{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T00:38:47Z","timestamp":1740184727297,"version":"3.37.3"},"reference-count":2,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T00:00:00Z","timestamp":1606262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100019701","name":"CeBiB","doi-asserted-by":"crossref","award":["PIA FB0001"],"award-info":[{"award-number":["PIA FB0001"]}],"id":[{"id":"10.13039\/501100019701","id-type":"DOI","asserted-by":"crossref"}]},{"name":"PAI Programme","award":["I7818010006"],"award-info":[{"award-number":["I7818010006"]}]},{"name":"PhD Scholarship","award":["21182123"],"award-info":[{"award-number":["21182123"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>BRENDA is the largest enzyme functional database, containing information of 84\u00a0000 experimentally characterized enzyme entries. This database is an invaluable resource for researchers in the biological field, which classifies enzyme-related information in categories that are very useful to obtain specific functional and protein engineering information for enzyme families. However, the BRENDA web interface, the most used by researchers with a non-informatic background, does not allow the user to cross-reference data from different categories or sub-categories in the database. Obtaining information in an easy and fast way, in a friendly web interface, without the necessity to have a deep informatics knowledge, will facilitate and improve research in the enzymology and protein engineering field.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We developed the Brenda Easy Search Tool (BEST), an interactive Shiny\/R application that enables querying the BRENDA database for complex cross-tabulated characteristics, and retrieving enzyme-related parameters and information readily and efficiently, which can be used for the study of enzyme function or as an input for other bioinformatics tools.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>BEST and its tutorial are freely available from https:\/\/pesb2.cl\/best\/.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btaa848","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T11:37:07Z","timestamp":1600429027000},"page":"1480-1481","source":"Crossref","is-referenced-by-count":0,"title":["BEST: a Shiny\/R web-based application to easily retrieve cross-related enzyme functional parameters and information from BRENDA"],"prefix":"10.1093","volume":"37","author":[{"given":"Juan Saez","family":"Hidalgo","sequence":"first","affiliation":[{"name":"University of Chile Centre for Biotechnology and Bioengineering \u2013 CeBiB, Department of Chemical Engineering, Biotechnology and Materials, , 8370456 Santiago, Chile"},{"name":"University of Chile Department of Computer Science, , 8370459 Santiago, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1403-3917","authenticated-orcid":false,"given":"Karen Y","family":"Or\u00f3stica","sequence":"additional","affiliation":[{"name":"University of Chile Centre for Biotechnology and Bioengineering \u2013 CeBiB, Department of Chemical Engineering, Biotechnology and Materials, , 8370456 Santiago, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6769-3281","authenticated-orcid":false,"given":"Anamaria","family":"Sanchez\u2013Daza","sequence":"additional","affiliation":[{"name":"University of Chile Centre for Biotechnology and Bioengineering \u2013 CeBiB, Department of Chemical Engineering, Biotechnology and Materials, , 8370456 Santiago, Chile"},{"name":"Faculty of Physical and Mathematical Sciences, University of Chile, Institute for Cell Dynamics and Biotechnology (ICDB) , 8370450 Santiago, Chile"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6053-7019","authenticated-orcid":false,"given":"\u00c1lvaro","family":"Olivera\u2013Nappa","sequence":"additional","affiliation":[{"name":"University of Chile Centre for Biotechnology and Bioengineering \u2013 CeBiB, Department of Chemical Engineering, Biotechnology and Materials, , 8370456 Santiago, Chile"}]}],"member":"286","published-online":{"date-parts":[[2020,11,25]]},"reference":[{"key":"2023051709350625400_btaa848-B1","first-page":"542","article-title":"BRENDA in 2019: a European ELIXIR core data resource","volume-title":"Nucleic Acids Res.","author":"Jeske","year":"2019"},{"key":"2023051709350625400_btaa848-B2","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.jbiotec.2017.04.020","article-title":"The BRENDA enzyme information system-From a database to an expert system","volume":"261","author":"Schomburg","year":"2017","journal-title":"J. Biotechnol"}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/bioinformatics\/advance-article-pdf\/doi\/10.1093\/bioinformatics\/btaa848\/34542393\/btaa848.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/37\/10\/1480\/50361117\/btaa848.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/37\/10\/1480\/50361117\/btaa848.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T09:50:26Z","timestamp":1684317026000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/37\/10\/1480\/5913389"}},"subtitle":[],"editor":[{"given":"Wren","family":"Jonathan","sequence":"additional","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2020,11,25]]},"references-count":2,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,6,16]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btaa848","relation":{},"ISSN":["1367-4803","1367-4811"],"issn-type":[{"type":"print","value":"1367-4803"},{"type":"electronic","value":"1367-4811"}],"subject":[],"published-other":{"date-parts":[[2021,5,15]]},"published":{"date-parts":[[2020,11,25]]}}}