{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:33:52Z","timestamp":1772138032631,"version":"3.50.1"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2019,6,24]],"date-time":"2019-06-24T00:00:00Z","timestamp":1561334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"publisher","award":["ANR-16-CE23-0001"],"award-info":[{"award-number":["ANR-16-CE23-0001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"INCEPTION project","award":["PIA\/ANR-16-CONV-0005"],"award-info":[{"award-number":["PIA\/ANR-16-CONV-0005"]}]},{"name":"Brazilian Ministry of Science, Technology and Innovation","award":["203362\/2014-4"],"award-info":[{"award-number":["203362\/2014-4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>Nanopore long-read sequencing technology offers promising alternatives to high-throughput short read sequencing, especially in the context of RNA-sequencing. However this technology is currently hindered by high error rates in the output data that affect analyses such as the identification of isoforms, exon boundaries, open reading frames and creation of gene catalogues. Due to the novelty of such data, computational methods are still actively being developed and options for the error correction of Nanopore RNA-sequencing long reads remain limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In this article, we evaluate the extent to which existing long-read DNA error correction methods are capable of correcting cDNA Nanopore reads. We provide an automatic and extensive benchmark tool that not only reports classical error correction metrics but also the effect of correction on gene families, isoform diversity, bias toward the major isoform and splice site detection. We find that long read error correction tools that were originally developed for DNA are also suitable for the correction of Nanopore RNA-sequencing data, especially in terms of increasing base pair accuracy. Yet investigators should be warned that the correction process perturbs gene family sizes and isoform diversity. This work provides guidelines on which (or whether) error correction tools should be used, depending on the application type.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Benchmarking software<\/jats:title>\n                    <jats:p>https:\/\/gitlab.com\/leoisl\/LR_EC_analyser<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bib\/bbz058","type":"journal-article","created":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T07:21:58Z","timestamp":1556176918000},"page":"1164-1181","source":"Crossref","is-referenced-by-count":34,"title":["Comparative assessment of long-read error correction software applied to Nanopore RNA-sequencing data"],"prefix":"10.1093","volume":"21","author":[{"given":"Leandro","family":"Lima","sequence":"first","affiliation":[{"name":"Univ Lyon, Universit\u00e9 Lyon 1, CNRS, Laboratoire de Biom\u00e9trie et Biologie Evolutive UMR Villeurbanne, France"},{"name":"EPI ERABLE - Inria Grenoble, Rh\u00f4ne-Alpes, France"},{"name":"Universit\u00e0 di Roma \u2018Tor Vergata\u2019, Roma, Italy"}]},{"given":"Camille","family":"Marchet","sequence":"additional","affiliation":[{"name":"CNRS, Universit\u00e9 de Lille, CRIStAL UMR, Lille, France"}]},{"given":"S\u00e9gol\u00e8ne","family":"Caboche","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, UMR, Center for Infection and Immunity of Lille, Lille, France"}]},{"given":"Corinne","family":"Da Silva","sequence":"additional","affiliation":[{"name":"Genoscope, Institut de biologie Francois-Jacob, Commissariat \u00e0 l\u2019Energie Atomique (CEA), Universit\u00e9 Paris-Saclay, Evry, France"}]},{"given":"Benjamin","family":"Istace","sequence":"additional","affiliation":[{"name":"Genoscope, Institut de biologie Francois-Jacob, Commissariat \u00e0 l\u2019Energie Atomique (CEA), Universit\u00e9 Paris-Saclay, Evry, France"}]},{"given":"Jean-Marc","family":"Aury","sequence":"additional","affiliation":[{"name":"Genoscope, Institut de biologie Francois-Jacob, Commissariat \u00e0 l\u2019Energie Atomique (CEA), Universit\u00e9 Paris-Saclay, Evry, France"}]},{"given":"H\u00e9l\u00e8ne","family":"Touzet","sequence":"additional","affiliation":[{"name":"CNRS, Universit\u00e9 de Lille, CRIStAL UMR, Lille, France"}]},{"given":"Rayan","family":"Chikhi","sequence":"additional","affiliation":[{"name":"CNRS, Universit\u00e9 