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However, the high costs associated with this technique have rendered it impractical for studying large patient cohorts. We introduce ENIGMA (Deconvolution based on Regularized Matrix Completion), a method that addresses this limitation through accurately deconvoluting bulk tissue RNA-seq data into a readout with cell-type resolution by leveraging information from scRNA-seq data. By employing a matrix completion strategy, ENIGMA minimizes the distance between the mixture transcriptome obtained with bulk sequencing and a weighted combination of cell-type-specific expression. This allows the quantification of cell-type proportions and reconstruction of cell-type-specific transcriptomes. To validate its performance, ENIGMA was tested on both simulated and real datasets, including disease-related tissues, demonstrating its ability in uncovering novel biological insights.<\/jats:p>","DOI":"10.1093\/bib\/bbad273","type":"journal-article","created":{"date-parts":[[2023,8,2]],"date-time":"2023-08-02T08:58:27Z","timestamp":1690966707000},"source":"Crossref","is-referenced-by-count":8,"title":["Approximate estimation of cell-type resolution transcriptome in bulk tissue through matrix completion"],"prefix":"10.1093","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8482-539X","authenticated-orcid":false,"given":"Weixu","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Genetic Engineering, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Collaborative Innovation Center of Genetics and Development, Human Phenome Institute, Center for Evolutionary Biology, Shanghai Engineering Research Center of Industrial Microorganisms, School of Life Sciences, Fudan University , Shanghai 200438 , P.R. China"}]},{"given":"Xiaolan","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Collaborative Innovation Center of Genetics and Development, Human Phenome Institute, Center for Evolutionary Biology, Shanghai Engineering Research Center of Industrial Microorganisms, School of Life Sciences, Fudan University , Shanghai 200438 , P.R. China"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Collaborative Innovation Center of Genetics and Development, Human Phenome Institute, Center for Evolutionary Biology, Shanghai Engineering Research Center of Industrial Microorganisms, School of Life Sciences, Fudan University , Shanghai 200438 , P.R. China"}]},{"given":"Jun","family":"Yao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Collaborative Innovation Center of Genetics and Development, Human Phenome Institute, Center for Evolutionary Biology, Shanghai Engineering Research Center of Industrial Microorganisms, School of Life Sciences, Fudan University , Shanghai 200438 , P.R. China"}]},{"given":"Haimei","family":"Wen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering, National Clinical Research Center for Aging and Medicine, Huashan Hospital, Collaborative Innovation Center of Genetics and Development, Human Phenome Institute, Center for Evolutionary Biology, Shanghai Engineering Research Center of Industrial Microorganisms, School of Life Sciences, Fudan University , Shanghai 200438 , P.R. China"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology, Human Phenome Institute, School of Life Sciences, Fudan University , Shanghai 200438 , P.R. China"}]},{"given":"Mingwan","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Gene Engineering of the Ministry of Education and State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University , Guangzhou 510006 , P.R. China"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Cell Proliferation and Differentiation , School of Life Sciences, , Beijing 100871 , P.R. China"},{"name":"Peking University , School of Life Sciences, , Beijing 100871 , P.R. China"}]},{"given":"Wei","family":"Tao","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Cell Proliferation and Differentiation , School of Life Sciences, , Beijing 100871 , P.R. China"},{"name":"Peking University , School of Life Sciences, , Beijing 100871 , P.R. China"}]},{"given":"Jiahua","family":"Zou","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Bioengineering Medicine , National Engineering Research Center of Genetic Medicine, Institute of Biomedicine, , Guangzhou 510632 , P.R. China"},{"name":"College of Life Science and Technology, Jinan University , National Engineering Research Center of Genetic Medicine, Institute of Biomedicine, , Guangzhou 510632 , P.R. 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