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Here, we report IMPRINTS.CETSA, an R package that provides the basic data processing framework for robust analysis of the IMPRINTS-CETSA data format, from preprocessing and normalization to visualization. We also report an accompanying R package, IMPRINTS.CETSA.app, which offers a user-friendly Shiny interface for analysis and interpretation of IMPRINTS-CETSA results, with seamless features such as functional enrichment and mapping to other databases at a single site. For the hit generation part, the diverse behaviors of protein modulations have been typically segregated with a two-measure scoring method, i.e. the abundance and thermal stability changes. We present a new algorithm to classify modulated proteins in IMPRINTS-CETSA experiments by a robust single-measure scoring. In this way, both the numerical changes and the statistical significances of the IMPRINTS information can be visualized on a single plot. The IMPRINTS.CETSA and IMPRINTS.CETSA.app R packages are freely available on GitHub at https:\/\/github.com\/nkdailingyun\/IMPRINTS.CETSA and https:\/\/github.com\/mgerault\/IMPRINTS.CETSA.app, respectively. IMPRINTS.CETSA.app is also available as an executable program at https:\/\/zenodo.org\/records\/10636134.<\/jats:p>","DOI":"10.1093\/bib\/bbae128","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T09:38:11Z","timestamp":1711964291000},"source":"Crossref","is-referenced-by-count":8,"title":["IMPRINTS.CETSA and IMPRINTS.CETSA.app: an R package and a Shiny application for the analysis and interpretation of IMPRINTS-CETSA data"],"prefix":"10.1093","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7430-3467","authenticated-orcid":false,"given":"Marc-Antoine","family":"Gerault","sequence":"first","affiliation":[{"name":"Department of Oncology and Pathology, Karolinska Institutet , 171 77 Stockholm , Sweden"},{"name":"Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Prot\u00e9omique , F-13009 Marseille , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Granjeaud","sequence":"additional","affiliation":[{"name":"Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Prot\u00e9omique , F-13009 Marseille , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc","family":"Camoin","sequence":"additional","affiliation":[{"name":"Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Prot\u00e9omique , F-13009 Marseille , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u00e4r","family":"Nordlund","sequence":"additional","affiliation":[{"name":"Department of Oncology and Pathology, Karolinska Institutet , 171 77 Stockholm , Sweden"},{"name":"Institute of Molecular and Cell Biology, A*STAR , 138673 , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9386-5967","authenticated-orcid":false,"given":"Lingyun","family":"Dai","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology, A*STAR , 138673 , Singapore"},{"name":"Department of Geriatrics , and Shenzhen Clinical Research Centre for Geriatrics, , Shenzhen 518020 , China"},{"name":"The First Affiliated Hospital, School of Medicine, Southern University of Science and Technology , and Shenzhen Clinical Research Centre for Geriatrics, , Shenzhen 518020 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,3,31]]},"reference":[{"issue":"2","key":"2024040109375795900_ref1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/0022-5193(65)90083-4","article-title":"Molecules as documents of evolutionary history","volume":"8","author":"Zuckerkandl","year":"1965","journal-title":"J 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