{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:35:42Z","timestamp":1767854142985,"version":"3.49.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:00:00Z","timestamp":1605571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Warshel Institute for Computational Biology"},{"name":"School of Life and Health Sciences"},{"DOI":"10.13039\/501100004853","name":"Chinese University of Hong Kong","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004853","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG3F1721"],"award-info":[{"award-number":["CMRPG3F1721"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG3F1722"],"award-info":[{"award-number":["CMRPG3F1722"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPD3I0011"],"award-info":[{"award-number":["CMRPD3I0011"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>A mass spectrometry-based assessment of methicillin resistance in Staphylococcus aureus would have huge potential in addressing fast and effective prediction of antibiotic resistance. Since delays in the traditional antibiotic susceptibility testing, methicillin-resistant S. aureus remains a serious threat to human health.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Here, linking a 7\u00a0years of longitudinal study from two cohorts in the Taiwan area of over 20\u00a0000 individually resolved methicillin susceptibility testing results, we identify associations of methicillin resistance with the demographics and mass spectrometry data. When combined together, these connections allow for machine-learning-based predictions of methicillin resistance, with an area under the receiver operating characteristic curve of &amp;gt;0.85 in both the discovery [95% confidence interval (CI) 0.88\u20130.90] and replication (95% CI 0.84\u20130.86) populations.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our predictive model facilitates early detection for methicillin resistance of patients with S. aureus infection. The large-scale antibiotic resistance study has unbiasedly highlighted putative candidates that could improve trials of treatment efficiency and inform on prescriptions.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bib\/bbaa293","type":"journal-article","created":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T19:09:39Z","timestamp":1602184179000},"source":"Crossref","is-referenced-by-count":15,"title":["Large-scale mass spectrometry data combined with demographics analysis rapidly predicts methicillin resistance in <i>Staphylococcus aureus<\/i>"],"prefix":"10.1093","volume":"22","author":[{"given":"Zhuo","family":"Wang","sequence":"first","affiliation":[{"name":"Warshel Institute for Computational Biology, The Chinese University of Hong Kong, Shenzhen, Shenzhen, China"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5581-6793","authenticated-orcid":false,"given":"Hsin-Yao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City, Taiwan"}]},{"given":"Chia-Ru","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Information Engineering, National Central University, Taoyuan City, Taiwan"}]},{"given":"Jorng-Tzong","family":"Horng","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Information Engineering, National Central University, Taiwan"}]},{"given":"Jang-Jih","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City, Taiwan"}]},{"given":"Tzong-Yi","family":"Lee","sequence":"additional","affiliation":[{"name":"Warshel Institute for Computational Biology, School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, Shenzhen, China"}]}],"member":"286","published-online":{"date-parts":[[2020,11,17]]},"reference":[{"key":"2021072117030967600_ref1","first-page":"1","volume-title":"Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols","author":"Palavecino","year":"2014"},{"key":"2021072117030967600_ref2","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.3389\/fmicb.2018.01097","article-title":"Recent advances and ongoing challenges in the diagnosis of microbial infections by MALDI-TOF mass spectrometry","volume":"9","author":"Florio","year":"2018","journal-title":"Front Microbiol"},{"key":"2021072117030967600_ref3","doi-asserted-by":"crossref","first-page":"475","DOI":"10.3343\/alm.2017.37.6.475","article-title":"Matrix-assisted laser desorption ionization time-of-flight mass spectrometry in clinical microbiology: what are the current issues?","volume":"37","author":"Van Belkum","year":"2017","journal-title":"Ann Lab 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aureus","volume":"333","author":"Maple","year":"1989","journal-title":"Lancet"},{"key":"2021072117030967600_ref7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nrdp.2018.33","article-title":"Methicillin-resistant Staphylococcus aureus","volume":"4","author":"Lee","year":"2018","journal-title":"Nat Rev Dis Primers"},{"key":"2021072117030967600_ref8","doi-asserted-by":"crossref","first-page":"D285","DOI":"10.1093\/nar\/gky1030","article-title":"dbAMP: an integrated resource for exploring antimicrobial peptides with functional activities and physicochemical properties on transcriptome and proteome data","volume":"47","author":"Jhong","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2021072117030967600_ref9","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/s41579-018-0147-4","article-title":"Methicillin-resistant Staphylococcus aureus: an overview of basic and clinical research","volume":"17","author":"Turner","year":"2019","journal-title":"Nat Rev Microbiol"},{"key":"2021072117030967600_ref10","doi-asserted-by":"crossref","first-page":"3785","DOI":"10.1128\/JCM.39.10.3785-3788.2001","article-title":"Performance of eight methods, including two new rapid methods, for detection of oxacillin resistance in a challenge set of Staphylococcus aureus organisms","volume":"39","author":"Swenson","year":"2001","journal-title":"J Clin Microbiol"},{"key":"2021072117030967600_ref11","doi-asserted-by":"crossref","first-page":"3946","DOI":"10.1128\/JCM.39.11.3946-3951.2001","article-title":"Methicillin-resistant Staphylococcus aureus: comparison of susceptibility testing methods and analysis of mecA-positive susceptible strains","volume":"39","author":"Sakoulas","year":"2001","journal-title":"J Clin Microbiol"},{"key":"2021072117030967600_ref12","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1128\/CMR.00097-16","article-title":"A decade of development of chromogenic culture media for clinical microbiology in an era of molecular 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