{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:26:22Z","timestamp":1780403182008,"version":"3.54.1"},"reference-count":62,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,1,27]],"date-time":"2024-01-27T00:00:00Z","timestamp":1706313600000},"content-version":"vor","delay-in-days":5,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["18\/23693-5"],"award-info":[{"award-number":["18\/23693-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["22\/11192-7"],"award-info":[{"award-number":["22\/11192-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["20\/02976-9"],"award-info":[{"award-number":["20\/02976-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cleavage Under Targets and Release Using Nuclease (CUT&amp;RUN) is a recent development for epigenome mapping, but its unique methodology can hamper proper quantitative analyses. As traditional normalization approaches have been shown to be inaccurate, we sought to determine endogenous normalization factors based on the human genome regions of constant nonspecific signal. This constancy was determined by applying Shannon\u2019s information entropy, and the set of normalizer regions, which we named the \u2018Greenlist\u2019, was extensively validated using publicly available datasets. We demonstrate here that the greenlist normalization outperforms the current top standards, and remains consistent across different experimental setups, cell lines and antibodies; the approach can even be applied to different species or to CUT&amp;Tag. Requiring no additional experimental steps and no added cost, this approach can be universally applied to CUT&amp;RUN experiments to greatly minimize the interference of technical variation over the biological epigenome changes of interest.<\/jats:p>","DOI":"10.1093\/bib\/bbad538","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T18:38:57Z","timestamp":1703702337000},"source":"Crossref","is-referenced-by-count":17,"title":["The CUT&amp;RUN greenlist: genomic regions of consistent noise are effective normalizing factors for quantitative epigenome mapping"],"prefix":"10.1093","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0678-3707","authenticated-orcid":false,"given":"Fabio N","family":"de Mello","sequence":"first","affiliation":[{"name":"Cell Cycle Laboratory, Instituto Butantan , S\u00e3o Paulo , Brazil"},{"name":"Interunit Bioinformatics Graduate Program, Universidade de S\u00e3o Paulo , S\u00e3o Paulo , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C","family":"Tahira","sequence":"additional","affiliation":[{"name":"Cell Cycle Laboratory, Instituto Butantan , S\u00e3o Paulo , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Gabriela","family":"Berzoti-Coelho","sequence":"additional","affiliation":[{"name":"Cell Cycle Laboratory, Instituto Butantan , S\u00e3o Paulo , Brazil"},{"name":"Instituto de Qu\u00edmica, Universidade de S\u00e3o Paulo , S\u00e3o Paulo , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6356-2401","authenticated-orcid":false,"given":"Sergio","family":"Verjovski-Almeida","sequence":"additional","affiliation":[{"name":"Cell Cycle Laboratory, Instituto Butantan , S\u00e3o Paulo , Brazil"},{"name":"Instituto de Qu\u00edmica, Universidade de S\u00e3o Paulo , S\u00e3o Paulo , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,26]]},"reference":[{"issue":"5830","key":"2024012709100420000_ref1","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1126\/science.1141319","article-title":"Genome-wide mapping of in vivo protein-DNA interactions","volume":"316","author":"Johnson","year":"2007","journal-title":"Science"},{"issue":"10","key":"2024012709100420000_ref2","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1038\/nrg2641","article-title":"ChIP-seq: advantages and challenges of a maturing technology","volume":"10","author":"Park","year":"2009","journal-title":"Nat Rev Genet"},{"issue":"12","key":"2024012709100420000_ref3","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1038\/nrg3306","article-title":"ChIP-seq and beyond: new and improved methodologies to detect and characterize protein-DNA interactions","volume":"13","author":"Furey","year":"2012","journal-title":"Nat Rev Genet"},{"key":"2024012709100420000_ref4","doi-asserted-by":"crossref","first-page":"e21856","DOI":"10.7554\/eLife.21856","article-title":"An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites","volume":"6","author":"Skene","year":"2017","journal-title":"Elife"},{"issue":"1","key":"2024012709100420000_ref5","first-page":"147","article-title":"ChIC and ChEC; genomic mapping of chromatin proteins","volume":"16","author":"Schmid","year":"2004","journal-title":"Mol Cell"},{"key":"2024012709100420000_ref6","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.46314","article-title":"Improved CUT&RUN chromatin profiling tools","volume":"8","author":"Meers","year":"2019","journal-title":"Elife"},{"issue":"1","key":"2024012709100420000_ref7","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s13072-019-0287-4","article-title":"Peak calling by sparse enrichment analysis for CUT&RUN chromatin profiling","volume":"12","author":"Meers","year":"2019","journal-title":"Epigenetics