{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T00:33:33Z","timestamp":1772843613749,"version":"3.50.1"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T00:00:00Z","timestamp":1578873600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council Fellowship","doi-asserted-by":"crossref","award":["EP\/S003002\/1"],"award-info":[{"award-number":["EP\/S003002\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Biotechnology and Biological Sciences Research Council Research"},{"name":"Royal Society Wolfson Research Merit Award"},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/N013573\/1"],"award-info":[{"award-number":["EP\/N013573\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>In experimental protein engineering, alanine-scanning mutagenesis involves the replacement of selected residues with alanine to determine the energetic contribution of each side chain to forming an interaction. For example, it is often used to study protein\u2013protein interactions. However, such experiments can be time-consuming and costly, which has led to the development of programmes for performing computational alanine-scanning mutagenesis (CASM) to guide experiments. While programmes are available for this, there is a need for a real-time web application that is accessible to non-expert users.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Here, we present BAlaS, an interactive web application for performing CASM via BudeAlaScan and visualizing its results. BAlaS is interactive and intuitive to use. Results are displayed directly in the browser for the structure being interrogated enabling their rapid inspection. BAlaS has broad applications in areas, such as drug discovery and protein-interface design.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>BAlaS works on all modern browsers and is available through the following website: https:\/\/balas.app. The project is open source, distributed using an MIT license and is available on GitHub (https:\/\/github.com\/wells-wood-research\/balas).<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btaa026","type":"journal-article","created":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T20:10:08Z","timestamp":1578600608000},"page":"2917-2919","source":"Crossref","is-referenced-by-count":76,"title":["BAlaS: fast, interactive and accessible computational alanine-scanning using BudeAlaScan"],"prefix":"10.1093","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1243-3105","authenticated-orcid":false,"given":"Christopher W","family":"Wood","sequence":"first","affiliation":[{"name":"School of Biological Sciences , University of Edinburgh, Edinburgh EH9 3FF, UK"}]},{"given":"Amaurys A","family":"Ibarra","sequence":"additional","affiliation":[{"name":"School of Biochemistry , University of Bristol, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK"}]},{"given":"Gail J","family":"Bartlett","sequence":"additional","affiliation":[{"name":"School of Chemistry , University of Bristol, Bristol BS8 1TS, UK"}]},{"given":"Andrew J","family":"Wilson","sequence":"additional","affiliation":[{"name":"School of Chemistry"},{"name":"Astbury Centre for Structural Molecular Biology , University of Leeds, Leeds LS2 9JT, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0394-3202","authenticated-orcid":false,"given":"Derek N","family":"Woolfson","sequence":"additional","affiliation":[{"name":"School of Biochemistry , University of Bristol, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK"},{"name":"School of Chemistry , University of Bristol, Bristol BS8 1TS, UK"},{"name":"BrisSynBio , University of Bristol, Life Sciences Building, Bristol BS8 1TQ, UK"}]},{"given":"Richard B","family":"Sessions","sequence":"additional","affiliation":[{"name":"School of Biochemistry , University of Bristol, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK"},{"name":"BrisSynBio , University of Bristol, Life Sciences Building, Bristol BS8 1TQ, UK"}]}],"member":"286","published-online":{"date-parts":[[2020,1,13]]},"reference":[{"key":"2023013111491339900_btaa026-B1","author":"Biasini","year":"2015"},{"key":"2023013111491339900_btaa026-B2","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1126\/science.7529940","article-title":"A hot spot of binding energy in a hormone-receptor interface","volume":"267","author":"Clackson","year":"1995","journal-title":"Science"},{"key":"2023013111491339900_btaa026-B3","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1126\/science.2471267","article-title":"High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis","volume":"244","author":"Cunningham","year":"1989","journal-title":"Science"},{"key":"2023013111491339900_btaa026-B4","doi-asserted-by":"crossref","first-page":"W333","DOI":"10.1093\/nar\/gkt450","article-title":"BeAtMuSiC: prediction of changes in protein\u2013protein binding affinity on mutations","volume":"41","author":"Dehouck","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"2023013111491339900_btaa026-B5","first-page":"2252","article-title":"Predicting and experimentally validating hot-spot residues at protein\u2013protein interfaces","volume":"14","author":"Ibarra","year":"2019","journal-title":"ACS Chem. 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