{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T08:14:58Z","timestamp":1768464898641,"version":"3.49.0"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T00:00:00Z","timestamp":1582848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFA0904300"],"award-info":[{"award-number":["2019YFA0904300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0900700"],"award-info":[{"award-number":["2018YFA0900700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFC1601702"],"award-info":[{"award-number":["2017YFC1601702"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31700081"],"award-info":[{"award-number":["31700081"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31570092"],"award-info":[{"award-number":["31570092"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific Research Conditions and Technical Support System Program","award":["ZSYS-016"],"award-info":[{"award-number":["ZSYS-016"]}]},{"name":"Science and Technology Service Network Initiative"},{"name":"Chinese Academy of Sciences of China","award":["QYZDB-SSW-SMC012"],"award-info":[{"award-number":["QYZDB-SSW-SMC012"]}]},{"name":"International Partnership Program of Chinese Academy of Sciences of China","award":["153D31KYSB20170121"],"award-info":[{"award-number":["153D31KYSB20170121"]}]},{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin","doi-asserted-by":"publisher","award":["15JCYBJC54300"],"award-info":[{"award-number":["15JCYBJC54300"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>The development of sequencing technologies has generated large amounts of protein sequence data. The automated prediction of the enzymatic reactions of uncharacterized proteins is a major challenge in the field of bioinformatics. Here, we present Bio2Rxn as a web-based tool to provide putative enzymatic reaction predictions for uncharacterized protein sequences. Bio2Rxn adopts a consensus strategy by incorporating six types of enzyme prediction tools. It allows for the efficient integration of these computational resources to maximize the accuracy and comprehensiveness of enzymatic reaction predictions, which facilitates the characterization of the functional roles of target proteins in metabolism. Bio2Rxn further links the enzyme function prediction with more than 300 000 enzymatic reactions, which were manually curated by more than 100 people over the past 9\u2009years from more than 580 000 publications.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>Bio2Rxn is available at: http:\/\/design.rxnfinder.org\/bio2rxn\/.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Contact<\/jats:title>\n                  <jats:p>qnhu@sibs.ac.cn<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btaa135","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T20:16:28Z","timestamp":1582661788000},"page":"3600-3601","source":"Crossref","is-referenced-by-count":16,"title":["Bio2Rxn: sequence-based enzymatic reaction predictions by a consensus strategy"],"prefix":"10.1093","volume":"36","author":[{"given":"Tong","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Biotechnology , Tianjin University of Science and Technology, Tianjin 300457, China"},{"name":"Tianjin Institute of Industrial Biotechnology , Chinese Academy of Sciences, Tianjin 300308, China"}]},{"given":"Yu","family":"Tian","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Industrial Biotechnology , Chinese Academy of Sciences, Tianjin 300308, China"},{"name":"University of Chinese Academy of Sciences , Beijing 100864, China"}]},{"given":"Le","family":"Yuan","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Industrial Biotechnology , Chinese Academy of Sciences, Tianjin 300308, China"}]},{"given":"Fu","family":"Chen","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Industrial Biotechnology , Chinese Academy of Sciences, Tianjin 300308, China"}]},{"given":"Ailin","family":"Ren","sequence":"additional","affiliation":[{"name":"Tianjin Institute of Industrial Biotechnology , Chinese Academy of Sciences, Tianjin 300308, China"},{"name":"University of Chinese Academy of Sciences , Beijing 100864, China"}]},{"given":"Qian-Nan","family":"Hu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Computational Biology , CAS-MPG Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200333, China"}]}],"member":"286","published-online":{"date-parts":[[2020,2,28]]},"reference":[{"key":"2023062312021995300_btaa135-B1","doi-asserted-by":"crossref","first-page":"e0153503","DOI":"10.1371\/journal.pone.0153503","article-title":"Identification of multi-functional enzyme with multi-label classifier","volume":"11","author":"Che","year":"2016","journal-title":"PLoS One"},{"key":"2023062312021995300_btaa135-B2","article-title":"Data-driven rational biosynthesis design: from molecules to cell factories","author":"Chen","year":"2019","journal-title":"Brief Bioinform,"},{"key":"2023062312021995300_btaa135-B3","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1186\/s12859-018-2368-y","article-title":"ECPred: a tool for the prediction of the enzymatic functions of protein sequences based on the EC nomenclature","volume":"19","author":"Dalkiran","year":"2018","journal-title":"BMC Bioinformatics"},{"key":"2023062312021995300_btaa135-B4","doi-asserted-by":"crossref","first-page":"e0182216","DOI":"10.1371\/journal.pone.0182216","article-title":"BrEPS 2.0: optimization of sequence pattern prediction for enzyme annotation","volume":"12","author":"Dudek","year":"2017","journal-title":"PLoS One"},{"key":"2023062312021995300_btaa135-B5","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0167-4838(91)90544-A","article-title":"Purification and characterization of a catalase-peroxidase and a typical catalase from the bacterium Klebsiella pneumoniae","volume":"1077","author":"Hochman","year":"1991","journal-title":"Biochim Biophys Acta"},{"key":"2023062312021995300_btaa135-B6","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1093\/bioinformatics\/btr413","article-title":"RxnFinder: biochemical reaction search engines using molecular structures, molecular fragments and reaction similarity","volume":"27","author":"Hu","year":"2011","journal-title":"Bioinformatics"},{"key":"2023062312021995300_btaa135-B7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1687-4153-2012-1","article-title":"A top-down approach to classify enzyme functional classes and sub-classes using random forest","volume":"2012","author":"Kumar","year":"2012","journal-title":"EURASIP J. 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