{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T21:59:35Z","timestamp":1766786375704,"version":"3.48.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"Supplement_1","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":11,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Glioblastoma (GBM) exhibits pronounced cellular plasticity that contributes to therapeutic resistance. A facet of this adaptability is neuronal mimicry, whereby tumor cells acquire neuron-like features to engage with neural circuits. The coherence and biological significance of this process remain uncertain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Spatial transcriptomic and proteomic datasets from Greenwald et al. [1] were analyzed alongside healthy cortical tissue from Ravi et al. [2] as a non-malignant reference. A permutation-based framework identified tumor\u2013neuronal (TN) cells across Healthy, Core, Infiltrative, and Bulk regions, with stability verified by resampling and Jaccard overlap (CV\u2009&amp;lt;\u200910%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>TN cells were enriched in Bulk (OR \u2248 70, p\u2009&amp;lt;\u200910\u221210) and Infiltrative (OR \u2248 7, p\u2009&amp;lt;\u200910\u221210) regions relative to Healthy. Integrative analyses revealed a synaptic-like transcriptional program marked by SNAP25, SYT1, and STMN2 (logFC \u2248 1\u20131.5), enriched for vesicle cycling and cytoskeletal remodeling pathways. Neuronal subtype markers (SLC17A7, GABRA1) were depleted, while malignant markers (CLU, GFAP, VIM) persisted. Single-cell proteomics confirmed co-localization of neuronal and tumor proteins within clustered, double-positive cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study delineates a reproducible hybrid state with partial neuronal characteristics, suggesting that neuronal mimicry represents an adaptive, rather than lineage-converted, trajectory of GBM that may inform strategies to disrupt tumor\u2013neuron crosstalk.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>References<\/jats:title>\n                    <jats:p>1. Greenwald AC, et al. \u2018Integrative spatial analysis reveals a multi-layered organization of glioblastoma.\u2019 Cell 2024;187(10):2485\u20132501.e26.<\/jats:p>\n                    <jats:p>2. Ravi V, Will P, Kueckelhaus J, et al. \u2018Spatially resolved multi-omics deciphers bidirectional tumor-host interdependence in glioblastoma.\u2019 Dryad 2025;10.5061\/dryad.h70rxwdmj.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bib\/bbaf631.034","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T14:13:30Z","timestamp":1765548810000},"page":"i24-i24","source":"Crossref","is-referenced-by-count":0,"title":["Spatial multi-omics characterizes neuronal mimicry as a coherent hybrid state in glioblastoma"],"prefix":"10.1093","volume":"26","author":[{"given":"Claire","family":"Yuan","sequence":"first","affiliation":[{"name":"AlphaMind Club , Birmingham, AL ,","place":["United States"]}]},{"given":"Jake Y","family":"Chen","sequence":"additional","affiliation":[{"name":"AlphaMind Club , Birmingham, AL ,","place":["United States"]},{"name":"Systems Pharmacology AI Research Center, School of Medicine, The University of Alabama at Birmingham , Birmingham, AL ,","place":["United States"]}]}],"member":"286","published-online":{"date-parts":[[2025,12,12]]},"container-title":["Briefings in Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bib\/article-pdf\/26\/Supplement_1\/i24\/65854412\/bbaf631.034.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bib\/article-pdf\/26\/Supplement_1\/i24\/65854412\/bbaf631.034.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T21:56:37Z","timestamp":1766786197000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bib\/article\/26\/Supplement_1\/i24\/8377994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":0,"journal-issue":{"issue":"Supplement_1","published-print":{"date-parts":[[2025,12,12]]}},"URL":"https:\/\/doi.org\/10.1093\/bib\/bbaf631.034","relation":{},"ISSN":["1467-5463","1477-4054"],"issn-type":[{"type":"print","value":"1467-5463"},{"type":"electronic","value":"1477-4054"}],"subject":[],"published-other":{"date-parts":[[2025,12]]},"published":{"date-parts":[[2025,12]]}}}