishes SUMO-1 conjugation and although it does not interfere with ADAR1 proper localization, it stimulates the ability of the enzyme to edit RNA both in vivo and in vitro. Moreover, modification of wild-type recombinant ADAR1 by SUMO-1 reduces the editing activity of the enzyme in vitro. Taken together these data suggest a novel role for sumoylation in regulating RNA-editing activity.<\/jats:p>","DOI":"10.1091\/mbc.e05-06-0536","type":"journal-article","created":{"date-parts":[[2005,8,25]],"date-time":"2005-08-25T00:24:24Z","timestamp":1124929464000},"page":"5115-5126","source":"Crossref","is-referenced-by-count":109,"title":["SUMO-1 Modification Alters ADAR1 Editing Activity"],"prefix":"10.1091","volume":"16","author":[{"given":"Joana M.P.","family":"Desterro","sequence":"first","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]},{"given":"Liam P.","family":"Keegan","sequence":"additional","affiliation":[{"name":"MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland, United Kingdom"}]},{"given":"Ellis","family":"Jaffray","sequence":"additional","affiliation":[{"name":"Biomolecular Sciences Building, University of St. Andrews, St. Andrews, Fife KY19 9ST, Scotland, United Kingdom"}]},{"given":"Ron T.","family":"Hay","sequence":"additional","affiliation":[{"name":"Biomolecular Sciences Building, University of St. Andrews, St. Andrews, Fife KY19 9ST, Scotland, United Kingdom"}]},{"given":"Mary A.","family":"O'Connell","sequence":"additional","affiliation":[{"name":"MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, Scotland, United Kingdom"}]},{"given":"Maria","family":"Carmo-Fonseca","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal"}]}],"member":"1076","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Andersen, J. 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