{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:08:34Z","timestamp":1773011314837,"version":"3.50.1"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771469"],"award-info":[{"award-number":["31771469"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["2017YFC0908500"],"award-info":[{"award-number":["2017YFC0908500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Exosomes cargo tumour-characterized biomolecules secreted from cancer cells and play a pivotal role in tumorigenesis and cancer progression, thus providing their potential for non-invasive cancer monitoring. Since cancer cell-derived exosomes are often mixed with those from healthy cells in liquid biopsy of tumour patients, accurately measuring the purity of tumour cell-derived exosomes is not only critical for the early detection but also essential for unbiased identification of diagnosis biomarkers. Here, we propose \u2018ExosomePurity\u2019, a tumour purity deconvolution model to estimate tumour purity in serum exosomes of cancer patients based on microribonucleic acid (miRNA)-Seq data. We first identify the differently expressed miRNAs as signature to distinguish cancer cell- from healthy cell-derived exosomes. Then, the deconvolution model was developed to estimate the proportions of cancer exosomes and normal exosomes in serum. The purity predicted by the model shows high correlation with actual purity in simulated data and actual data. Moreover, the model is robust under the different levels of noise background. The tumour purity was also used to correct differential expressed gene analysis. ExosomePurity empowers the research community to study non-invasive early diagnosis and to track cancer progression in cancers more efficiently. It is implemented in R and is freely available from GitHub (https:\/\/github.com\/WangHYLab\/ExosomePurity).<\/jats:p>","DOI":"10.1093\/bib\/bbad119","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T14:00:48Z","timestamp":1679666448000},"source":"Crossref","is-referenced-by-count":4,"title":["ExosomePurity: tumour purity deconvolution in serum exosomes based on miRNA signatures"],"prefix":"10.1093","volume":"24","author":[{"given":"Tao","family":"Wu","sequence":"first","affiliation":[{"name":"School of Life Sciences and Technology, Tongji University , Shanghai 200092 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Dai","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Tongji University , Shanghai 200092 , China"},{"name":"Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital\uff0cShanghai Jiao Tong University School of Medicine , Shanghai , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Tongji University , Shanghai 200092 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Tongji University , Shanghai 200092 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuting","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Tongji University , Shanghai 200092 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Tongji University , Shanghai 200092 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Tongji University , Shanghai 200092 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqi","family":"Zheng","sequence":"additional","affiliation":[{"name":"Center for Single-Cell Omics , School of Public Health, , Shanghai , China"},{"name":"Shanghai Jiao Tong University School of Medicine , School of Public Health, , Shanghai , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyun","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Technology, Tongji University , Shanghai 200092 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"2023052022113706900_ref1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/nature21349","article-title":"Elements of cancer immunity and the cancer-immune set point","volume":"541","author":"Chen","year":"2017","journal-title":"Nature"},{"key":"2023052022113706900_ref2","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1038\/nature25183","article-title":"The biology and management of non-small cell lung cancer","volume":"553","author":"Herbst","year":"2018","journal-title":"Nature"},{"key":"2023052022113706900_ref3","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nature13385","article-title":"Comprehensive molecular profiling of lung 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