{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T06:10:38Z","timestamp":1783491038728,"version":"3.55.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":61,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007567","name":"City University of Hong Kong","doi-asserted-by":"publisher","award":["11209823"],"award-info":[{"award-number":["11209823"]}],"id":[{"id":"10.13039\/100007567","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Bacteriophages are viruses that target bacteria, playing a crucial role in microbial ecology. Phage proteins are important in understanding phage biology, such as virus infection, replication, and evolution. Although a large number of new phages have been identified via metagenomic sequencing, many of them have limited protein function annotation. Accurate function annotation of phage proteins presents several challenges, including their inherent diversity and the scarcity of annotated ones. Existing tools have yet to fully leverage the unique properties of phages in annotating protein functions. In this work, we propose a new protein function annotation tool for phages by leveraging the modular genomic structure of phage genomes. By employing embeddings from the latest protein foundation models and Transformer to capture contextual information between proteins in phage genomes, GOPhage surpasses state-of-the-art methods in annotating diverged proteins and proteins with uncommon functions by 6.78% and 13.05% improvement, respectively. GOPhage can annotate proteins lacking homology search results, which is critical for characterizing the rapidly accumulating phage genomes. We demonstrate the utility of GOPhage by identifying 688 potential holins in phages, which exhibit high structural conservation with known holins. The results show the potential of GOPhage to extend our understanding of newly discovered phages.<\/jats:p>","DOI":"10.1093\/bib\/bbaf014","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T07:39:01Z","timestamp":1737531541000},"source":"Crossref","is-referenced-by-count":8,"title":["GOPhage: protein function annotation for bacteriophages by integrating the genomic context"],"prefix":"10.1093","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9200-4862","authenticated-orcid":false,"given":"Jiaojiao","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Electrical Engineering, City University of Hong Kong , 83 Tat Chee Ave, Kowloon Tong, Hong Kong (SAR),","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5974-4985","authenticated-orcid":false,"given":"Yongxin","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, City University of Hong Kong , 83 Tat Chee Ave, Kowloon Tong, Hong Kong (SAR),","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8566-0707","authenticated-orcid":false,"given":"Cheng","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, City University of Hong Kong , 83 Tat Chee Ave, Kowloon Tong, Hong Kong (SAR),","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2559-2519","authenticated-orcid":false,"given":"Wei","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, City University of Hong Kong , 83 Tat Chee Ave, Kowloon Tong, Hong Kong (SAR),","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xubo","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, City University of 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