Chromatin"},{"key":"2024012709100420000_ref8","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcmd.2023.102745","article-title":"High-throughput methods for the analysis of transcription factors and chromatin modifications: low input, single cell and spatial genomic technologies","volume":"101","author":"Salma","year":"2023","journal-title":"Blood Cells Mol Dis"},{"issue":"1","key":"2024012709100420000_ref9","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s10577-019-09619-9","article-title":"Genomic methods in profiling DNA accessibility and factor localization","volume":"28","author":"Klein","year":"2020","journal-title":"Chromosome Res"},{"issue":"16","key":"2024012709100420000_ref10","doi-asserted-by":"crossref","first-page":"8809","DOI":"10.3390\/ijms22168809","article-title":"Emerging single-cell technological approaches to investigate chromatin dynamics and centromere regulation in human health and disease","volume":"22","author":"Leo","year":"2021","journal-title":"Int J Mol Sci"},{"key":"2024012709100420000_ref11","doi-asserted-by":"crossref","first-page":"e13773","DOI":"10.1111\/acel.13773","article-title":"STAT3 promotes a youthful epigenetic state in articular chondrocytes","volume":"22","author":"Sarkar","year":"2023","journal-title":"Aging Cell"},{"key":"2024012709100420000_ref12","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1038\/s41594-022-00880-x","article-title":"Cooperation of chromatin remodeling SWI\/SNF complex and pioneer factor AP-1 shapes 3D enhancer landscapes","volume":"30","author":"Wolf","year":"2022","journal-title":"Nat Struct Mol Biol"},{"key":"2024012709100420000_ref13","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1080\/10985549.2023.2229225","article-title":"Shared gene targets of the ATF4 and p53 transcriptional networks","volume":"43","author":"Baniulyte","year":"2023","journal-title":"Mol Cell Biol"},{"issue":"1","key":"2024012709100420000_ref14","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1038\/s41467-019-09982-5","article-title":"CUT&tag for efficient epigenomic profiling of small samples and single cells","volume":"10","author":"Kaya-Okur","year":"2019","journal-title":"Nat Commun"},{"key":"2024012709100420000_ref15","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1038\/s41594-023-01092-7","article-title":"Transcriptional repression upon S phase entry protects genome integrity in pluripotent cells","volume":"30","author":"G\u00f6kbuget","year":"2023","journal-title":"Nat Struct Mol Biol"},{"key":"2024012709100420000_ref16","first-page":"285","article-title":"The ETS transcription factor ETV6 constrains the transcriptional activity of EWS\u2013FLI to promote Ewing sarcoma","volume":"25","author":"Lu","year":"2023","journal-title":"Nat Cell Biol"},{"key":"2024012709100420000_ref17","doi-asserted-by":"crossref","first-page":"4940","DOI":"10.1038\/s41467-020-18761-6","article-title":"Tasor is a pseudo-PARP that directs hush complex assembly and epigenetic transposon control","volume":"11","author":"Douse","year":"2020","journal-title":"Nat Commun"},{"key":"2024012709100420000_ref18","article-title":"Scalable single-cell profiling of chromatin modifications with sciCUT&Tag","volume":"19","author":"Janssens","journal-title":"Nat Prot"},{"key":"2024012709100420000_ref19","doi-asserted-by":"crossref","first-page":"2633","DOI":"10.1038\/s41596-021-00516-2","article-title":"Transcription factor chromatin profiling genome-wide using ulicut&run in single cells and individual blastocysts","volume":"16","author":"Patty","year":"2021","journal-title":"Nat Protoc"},{"key":"2024012709100420000_ref20","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1038\/s41587-021-00869-9","article-title":"Single-cell CUT&TAG profiles histone modifications and transcription factors in complex tissues","volume":"39","author":"Bartosovic","year":"2021","journal-title":"Nat Biotech"},{"key":"2024012709100420000_ref21","doi-asserted-by":"crossref","first-page":"dev201124","DOI":"10.1242\/dev.201124","article-title":"A new CUT&RUN low volume-urea (LOV-U) protocol optimized for transcriptional co-factors uncovers WNT\/\u03b2-catenin tissue-specific genomic targets","volume":"149","author":"Zambanini","year":"2022","journal-title":"Development"},{"key":"2024012709100420000_ref22","doi-asserted-by":"crossref","DOI":"10.1002\/cpz1.266","article-title":"GreenCUT&RUN: efficient genomic profiling of gfp-tagged transcription factors and chromatin regulators","volume":"1","author":"Koidl","year":"2021","journal-title":"Curr Protoc"},{"key":"2024012709100420000_ref23","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s13059-022-02642-w","article-title":"CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells","volume":"23","author":"Janssens","year":"2022","journal-title":"Genome Biol"},{"key":"2024012709100420000_ref24","doi-asserted-by":"crossref","DOI":"10.17504\/protocols.io.x54v9mkmzg3e\/v4","article-title":"CUT&Tag-Direct for Whole Cells with CUTAC V2","author":"Henikoff","year":"2023"},{"issue":"3","key":"2024012709100420000_ref25","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.molcel.2019.05.025","article-title":"Pioneer