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Selected mutations in SARS-CoV-2\u00a0S-protein are reported to strengthen its binding affinity to mammalian ACE2. The N501T mutation in SARS-CoV-2-CTD furnishes better support to hotspot 353 in comparison with SARS-CoV and shows higher affinity for receptor binding. Recombination analysis exhibited higher recombination events in SARS-CoV-2 strains, irrespective of their geographical origin or hosts. Investigation further supports a common origin among SARS-CoV-2 and its predecessors, SARS-CoV and bat-SARS-like-CoV. The recombination events suggest a constant exchange of genetic material among the co-infecting viruses in possible reservoirs and human hosts before SARS-CoV-2 emerged. Furthermore, a comprehensive analysis of codon usage bias (CUB) in SARS-CoV-2 revealed significant CUB among the S-genes of different beta-coronaviruses governed majorly by natural selection and mutation pressure. Various indices of codon usage of S-genes helped in quantifying its adaptability in other animal hosts. These findings might help in identifying potential experimental animal models for investigating pathogenicity for drugs and vaccine development experiments.<\/jats:p>","DOI":"10.1093\/bib\/bbaa383","type":"journal-article","created":{"date-parts":[[2020,12,12]],"date-time":"2020-12-12T12:39:12Z","timestamp":1607776752000},"page":"1006-1022","source":"Crossref","is-referenced-by-count":22,"title":["Evolutionary and codon usage preference insights into spike glycoprotein of SARS-CoV-2"],"prefix":"10.1093","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-4854","authenticated-orcid":false,"given":"Yashpal Singh","family":"Malik","sequence":"first","affiliation":[{"name":"College of Animal Biotechnology, GADVASU, Ludhiana"}]},{"given":"Mohd Ikram","family":"Ansari","sequence":"additional","affiliation":[{"name":"Aligarh Muslim University India"}]},{"given":"Jobin Jose","family":"Kattoor","sequence":"additional","affiliation":[{"name":"ICAR-Indian Veterinary Research Institute, India"}]},{"given":"Rahul","family":"Kaushik","sequence":"additional","affiliation":[{"name":"Laboratory for Structural Bioinformatics, RIKEN Center for Biosystems Dynamics Research, Japan"}]},{"given":"Shubhankar","family":"Sircar","sequence":"additional","affiliation":[{"name":"Integral University, India"}]},{"given":"Anbazhagan","family":"Subbaiyan","sequence":"additional","affiliation":[{"name":"ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India"}]},{"given":"Ruchi","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Department of Vet erinary Microbiology, DUVASU, Mathura, India"}]},{"given":"Kuldeep","family":"Dhama","sequence":"additional","affiliation":[{"name":"Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India"}]},{"given":"Souvik","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Health Center for Zoonoses and Tropical Veterinary Medicine, Basseterre, St. Kitts, West 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