{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T04:22:28Z","timestamp":1775190148363,"version":"3.50.1"},"reference-count":3,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T00:00:00Z","timestamp":1580083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["18-15548S"],"award-info":[{"award-number":["18-15548S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"name":"SYMBIT project","award":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000477"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/15_003\/0000477"]}]},{"DOI":"10.13039\/501100008530","name":"ERDF","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"Abstract<\/jats:title>\n \n Motivation<\/jats:title>\n G-quadruplexes (G4) are important regulatory non-B DNA structures with therapeutic potential. A tool for rational design of mutations leading to decreased propensity for G4 formation should be useful in studying G4 functions. Although tools exist for G4 prediction, no easily accessible tool for the rational design of G4 mutations has been available.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n We developed a web-based tool termed G4Killer that is based on the G4Hunter algorithm. This new tool is a platform-independent and user-friendly application to design mutations crippling G4 propensity in a parsimonious way (i.e., keeping the primary sequence as close as possible to the original one). The tool is integrated into our DNA analyzer server and allows for generating mutated DNA sequences having the desired lowered G4Hunter score with minimal mutation steps.<\/jats:p>\n <\/jats:sec>\n \n Availability and implementation<\/jats:title>\n The G4Killer web tool can be accessed at: http:\/\/bioinformatics.ibp.cz.<\/jats:p>\n <\/jats:sec>\n \n Supplementary information<\/jats:title>\n Supplementary data are available at Bioinformatics online.<\/jats:p>\n <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btaa057","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T20:10:09Z","timestamp":1579637409000},"page":"3246-3247","source":"Crossref","is-referenced-by-count":13,"title":["G4Killer web application: a tool to design G-quadruplex mutations"],"prefix":"10.1093","volume":"36","author":[{"given":"Vaclav","family":"Brazda","sequence":"first","affiliation":[{"name":"Institute of Biophysics of the Czech Academy of Sciences , Brno 612 65, Czech Republic"}]},{"given":"Jan","family":"Kolomaznik","sequence":"additional","affiliation":[{"name":"Department of Informatics , Mendel University in Brno, Brno 613 00, Czech Republic"}]},{"given":"Jean-Louis","family":"Mergny","sequence":"additional","affiliation":[{"name":"Institute of Biophysics of the Czech Academy of Sciences , Brno 612 65, Czech Republic"}]},{"given":"Jiri","family":"Stastny","sequence":"additional","affiliation":[{"name":"Department of Informatics , Mendel University in Brno, Brno 613 00, Czech Republic"}]}],"member":"286","published-online":{"date-parts":[[2020,1,27]]},"reference":[{"key":"2023013112025480300_btaa057-B1","doi-asserted-by":"crossref","first-page":"3493","DOI":"10.1093\/bioinformatics\/btz087","article-title":"G4Hunter web application: a web server for G-quadruplex prediction","volume":"35","author":"Br\u00e1zda","year":"2019","journal-title":"Bioinformatics"},{"key":"2023013112025480300_btaa057-B2","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1093\/nar\/gkl1057","article-title":"G-quadruplexes in promoters throughout the human genome","volume":"35","author":"Huppert","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"2023013112025480300_btaa057-B3","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1038\/ncomms2792","article-title":"G-quadruplex structures are stable and detectable in human genomic DNA","volume":"4","author":"Lam","year":"2013","journal-title":"Nat. Commun"}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/bioinformatics\/advance-article-pdf\/doi\/10.1093\/bioinformatics\/btaa057\/32471671\/btaa057.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/36\/10\/3246\/48990868\/bioinformatics_36_10_3246.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/36\/10\/3246\/48990868\/bioinformatics_36_10_3246.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T18:36:40Z","timestamp":1675190200000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/36\/10\/3246\/5716328"}},"subtitle":[],"editor":[{"given":"Alfonso","family":"Valencia","sequence":"additional","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2020,1,27]]},"references-count":3,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,5,1]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btaa057","relation":{},"ISSN":["1367-4803","1367-4811"],"issn-type":[{"value":"1367-4803","type":"print"},{"value":"1367-4811","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,5,15]]},"published":{"date-parts":[[2020,1,27]]}}}