{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:29:03Z","timestamp":1784543343050,"version":"3.55.0"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T00:00:00Z","timestamp":1735776000000},"content-version":"vor","delay-in-days":41,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Centre on High-Performance Computing, Big Data and Quantum Computing","award":["CN_00000013"],"award-info":[{"award-number":["CN_00000013"]}]},{"name":"Complementary National Plan PNC-I.1","award":["PNC0000002"],"award-info":[{"award-number":["PNC0000002"]}]},{"name":"Complementary National Plan PNC-I.1","award":["PE_0000007"],"award-info":[{"award-number":["PE_0000007"]}]},{"name":"Life Science Hub Puglia","award":["CUP H93C22000560003"],"award-info":[{"award-number":["CUP H93C22000560003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The advent of high-throughput sequencing (HTS) technologies unlocked the complexity of the microbial world through the development of metagenomics, which now provides an unprecedented and comprehensive overview of its taxonomic and functional contribution in a huge variety of macro- and micro-ecosystems. In particular, shotgun metagenomics allows the reconstruction of microbial genomes, through the assembly of reads into MAGs (metagenome-assembled genomes). In fact, MAGs represent an information-rich proxy for inferring the taxonomic composition and the functional contribution of microbiomes, even if the relevant analytical approaches are not trivial and still improvable. In this regard, tools like CAMITAX and GTDBtk have implemented complex approaches, relying on marker gene identification and sequence alignments, requiring a large processing time. With the aim of deploying an effective tool for fast and reliable MAG taxonomic classification, we present here kMetaShot, a taxonomy classifier based on k-mer\/minimizer counting. We benchmarked kMetaShot against CAMITAX and GTDBtk by using both in silico and real mock communities and demonstrated how, while implementing a fast and concise algorithm, it outperforms the other tools in terms of classification accuracy. Additionally, kMetaShot is an easy-to-install and easy-to-use bioinformatic tool that is also suitable for researchers with few command-line skills. It is available and documented at https:\/\/github.com\/gdefazio\/kMetaShot.<\/jats:p>","DOI":"10.1093\/bib\/bbae680","type":"journal-article","created":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T09:46:36Z","timestamp":1735897596000},"source":"Crossref","is-referenced-by-count":8,"title":["kMetaShot: a fast and reliable taxonomy classifier for metagenome-assembled genomes"],"prefix":"10.1093","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9356-5224","authenticated-orcid":false,"given":"Giuseppe","family":"Defazio","sequence":"first","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro , Via E. Orabona 4, 70126, Bari ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3923-2266","authenticated-orcid":false,"given":"Marco Antonio","family":"Tangaro","sequence":"additional","affiliation":[{"name":"Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, Consiglio Nazionale delle Ricerche , Via G. Amendola 122\/O, 70125, Bari ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3663-0859","authenticated-orcid":false,"given":"Graziano","family":"Pesole","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro , Via E. Orabona 4, 70126, Bari ,","place":["Italy"]},{"name":"Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, Consiglio Nazionale delle Ricerche , Via G. Amendola 122\/O, 70125, Bari ,","place":["Italy"]},{"name":"Consorzio Interuniversitario Biotecnologie , BIC Incubatori, Via Flavia 23\/1, 34148, Trieste ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2324-086X","authenticated-orcid":false,"given":"Bruno","family":"Fosso","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro , Via E. 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