factor-nucleosome binding events during differentiation are motif encoded","volume":"75","author":"Meers","year":"2019","journal-title":"Mol Cell"},{"issue":"1","key":"2024012709100420000_ref26","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/bioinformatics\/btab507","article-title":"CUT&RUNTools 2.0: a pipeline for single-cell and bulk-level CUT&RUN and CUT&Tag data analysis","volume":"38","author":"Yu","year":"2021","journal-title":"Bioinformatics"},{"issue":"1","key":"2024012709100420000_ref27","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1186\/s13104-021-05781-8","article-title":"ssvQC: an integrated CUT&RUN quality control workflow for histone modifications and transcription factors","volume":"14","author":"Boyd","year":"2021","journal-title":"BMC Res Notes"},{"issue":"3","key":"2024012709100420000_ref28","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1016\/j.celrep.2014.10.018","article-title":"Quantitative ChIP-Seq normalization reveals global modulation of the epigenome","volume":"9","author":"Orlando","year":"2014","journal-title":"Cell Rep"},{"issue":"2","key":"2024012709100420000_ref29","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3390\/genes1020317","article-title":"Statistical issues in the analysis of ChIP-Seq and RNA-Seq data","volume":"1","author":"Ghosh","year":"2010","journal-title":"Genes (Basel)"},{"issue":"47","key":"2024012709100420000_ref30","doi-asserted-by":"crossref","first-page":"15826","DOI":"10.1074\/jbc.RA120.015353","article-title":"A physical basis for quantitative ChIP-sequencing","volume":"295","author":"Dickson","year":"2020","journal-title":"J Biol Chem"},{"issue":"5","key":"2024012709100420000_ref31","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1128\/MCB.00970-14","article-title":"The overlooked fact: fundamental need for spike-in control for virtually all genome-wide analyses","volume":"36","author":"Chen","year":"2015","journal-title":"Mol Cell Biol"},{"issue":"5","key":"2024012709100420000_ref32","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1016\/j.molcel.2015.04.022","article-title":"Calibrating ChIP-Seq with nucleosomal internal standards to measure histone modification density genome wide","volume":"58","author":"Grzybowski","year":"2015","journal-title":"Mol Cell"},{"issue":"7","key":"2024012709100420000_ref33","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1101\/gr.168260.113","article-title":"Quantifying ChIP-seq data: a spiking method providing an internal reference for sample-to-sample normalization","volume":"24","author":"Bonhoure","year":"2014","journal-title":"Genome Res"},{"issue":"1","key":"2024012709100420000_ref34","doi-asserted-by":"crossref","first-page":"9354","DOI":"10.1038\/s41598-019-45839-z","article-title":"The ENCODE blacklist: identification of problematic regions of the genome","volume":"9","author":"Amemiya","year":"2019","journal-title":"Sci Rep"},{"key":"2024012709100420000_ref35","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3389\/fgene.2014.00075","article-title":"Impact of artifact removal on ChIP quality metrics in ChIP-seq and ChIP-exo data","volume":"5","author":"Carroll","year":"2014","journal-title":"Front Genet"},{"issue":"2","key":"2024012709100420000_ref36","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1089\/cmb.2019.0295","article-title":"PeakPass: automating ChIP-Seq blacklist creation","volume":"27","author":"Wimberley","year":"2020","journal-title":"J Comput Biol"},{"key":"2024012709100420000_ref37","volume-title":"DiffBind: Differential Binding Analysis of ChIP-Seq Peak Data","author":"Stark","year":"2011"},{"issue":"4","key":"2024012709100420000_ref38","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1002\/j.1538-7305.1948.tb00917.x","article-title":"A mathematical theory of communication","volume":"27","author":"Shannon","year":"1948","journal-title":"Bell Syst Tech J"},{"key":"2024012709100420000_ref39","first-page":"1469","article-title":"Entropy inference and the James-Stein estimator, with application to nonlinear gene association networks","volume":"10","author":"Hausser","year":"2009","journal-title":"J Mach Learn Res"},{"key":"2024012709100420000_ref40","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1101\/gr.275472.121","article-title":"A chip-exo screen of 887 protein capture reagents program transcription factor antibodies in human cells","volume":"31","author":"Lai","year":"2021","journal-title":"Genome Res"},{"key":"2024012709100420000_ref41","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1186\/s13059-023-03027-3","article-title":"The CUT&RUN suspect list of problematic regions of the genome","volume":"24","author":"Nordin","year":"2023","journal-title":"Genome Biol"},{"issue":"5","key":"2024012709100420000_ref42","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1038\/nprot.2018.015","article-title":"Targeted in situ genome-wide profiling with high efficiency for low cell numbers","volume":"13","author":"Skene","year":"2018","journal-title":"Nat