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Herein, we developed a diffusion model to learn features from different types of human collagens and generate CMPs; obtaining 66% of synthetic CMPs could self-assemble into triple helices. Triple-helical and untwisting states were probed by melting temperature (Tm); hence, we developed a model to predict collagen Tm, achieving a state-of-art Pearson\u2019s correlation (PC) of 0.95 by cross-validation and a PC of 0.8 for predicting Tm values of synthetic CMPs. Our chemically synthesized short CMPs and recombinantly expressed long CMPs could self-assemble, with the lowest requirement for hydrogel formation at a concentration of 0.08% (w\/v). Five CMPs could promote osteoblast differentiation. Our results demonstrated the potential for using computer-aided methods to design functional self-assembling CMPs.<\/jats:p>","DOI":"10.1093\/bib\/bbae622","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T12:48:25Z","timestamp":1731588505000},"source":"Crossref","is-referenced-by-count":8,"title":["Diffusion model assisted designing self-assembling collagen mimetic peptides as biocompatible materials"],"prefix":"10.1093","volume":"26","author":[{"given":"Xinglong","family":"Wang","sequence":"first","affiliation":[{"name":"Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology and School of Biotechnology, Jiangnan University , 1800 Lihu Road, Wuxi, Jiangsu 214222 ,","place":["China"]},{"name":"Science Center for Future Foods, Jiangnan University , 1800 Lihu Road, Wuxi, Jiangsu 214222 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Jiangnan University , 1800 Lihu Road, Wuxi, Jiangsu 214222 ,","place":["China"]},{"name":"Science Center for Future Foods, Jiangnan University , 1800 Lihu Road, Wuxi, Jiangsu 214222 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyao","family":"Yu","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology and School of Biotechnology, Jiangnan University , 1800 Lihu Road, Wuxi, Jiangsu 214222 ,","place":["China"]},{"name":"Science Center for Future Foods, Jiangnan University , 1800 Lihu Road, Wuxi, Jiangsu 214222 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3949-3733","authenticated-orcid":false,"given":"Jingwen","family":"Zhou","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology and School of Biotechnology, Jiangnan 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