{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T19:46:26Z","timestamp":1784317586790,"version":"3.55.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":6,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024M763546"],"award-info":[{"award-number":["2024M763546"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Characterizing the binding interactions between major histocompatibility complex (MHC) class II molecules and peptides is crucial for studying the immune system, offering potential applications for neoantigen design, vaccine development, and personalized immunotherapy. Motivated by this profound meaning, we developed a model that integrates large language models (LLMs) and deep hypergraph learning for predicting MHC class II\u2013peptide binding reactivity, affinity, and residue contact profiling. pMHChat takes MHC pseudo-sequences and peptide sequences as inputs and processes them through four stages: LLMs fine-tune stage, feature encoding and map fusion stage, task-specific prediction stage, and downstream analysis stage. pMHChat distinguishes itself in capturing contextually relevant and high-order spatial interactions of the peptide\u2013MHC (pMHC) complex. Specifically, in a five-fold cross-validation experiment, pMHChat achieves superior performance, with a mean area under the receiver operating characteristic curve of 0.8744 and an area under the precision\u2013recall curve of 0.8390 in the binding reactivity task, as well as a mean Pearson correlation coefficient of 0.7311 in the binding affinity prediction task. Furthermore, pMHChat also demonstrates the best performance in both the leave-one-molecule-out setting and independent evaluation. Notably, pMHChat can provide residue contact profiling, showing its potential application in recognizing critical binding patterns of the pMHC complex. Our findings highlight pMHChat\u2019s capacity to advance both predictive accuracy and detailed insights into the MHC\u2013peptide binding process. We anticipate that pMHChat will serve as a powerful tool for elucidating MHC\u2013peptide interactions, with promising applications in immunological research and therapeutic development.<\/jats:p>","DOI":"10.1093\/bib\/bbaf321","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T12:00:46Z","timestamp":1750420846000},"source":"Crossref","is-referenced-by-count":4,"title":["pMHChat, characterizing the interactions between major histocompatibility complex class II molecules and peptides with large language models and deep hypergraph learning"],"prefix":"10.1093","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8126-0431","authenticated-orcid":false,"given":"Jiani","family":"Ma","sequence":"first","affiliation":[{"name":"School of Information and Control Engineering, China University of Mining and Technology , No. 1 Daxue Road, Tongshan District, Xuzhou, Jiangsu 221116 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