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However, significant knowledge gaps persist regarding its functional implications, sequence motifs, and structural characteristics. Through integrated functional, sequential, and structural analyses of experimentally verified large-scale N-phosphorylation datasets, we found that protein N-phosphorylation broadly participates in diverse biological processes and exhibits close associations with human diseases, particularly cancer. And the high motif conservation between N- and O-phosphorylation in eukaryotes offers novel research perspectives for investigating N-phosphorylation through the lens of O-phosphorylation. Moreover, the structural-sequence pattern analyses demonstrate that relative solvent accessibility at modification sites provides more distinctive characterization of phosphorylation sites than other structural-sequence descriptors. 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