Protoc"},{"key":"2024012709100420000_ref43","doi-asserted-by":"crossref","first-page":"eabq2096","DOI":"10.1126\/scitranslmed.abq2096","article-title":"Targeting KDM4 for treating Pax3-FOXO1\u2013driven alveolar rhabdomyosarcoma","volume":"14","author":"Singh","year":"2022","journal-title":"Sci Transl Med"},{"issue":"12","key":"2024012709100420000_ref44","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"issue":"14","key":"2024012709100420000_ref45","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1182\/blood.2022016528","article-title":"HOXA9 forms a repressive complex with nuclear matrix-associated protein SAFB to maintain acute myeloid leukemia","volume":"141","author":"Agrawal-Singh","year":"2023","journal-title":"Blood"},{"issue":"3","key":"2024012709100420000_ref46","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41556-023-01093-0","article-title":"A neurodevelopmental epigenetic programme mediated by SMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastoma metastasis","volume":"25","author":"Zou","year":"2023","journal-title":"Nat Cell Biol"},{"issue":"7979","key":"2024012709100420000_ref47","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1038\/s41586-023-06515-5","article-title":"R-loop-dependent promoter-proximal termination ensures genome stability","volume":"621","author":"Xu","year":"2023","journal-title":"Nature"},{"issue":"5","key":"2024012709100420000_ref48","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1038\/s41380-023-01959-7","article-title":"MYT1L haploinsufficiency in human neurons and mice causes autism-associated phenotypes that can be reversed by genetic and pharmacologic intervention","volume":"28","author":"Weigel","year":"2023","journal-title":"Mol Psy"},{"issue":"5","key":"2024012709100420000_ref49","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1038\/s41588-021-00843-w","article-title":"ZNF410 represses fetal globin by singular control of CHD4","volume":"53","author":"Vinjamur","year":"2021","journal-title":"Nat Genetics"},{"issue":"3","key":"2024012709100420000_ref50","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2021.100750","article-title":"A modified CUT&RUN protocol and analysis pipeline to identify transcription factor binding sites in human cell lines","volume":"2","author":"Kong","year":"2021","journal-title":"STAR Protoc"},{"key":"2024012709100420000_ref51","volume-title":"EpiCypher"},{"key":"2024012709100420000_ref52","volume-title":"Cell Signaling Technology"},{"issue":"17","key":"2024012709100420000_ref53","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"issue":"4","key":"2024012709100420000_ref54","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat Methods"},{"issue":"D1","key":"2024012709100420000_ref55","doi-asserted-by":"crossref","first-page":"D916","DOI":"10.1093\/nar\/gkaa1087","article-title":"Gencode 2021","volume":"49","author":"Frankish","year":"2021","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"2024012709100420000_ref56","doi-asserted-by":"crossref","first-page":"giab008","DOI":"10.1093\/gigascience\/giab008","article-title":"Twelve years of SAMtools and BCFtools","volume":"10","author":"Danecek","year":"2021","journal-title":"Gigascience"},{"issue":"6","key":"2024012709100420000_ref57","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: a flexible suite of utilities for comparing genomic features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"issue":"W1","key":"2024012709100420000_ref58","doi-asserted-by":"crossref","first-page":"W160","DOI":"10.1093\/nar\/gkw257","article-title":"deepTools2: a next generation web server for deep-sequencing data analysis","volume":"44","author":"Ramirez","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"2024012709100420000_ref59","volume-title":"kbroman\/broman: Version 0.80 (0.80) Zenodo","author":"Broman","year":"2022"},{"key":"2024012709100420000_ref60","volume-title":"An R Companion to Applied Regression","author":"Fox","year":"2019","edition":"3rd ed"},{"key":"2024012709100420000_ref61","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2021"},{"key":"2024012709100420000_ref62","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham","year":"2016","edition":"2nd"}],"container-title":["Briefings in Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bib\/article-pdf\/25\/2\/bbad538\/56428241\/bbad538.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bib\/article-pdf\/25\/2\/bbad538\/56428241\/bbad538.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,27]],"date-time":"2024-01-27T04:10:44Z","timestamp":1706328644000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bib\/article\/doi\/10.1093\/bib\/bbad538\/7590321"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,22]]},"references-count":62,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,1,22]]}},"URL":"https:\/\/doi.org\/10.1093\/bib\/bbad538","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.10.26.564165","asserted-by":"object"}]},"ISSN":["1467-5463","1477-4054"],"issn-type":[{"value":"1467-5463","type":"print"},{"value":"1477-4054","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,3,1]]},"published":{"date-parts":[[2024,1,22]]},"article-number":"bbad538"}}