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Despite the tolerable prediction performance, the application scope of current approaches is limited to the prediction at the species level, which cannot accurately predict the relationship of phages across strain mutants. This has hindered the development of phage therapeutics based on the prediction of phage\u2013bacteria relationships. In this paper, we present, PB-LKS, to predict the phage\u2013bacteria interaction based on local K-mer strategy with higher performance and wider applicability. The utility of PB-LKS is rigorously validated through (i) large-scale historical screening, (ii) case study at the class level and (iii) in vitro simulation of bacterial antiphage resistance at the strain mutant level. The PB-LKS approach could outperform the current state-of-the-art methods and illustrate potential clinical utility in pre-optimized phage therapy design.<\/jats:p>","DOI":"10.1093\/bib\/bbae010","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T08:35:46Z","timestamp":1707726946000},"source":"Crossref","is-referenced-by-count":14,"title":["PB-LKS: a python package for predicting phage\u2013bacteria interaction through local K-mer strategy"],"prefix":"10.1093","volume":"25","author":[{"given":"Jingxuan","family":"Qiu","sequence":"first","affiliation":[{"name":"School of Health Science and Engineering, University of Shanghai for Science and Technology , Shanghai, 200093, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanchun","family":"Nie","sequence":"additional","affiliation":[{"name":"School of Health Science and Engineering, University of Shanghai for Science and Technology , Shanghai, 200093, 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