{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:10:38Z","timestamp":1770887438968,"version":"3.50.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81830053"],"award-info":[{"award-number":["81830053"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["61972084"],"award-info":[{"award-number":["61972084"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["61871121"],"award-info":[{"award-number":["61871121"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Research and Development Program in Jiangsu Province","award":["BE2022828"],"award-info":[{"award-number":["BE2022828"]}]},{"name":"Jiangsu Funding Program for Excellent Postdoctoral Talent","award":["2022ZB699"],"award-info":[{"award-number":["2022ZB699"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,19]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Undoubtedly, single-cell RNA sequencing (scRNA-seq) has changed the research landscape by providing insights into heterogeneous, complex and rare cell populations. Given that more such data sets will become available in the near future, their accurate assessment with compatible and robust models for cell type annotation is a prerequisite. Considering this, herein, we developed scAnno (scRNA-seq data annotation), an automated annotation tool for scRNA-seq data sets primarily based on the single-cell cluster levels, using a joint deconvolution strategy and logistic regression. We explicitly constructed a reference profile for human (30 cell types and 50 human tissues) and a reference profile for mouse (26 cell types and 50 mouse tissues) to support this novel methodology (scAnno). scAnno offers a possibility to obtain genes with high expression and specificity in a given cell type as cell type-specific genes (marker genes) by combining co-expression genes with seed genes as a core. Of importance, scAnno can accurately identify cell type-specific genes based on cell type reference expression profiles without any prior information. Particularly, in the peripheral blood mononuclear cell data set, the marker genes identified by scAnno showed cell type-specific expression, and the majority of marker genes matched exactly with those included in the CellMarker database. Besides validating the flexibility and interpretability of scAnno in identifying marker genes, we also proved its superiority in cell type annotation over other cell type annotation tools (SingleR, scPred, CHETAH and scmap-cluster) through internal validation of data sets (average annotation accuracy: 99.05%) and cross-platform data sets (average annotation accuracy: 95.56%). Taken together, we established the first novel methodology that utilizes a deconvolution strategy for automated cell typing and is capable of being a significant application in broader scRNA-seq analysis. scAnno is available at https:\/\/github.com\/liuhong-jia\/scAnno.<\/jats:p>","DOI":"10.1093\/bib\/bbad179","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T07:23:26Z","timestamp":1684135406000},"source":"Crossref","is-referenced-by-count":13,"title":["scAnno: a deconvolution strategy-based automatic cell type annotation tool for single-cell RNA-sequencing data sets"],"prefix":"10.1093","volume":"24","author":[{"given":"Hongjia","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Digital Medical Engineering , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"},{"name":"Southeast University , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huamei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Surgery , Nanjing Drum Tower Hospital, , Nanjing, 210008 , PR China"},{"name":"the Affiliated Hospital of Nanjing University Medical School , Nanjing Drum Tower Hospital, , Nanjing, 210008 , PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, University Hospital Bonn , Bonn , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Southeast University Hospital , Nanjing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duo","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Digital Medical Engineering , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"},{"name":"Southeast University , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Gu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Digital Medical Engineering , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"},{"name":"Southeast University , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Digital Medical Engineering , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"},{"name":"Southeast University , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Digital Medical Engineering , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"},{"name":"Southeast University , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongde","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Digital Medical Engineering , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"},{"name":"Southeast University , School of Biological Science & Medical Engineering, , Nanjing, 210096 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,5,14]]},"reference":[{"key":"2023052022430665400_ref1","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1016\/j.csbj.2021.01.015","article-title":"Automated methods for cell type annotation on scRNA-seq data","volume":"19","author":"Pasquini","year":"2021","journal-title":"Comput Struct Biotechnol J"},{"key":"2023052022430665400_ref2","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1038\/s41467-020-15523-2","article-title":"SciBet as a portable and fast single cell type identifier","volume":"11","author":"Li","year":"2020","journal-title":"Nat Commun"},{"key":"2023052022430665400_ref3","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/s41576-018-0088-9","article-title":"Challenges in unsupervised clustering of single-cell RNA-seq data","volume":"20","author":"Kiselev","year":"2019","journal-title":"Nat Rev 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