de Lille, CRIStAL UMR, Lille, France"},{"name":"Institut Pasteur, C3BI - USR 3756, 25-28 rue du Docteur Roux, Paris, France"}]}],"member":"286","published-online":{"date-parts":[[2019,6,24]]},"reference":[{"issue":"5","key":"2020080709263125900_ref1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1038\/nbt.1621","article-title":"Transcript assembly and quantification by RNA-seq reveals unannotated transcripts and isoform switching during cell differentiation","volume":"28","author":"Trapnell","year":"2010","journal-title":"Nat Biotechnol"},{"issue":"12","key":"2020080709263125900_ref2","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1038\/nbt.4020","article-title":"Accurate assembly of transcripts through phase-preserving graph decomposition","volume":"35","author":"Shao","year":"2017","journal-title":"Nat Biotechnol"},{"issue":"5","key":"2020080709263125900_ref3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/nbt.1633","article-title":"reconstruction of cell type-specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs","volume":"28","author":"Guttman","year":"2010","journal-title":"Nat Biotechnol"},{"issue":"3","key":"2020080709263125900_ref4","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat Biotechnol"},{"issue":"8","key":"2020080709263125900_ref5","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1093\/bioinformatics\/bts094","article-title":"Oases: robust de novo RNA-seq assembly across the dynamic range of expression levels","volume":"28","author":"Schulz","year":"2012","journal-title":"Bioinformatics"},{"issue":"12","key":"2020080709263125900_ref6","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1093\/bioinformatics\/btu077","article-title":"SOAPdenovo-trans: de novo transcriptome assembly with short RNA-seq reads","volume":"30","author":"Xie","year":"2014","journal-title":"Bioinformatics"},{"issue":"11","key":"2020080709263125900_ref7","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1038\/nmeth.1517","article-title":"assembly and analysis of RNA-seq data","volume":"7","author":"Robertson","year":"2010","journal-title":"Nat Methods"},{"issue":"7","key":"2020080709263125900_ref8","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat Biotechnol"},{"issue":"5","key":"2020080709263125900_ref9","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/nbt0510-421","article-title":"Advancing RNA-seq analysis","volume":"28","author":"Haas","year":"2010","journal-title":"Nat Biotechnol"},{"issue":"10","key":"2020080709263125900_ref10","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nrg3068","article-title":"Next-generation transcriptome assembly","volume":"12","author":"Martin","year":"2011","journal-title":"Nat Rev Genet"},{"issue":"13","key":"2020080709263125900_ref11","doi-asserted-by":"crossref","first-page":"2168","DOI":"10.1093\/bioinformatics\/bty098","article-title":"IDP-denovo: de novo transcriptome assembly and isoform annotation by hybrid sequencing","volume":"34","author":"Fu","year":"2018","journal-title":"Bioinformatics"},{"issue":"6","key":"2020080709263125900_ref12","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/s41576-018-0003-4","article-title":"Piercing the dark matter: bioinformatics of long-range sequencing and mapping","volume":"19","author":"Sedlazeck","year":"2018","journal-title":"Nat Rev Genet"},{"issue":"6","key":"2020080709263125900_ref13","first-page":"7","article-title":"Unveiling the complexity of the maize transcriptome by single-molecule long-read sequencing","volume":"11708","author":"Wang","year":"2016","journal-title":"Nat Commun"},{"issue":"8","key":"2020080709263125900_ref14","first-page":"3","article-title":"Long read reference genome-free reconstruction of a full-length transcriptome from Astragalus membranaceus reveals transcript variants involved in bioactive compound biosynthesis","volume":"17031","author":"Li","year":"2017","journal-title":"Cell Discov"},{"issue":"18","key":"2020080709263125900_ref15","doi-asserted-by":"crossref","first-page":"e116","DOI":"10.1093\/nar\/gkv562","article-title":"Characterization of fusion genes and the significantly expressed fusion isoforms in breast cancer by hybrid sequencing","volume":"43","author":"Weirather","year":"2015","journal-title":"Nucleic Acids