{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T12:57:43Z","timestamp":1765976263468},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T00:00:00Z","timestamp":1553040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Key R&D Program of China","award":["2016YFC0901701","2016YFC0901603"],"award-info":[{"award-number":["2016YFC0901701","2016YFC0901603"]}]},{"name":"\u2018863 Projects\u2019 of Ministry of Science and Technology of China","award":["2015AA020101","2015AA020108"],"award-info":[{"award-number":["2015AA020101","2015AA020108"]}]},{"name":"Key Research Program of the Chinese Academy of Sciences","award":["KJZD-EW-L14"],"award-info":[{"award-number":["KJZD-EW-L14"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peripheral blood gene expression intensity-based methods for distinguishing healthy individuals from cancer patients are limited by sensitivity to batch effects and data normalization and variability between expression profiling assays. To improve the robustness and precision of blood gene expression-based tumour detection, it is necessary to perform molecular diagnostic tests using a more stable approach. Taking breast cancer as an example, we propose a machine learning\u2013based framework that distinguishes breast cancer patients from healthy subjects by pairwise rank transformation of gene expression intensity in each sample. We showed the diagnostic potential of the method by performing RNA-seq for 37 peripheral blood samples from breast cancer patients and by collecting RNA-seq data from healthy donors in Genotype-Tissue Expression project and microarray mRNA expression datasets in Gene Expression Omnibus. The framework was insensitive to experimental batch effects and data normalization, and it can be simultaneously applied to new sample prediction.<\/jats:p>","DOI":"10.1093\/bib\/bbz027","type":"journal-article","created":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T12:07:53Z","timestamp":1552651673000},"page":"1006-1015","source":"Crossref","is-referenced-by-count":11,"title":["Platform-independent approach for cancer detection from gene expression profiles of peripheral blood cells"],"prefix":"10.1093","volume":"21","author":[{"given":"Yadong","family":"Yang","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China"}]},{"given":"Tao","family":"Zhang","sequence":"first","affiliation":[{"name":"BIG Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China"}]},{"given":"Rudan","family":"Xiao","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China"}]},{"given":"Xiaopeng","family":"Hao","sequence":"first","affiliation":[{"name":"Breast Oncology Department, Affiliated Hospital, Academy of Military Medical Sciences, Beijing, China"}]},{"given":"Huiqiang","family":"Zhang","sequence":"first","affiliation":[{"name":"Breast Oncology Department, Affiliated Hospital, Academy of Military Medical Sciences, Beijing, China"}]},{"given":"Hongzhu","family":"Qu","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China"}]},{"given":"Bingbing","family":"Xie","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China"}]},{"given":"Tao","family":"Wang","sequence":"first","affiliation":[{"name":"Breast Oncology Department, Affiliated Hospital, Academy of Military Medical Sciences, Beijing, China"}]},{"given":"Xiangdong","family":"Fang","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China"}]}],"member":"286","published-online":{"date-parts":[[2019,3,20]]},"reference":[{"key":"2020051819181137500_ref1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s13059-017-1191-5","article-title":"CancerLocator: non-invasive cancer diagnosis and tissue-of-origin prediction using methylation profiles of cell-free DNA","volume":"18","author":"Kang","year":"2017","journal-title":"Genome Biol"},{"key":"2020051819181137500_ref2","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/nmat4997","article-title":"Circulating tumour DNA methylation markers for 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