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However, the lack of a comprehensive compound library or customizable bioinformatics tool is currently a challenge in GC\u2009\u00d7\u2009GC\u2013TOFMS data analysis. We present an open-source deep learning (DL) software called contour regions of interest (ROI) identification, simulation and untargeted metabolomics profiler (CRISP). CRISP integrates multiple customizable deep neural network architectures for assisting the semi-automated identification of ROIs, contour synthesis, resolution enhancement and classification of GC\u2009\u00d7\u2009GC\u2013TOFMS-based contour images. The approach includes the novel aggregate feature representative contour (AFRC) construction and stacked ROIs. This generates an unbiased contour image dataset that enhances the contrasting characteristics between different test groups and can be suitable for small sample sizes. The utility of the generative models and the accuracy and efficacy of the platform were demonstrated using a dataset of GC\u2009\u00d7\u2009GC\u2013TOFMS contour images from patients with late-stage diabetic nephropathy and healthy control groups. CRISP successfully constructed AFRC images and identified over five ROIs to create a deepstacked dataset. The high fidelity, 512\u2009\u00d7\u2009512-pixels generative model was trained as a generator with a Fr\u00e9chet inception distance of &amp;lt;47.00. The trained classifier achieved an AUROC of &amp;gt;0.96 and a classification accuracy of &amp;gt;95.00% for datasets with and without column bleed. Overall, CRISP demonstrates good potential as a DL-based approach for the rapid analysis of 4-D GC\u2009\u00d7\u2009GC\u2013TOFMS untargeted metabolite profiles by directly implementing contour images. CRISP is available at https:\/\/github.com\/vivekmathema\/GCxGC-CRISP.<\/jats:p>","DOI":"10.1093\/bib\/bbab550","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T12:07:22Z","timestamp":1638274042000},"source":"Crossref","is-referenced-by-count":11,"title":["CRISP: a deep learning architecture for GC\u2009\u00d7\u2009GC\u2013TOFMS contour ROI identification, simulation and analysis in imaging metabolomics"],"prefix":"10.1093","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3916-9949","authenticated-orcid":false,"given":"Vivek Bhakta","family":"Mathema","sequence":"first","affiliation":[{"name":"Metabolomics and Systems Biology, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Siriraj Metabolomics and Phenomics Center, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}]},{"given":"Kassaporn","family":"Duangkumpha","sequence":"additional","affiliation":[{"name":"Metabolomics and Systems Biology, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Siriraj Metabolomics and Phenomics Center, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}]},{"given":"Kwanjeera","family":"Wanichthanarak","sequence":"additional","affiliation":[{"name":"Metabolomics and Systems Biology, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Siriraj Metabolomics and Phenomics Center, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}]},{"given":"Narumol","family":"Jariyasopit","sequence":"additional","affiliation":[{"name":"Metabolomics and Systems Biology, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Siriraj Metabolomics and Phenomics Center, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}]},{"given":"Esha","family":"Dhakal","sequence":"additional","affiliation":[{"name":"Metabolomics and Systems Biology, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Siriraj Metabolomics and Phenomics Center, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}]},{"given":"Nuankanya","family":"Sathirapongsasuti","sequence":"additional","affiliation":[{"name":"Section of Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand"},{"name":"Research Network of NANOTEC \u2013 MU Ramathibodi on Nanomedicine, Bangkok, Thailand"}]},{"given":"Chagriya","family":"Kitiyakara","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Rama VI Rd., Ratchathewi, Bangkok 10400, Thailand"}]},{"given":"Yongyut","family":"Sirivatanauksorn","sequence":"additional","affiliation":[{"name":"Siriraj Metabolomics and Phenomics Center, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9461-8597","authenticated-orcid":false,"given":"Sakda","family":"Khoomrung","sequence":"additional","affiliation":[{"name":"Metabolomics and Systems Biology, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Siriraj Metabolomics and Phenomics Center, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Center of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Mahidol University, Bangkok, Thailand"}]}],"member":"286","published-online":{"date-parts":[[2022,1,11]]},"reference":[{"key":"2022031506245172800_ref1","first-page":"85","article-title":"Plasma esterified and non-esterified fatty acids metabolic profiling using gas chromatography\u2013mass spectrometry and its application in the study of diabetic mellitus and diabetic nephropathy","volume-title":"Anal. 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