Res"},{"issue":"7","key":"2020080709263125900_ref16","first-page":"8","article-title":"Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells","volume":"16027","author":"Byrne","year":"2017","journal-title":"Nat Commun"},{"issue":"1","key":"2020080709263125900_ref17","doi-asserted-by":"crossref","first-page":"31602","DOI":"10.1038\/srep31602","article-title":"Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations","volume":"6","author":"Oikonomopoulos","year":"2016","journal-title":"Sci Rep"},{"issue":"1","key":"2020080709263125900_ref18","doi-asserted-by":"crossref","first-page":"4601","DOI":"10.1038\/s41467-018-06910-x","article-title":"Deciphering highly similar multigene family transcripts from Iso-Seq data with IsoCon","volume":"9","author":"Sahlin","year":"2018","journal-title":"Nat Commun"},{"key":"2020080709263125900_ref19","first-page":"459529","article-title":"Nanopore native RNA sequencing of a human poly(A) transcriptome","author":"Workman","year":"2018"},{"issue":"1","key":"2020080709263125900_ref20","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s13742-016-0140-7","article-title":"INC-Seq: accurate single molecule reads using nanopore sequencing","volume":"5","author":"Li","year":"2016","journal-title":"GigaScience"},{"issue":"6","key":"2020080709263125900_ref21","first-page":"6","article-title":"Comprehensive comparison of Pacific biosciences and Oxford Nanopore technologies and their applications to transcriptome analysis","volume":"100","author":"Weirather","year":"2017","journal-title":"F1000Res"},{"issue":"4","key":"2020080709263125900_ref22","first-page":"7","article-title":"Fast and sensitive mapping of nanopore sequencing reads with GraphMap","volume":"11307","author":"Sovi\u0107","year":"2016","journal-title":"Nat Commun"},{"key":"2020080709263125900_ref23","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1109\/BHI.2016.7455838","article-title":"Evaluating the impact of sequencing error correction for RNA-seq data with ERCC RNA spike-in controls","volume-title":"2016 IEEE-EMBS International Conference on Biomedical and Health Informatics (BHI)","author":"Tong","year":"2016"},{"issue":"1","key":"2020080709263125900_ref24","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s13742-015-0089-y","article-title":"Rcorrector: efficient and accurate error correction for Illumina RNA-seq reads","volume":"4","author":"Song","year":"2015","journal-title":"GigaScience"},{"issue":"10","key":"2020080709263125900_ref25","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0046679","article-title":"Improving PacBio long read accuracy by short read alignment","volume":"7","author":"Au","year":"2012","journal-title":"PLoS One"},{"issue":"7","key":"2020080709263125900_ref26","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/nbt.2280","article-title":"Hybrid error correction and de novo assembly of single-molecule sequencing reads","volume":"30","author":"Koren","year":"2012","journal-title":"Nat Biotechnol"},{"issue":"1","key":"2020080709263125900_ref27","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1186\/s12859-017-1610-3","article-title":"HALC: high throughput algorithm for long read error correction","volume":"18","author":"Bao","year":"2017","journal-title":"BMC Bioinformatics"},{"issue":"24","key":"2020080709263125900_ref28","doi-asserted-by":"crossref","first-page":"3506","DOI":"10.1093\/bioinformatics\/btu538","article-title":"LoRDEC: accurate and efficient long read error correction","volume":"30","author":"Salmela","year":"2014","journal-title":"Bioinformatics"},{"issue":"1","key":"2020080709263125900_ref29","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1186\/s12864-015-1519-z","article-title":"Genome assembly using Nanopore-guided long and error-free DNA reads","volume":"16","author":"Madoui","year":"2015","journal-title":"BMC Genomics"},{"issue":"21","key":"2020080709263125900_ref30","doi-asserted-by":"crossref","first-page":"3004","DOI":"10.1093\/bioinformatics\/btu392","article-title":"Proovread: large-scale high-accuracy PacBio correction through iterative short read consensus","volume":"30","author":"Hackl","year":"2014","journal-title":"Bioinformatics"},{"issue":"5","key":"2020080709263125900_ref31","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1101\/gr.215087.116","article-title":"Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation","volume":"27","author":"Koren","year":"2017","journal-title":"Genome