{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T11:46:40Z","timestamp":1782215200195,"version":"3.54.5"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:00:00Z","timestamp":1704672000000},"content-version":"vor","delay-in-days":47,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003841","name":"Ilam University of Medical Sciences","doi-asserted-by":"publisher","award":["A-10-3546-3"],"award-info":[{"award-number":["A-10-3546-3"]}],"id":[{"id":"10.13039\/501100003841","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The rising issue of antibiotic resistance has made treating Pseudomonas aeruginosa infections increasingly challenging. Therefore, vaccines have emerged as a viable alternative to antibiotics for preventing P. aeruginosa infections in susceptible individuals. With its superior accuracy, high efficiency in stimulating cellular and humoral immune responses, and low cost, mRNA vaccine technology is quickly replacing traditional methods. This study aimed to design a novel mRNA vaccine by using in silico approaches against P. aeruginosa. The research team identified five surface and antigenic proteins and selected their appropriate epitopes with immunoinformatic tools. These epitopes were then examined for toxicity, allergenicity and homology. The researchers also checked their presentation and identification by major histocompatibility complex cells and other immune cells through valuable tools like molecular docking. They subsequently modeled a multi-epitope protein and optimized it. The mRNA was analyzed in terms of structure and stability, after which the immune system\u2019s response against the new vaccine was simulated. The results indicated that the designed mRNA construct could be an effective and promising vaccine that requires laboratory and clinical trials.<\/jats:p>","DOI":"10.1093\/bib\/bbad502","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T11:08:33Z","timestamp":1704712113000},"source":"Crossref","is-referenced-by-count":21,"title":["Development of innovative multi-epitope mRNA vaccine against <i>Pseudomonas aeruginosa<\/i> using <i>in silico<\/i> approaches"],"prefix":"10.1093","volume":"25","author":[{"given":"Maryam","family":"Asadinezhad","sequence":"first","affiliation":[{"name":"Students Research Committee, Ilam University of Medical Sciences , Ilam , Iran"},{"name":"Department of Microbiology , Faculty of Medicine, , Ilam , Iran"},{"name":"Ilam University of Medical Sciences , Faculty of Medicine, , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5143-3178","authenticated-orcid":false,"given":"Saeed","family":"Khoshnood","sequence":"additional","affiliation":[{"name":"Students Research Committee, Ilam University of Medical Sciences , Ilam , Iran"},{"name":"Clinical Microbiology Research Center, Ilam University of Medical Sciences , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parisa","family":"Asadollahi","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Faculty of Medicine, , Ilam , Iran"},{"name":"Ilam University of Medical Sciences , Faculty of Medicine, , Ilam , Iran"},{"name":"Clinical Microbiology Research Center, Ilam University of Medical Sciences , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sobhan","family":"Ghafourian","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Faculty of Medicine, , Ilam , Iran"},{"name":"Ilam University of Medical Sciences , Faculty of Medicine, , Ilam , Iran"},{"name":"Clinical Microbiology Research Center, Ilam University of Medical Sciences , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nourkhoda","family":"Sadeghifard","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Faculty of Medicine, , Ilam , Iran"},{"name":"Ilam University of Medical Sciences , Faculty of Medicine, , Ilam , Iran"},{"name":"Clinical Microbiology Research Center, Ilam University of Medical Sciences , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iraj","family":"Pakzad","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Faculty of Medicine, , Ilam , Iran"},{"name":"Ilam University of Medical Sciences , Faculty of Medicine, , Ilam , Iran"},{"name":"Clinical Microbiology Research Center, Ilam University of Medical Sciences , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasaman","family":"Zeinivand","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Faculty of Medicine, , Ilam , Iran"},{"name":"Ilam University of Medical Sciences , Faculty of Medicine, , Ilam , Iran"},{"name":"Clinical Microbiology Research Center, Ilam University of Medical Sciences , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazanin","family":"Omidi","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Faculty of Medicine, , Ilam , Iran"},{"name":"Ilam University of Medical Sciences , Faculty of Medicine, , Ilam , Iran"},{"name":"Clinical Microbiology Research Center, Ilam University of Medical Sciences , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Hematian","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Faculty of Medicine, , Ilam , Iran"},{"name":"Ilam University of Medical Sciences , Faculty of Medicine, , Ilam , Iran"},{"name":"Clinical Microbiology Research Center, Ilam University of Medical Sciences , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behrooz Sadeghi","family":"Kalani","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Faculty of Medicine, , Ilam , Iran"},{"name":"Ilam University of Medical Sciences , Faculty of Medicine, , Ilam , Iran"},{"name":"Clinical Microbiology Research Center, Ilam University of Medical Sciences , Ilam , Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,6]]},"reference":[{"key":"2024010811054978200_ref1","doi-asserted-by":"crossref","first-page":"583008","DOI":"10.3389\/fimmu.2020.583008","article-title":"Development of a broadly protective, self-adjuvanting subunit vaccine to prevent infections by Pseudomonas 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