Res"},{"key":"2020080709263125900_ref32","first-page":"106252","article-title":"Non hybrid long read consensus using local de Bruijn graph assembly","author":"Tischler","year":"2017"},{"issue":"6","key":"2020080709263125900_ref33","doi-asserted-by":"crossref","first-page":"btw321","DOI":"10.1093\/bioinformatics\/btw321","article-title":"Accurate self-correction of errors in long reads using de Bruijn graphs","volume":"33","author":"Salmela","year":"2016","journal-title":"Bioinformatics"},{"issue":"11","key":"2020080709263125900_ref34","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1038\/nmeth.4432","article-title":"MECAT: fast mapping, error correction, and de novo assembly for single-molecule sequencing reads","volume":"14","author":"Xiao","year":"2017","journal-title":"Nat Methods"},{"issue":"6","key":"2020080709263125900_ref35","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/nmeth.2474","article-title":"Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data","volume":"10","author":"Chin","year":"2013","journal-title":"Nat Methods"},{"issue":"8","key":"2020080709263125900_ref36","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1038\/nmeth.3444","article-title":"A complete bacterial genome assembled de novo using only nanopore sequencing data","volume":"12","author":"Loman","year":"2015","journal-title":"Nat Methods"},{"issue":"12","key":"2020080709263125900_ref37","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1038\/nmeth.4035","article-title":"Phased diploid genome assembly with single-molecule real-time sequencing","volume":"13","author":"Chin","year":"2016","journal-title":"Nat Methods"},{"issue":"1","key":"2020080709263125900_ref38","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1186\/s12859-016-1316-y","article-title":"LSCplus: a fast solution for improving long read accuracy by short read alignment","volume":"17","author":"Hu","year":"2016","journal-title":"BMC Bioinformatics"},{"key":"2020080709263125900_ref39","article-title":"Hybrid correction of highly noisy long reads using a variable-order de Bruijn graph","volume":"6","author":"Morisse","year":"2018","journal-title":"Bioinformatics"},{"issue":"1","key":"2020080709263125900_ref40","doi-asserted-by":"crossref","first-page":"9936","DOI":"10.1038\/s41598-018-28364-3","article-title":"HECIL: a hybrid error correction algorithm for long reads with iterative learning","volume":"8","author":"Choudhury","year":"2018","journal-title":"Sci Rep"},{"key":"2020080709263125900_ref41","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1109\/DEXA.2016.032","article-title":"Efficient hybrid De novo error correction and assembly for long reads","volume-title":"2016 27th International Workshop on Database and Expert Systems Applications (DEXA)","author":"Kchouk","year":"2016"},{"issue":"1","key":"2020080709263125900_ref42","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13015-016-0075-7","article-title":"Jabba: hybrid error correction for long sequencing reads","volume":"11","author":"Miclotte","year":"2016","journal-title":"Algorithms Mol Biol"},{"issue":"11","key":"2020080709263125900_ref43","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1101\/gr.191395.115","article-title":"Oxford Nanopore sequencing, hybrid error correction, and de novo assembly of a eukaryotic genome","volume":"25","author":"Goodwin","year":"2015","journal-title":"Genome Res"},{"issue":"5","key":"2020080709263125900_ref44","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1101\/gr.214270.116","article-title":"Fast and accurate de novo genome assembly from long uncorrected reads","volume":"27","author":"Vaser","year":"2017","journal-title":"Genome Res"},{"issue":"22","key":"2020080709263125900_ref45","doi-asserted-by":"crossref","first-page":"3652","DOI":"10.1093\/bioinformatics\/btx489","article-title":"LRCstats, a tool for evaluating long reads correction methods","volume":"33","author":"La","year":"2017","journal-title":"Bioinformatics"},{"key":"2020080709263125900_ref46","first-page":"512889","article-title":"Elector: evaluator for long reads correction methods","author":"Marchet","year":"2019"},{"key":"2020080709263125900_ref47","volume-title":"Evaluation of long read error correction software. Technical Report, INRIA Rennes - Bretagne Atlantique; GenScale","author":"Bouri","year":"2017"},{"issue":"1","key":"2020080709263125900_ref48","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s13059-018-1605-z","article-title":"A comparative evaluation of hybrid error correction methods for error-prone long reads","volume":"20","author":"Fu","year":"2019","journal-title":"Genome Biol"},{"issue":"5","key":"2020080709263125900_ref49","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1093\/bioinformatics\/btx668","article-title":"Evaluation of tools for long read RNA-seq splice-aware alignment","volume":"34","author":"Kri\u017eanovi\u0107","year":"2018","journal-title":"Bioinformatics"},{"issue":"6","key":"2020080709263125900_ref50","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3390\/ijms18061110","article-title":"Validation of splicing events in Transcriptome sequencing data","volume":"18","author":"Kaisers","year":"2017","journal-title":"Int J Mol Sci"},{"issue":"9","key":"2020080709263125900_ref51","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1093\/bioinformatics\/bti310","article-title":"GMAP: a genomic mapping and alignment program for mRNA and EST sequences","volume":"21","author":"Wu","year":"2005","journal-title":"Bioinformatics"},{"issue":"18","key":"2020080709263125900_ref52","doi-asserted-by":"crossref","first-page":"3094","DOI":"10.1093\/bioinformatics\/bty191","article-title":"Minimap2: pairwise alignment for nucleotide sequences","volume":"34","author":"Li","year":"2018","journal-title":"Bioinformatics"},{"issue":"23","key":"2020080709263125900_ref53","doi-asserted-by":"crossref","first-page":"3399","DOI":"10.1093\/bioinformatics\/btu555","article-title":"Poretools: a toolkit for analyzing nanopore sequence data","volume":"30","author":"Loman","year":"2014","journal-title":"Bioinformatics"},{"issue":"4","key":"2020080709263125900_ref54","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1101\/gr.229202","article-title":"BLAT\u2014the BLAST-like alignment tool","volume":"12","author":"Kent","year":"2002","journal-title":"Genome Res"},{"issue":"4","key":"2020080709263125900_ref55","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1093\/bioinformatics\/13.4.477","article-title":"EST_GENOME: a program to align spliced DNA sequences to unspliced genomic DNA","volume":"13","author":"Mott","year":"1997","journal-title":"Bioinformatics"},{"issue":"1","key":"2020080709263125900_ref56","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/nbt.1754","article-title":"Integrative genomics viewer","volume":"29","author":"Robinson","year":"2011","journal-title":"Nat Biotechnol"},{"issue":"2","key":"2020080709263125900_ref57","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1093\/bib\/bbs017","article-title":"Integrative genomics viewer (IGV): high-performance genomics data visualization and exploration","volume":"14","author":"Thorvaldsdottir","year":"2012","journal-title":"Brief Bioinform"},{"issue":"4","key":"2020080709263125900_ref58","first-page":"387","article-title":"Distributions of exons and introns in the human genome","volume":"4","author":"Sakharkar","year":"2004","journal-title":"In Silico Biol"},{"issue":"12","key":"2020080709263125900_ref59","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0082138","article-title":"SSW library: an SIMD smith-waterman C\/C++ library for use in genomic applications","volume":"8","author":"Zhao","year":"2013","journal-title":"PLoS One"}],"container-title":["Briefings in Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/bib\/article-pdf\/21\/4\/1164\/33582772\/bbz058.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/bib\/article-pdf\/21\/4\/1164\/33582772\/bbz058.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T09:32:40Z","timestamp":1596792760000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bib\/article\/21\/4\/1164\/5512144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,24]]},"references-count":59,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,6,24]]},"published-print":{"date-parts":[[2020,7,15]]}},"URL":"https:\/\/doi.org\/10.1093\/bib\/bbz058","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/476622","asserted-by":"object"}]},"ISSN":["1477-4054"],"issn-type":[{"value":"1477-4054","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,7]]},"published":{"date-parts":[[2019,6,24]]}}}